%0 Journal Article %A Xuan, Shi-Hai %A Zhou, Yu-Gui %A Shao, Bo %A Cui, Ya-Lin %A Li, Jian %A Yin, Hong-Bo %A Song, Xiao-Ping %A Cong, Hui %A Jing, Feng-Xiang %A Jin, Qing-Hui %A Wang, Hui-Min %A Zhou, Jie %T Enzymic colorimetry-based DNA chip: a rapid and accurate assay for detecting mutations for clarithromycin resistance in the 23S rRNA gene of Helicobacter pylori %D 2009 %J Journal of Medical Microbiology, %V 58 %N 11 %P 1443-1448 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.010785-0 %K PC, positive control %K NC, negative control %K CAM, clarithromycin %K SNP, single-nucleotide polymorphism %I Microbiology Society, %X Macrolide drugs, such as clarithromycin (CAM), are a key component of many combination therapies used to eradicate Helicobacter pylori. However, resistance to CAM is increasing in H. pylori and is becoming a serious problem in H. pylori eradication therapy. CAM resistance in H. pylori is mostly due to point mutations (A2142G/C, A2143G) in the peptidyltransferase-encoding region of the 23S rRNA gene. In this study an enzymic colorimetry-based DNA chip was developed to analyse single-nucleotide polymorphisms of the 23S rRNA gene to determine the prevalence of mutations in CAM-related resistance in H. pylori-positive patients. The results of the colorimetric DNA chip were confirmed by direct DNA sequencing. In 63 samples, the incidence of the A2143G mutation was 17.46 % (11/63). The results of the colorimetric DNA chip were concordant with DNA sequencing in 96.83 % of results (61/63). The colorimetric DNA chip could detect wild-type and mutant signals at every site, even at a DNA concentration of 1.53×102 copies μl−1. Thus, the colorimetric DNA chip is a reliable assay for rapid and accurate detection of mutations in the 23S rRNA gene of H. pylori that lead to CAM-related resistance, directly from gastric tissues. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.010785-0