1887

Abstract

Real-time PCR was employed to detect a region of the gene, a gene of unknown function, in clinical samples. Amplification of DNA and signal production from 15 other species of human mycoplasmas and 14 other bacteria and viruses did not occur. Using a panel of 208 genital and rectal samples, the sensitivity when compared to the modified gene (encoding the major surface protein MgPa) real-time PCR assay was found to be 100 % and the specificity of the assay 99.5 % with a positive predictive value of 80 % and a negative predictive value of 100 %. The gene was found to be in all strains of and was highly conserved. was detected in 3.9 % (11/280, 95 % CI 2.1–6.9) of all male specimens, in 7.7 % (10/130, 95 % CI 4.1–13.7) of patients with non-gonococcal urethritis (NGU) and in 0.7 % (1/150, 95 % CI <0.01–4.1) of patients without urethritis. The presence of was significantly associated with NGU ( ≤0.01; 95 % Cl 0.88–0.98) and non-chlamydial-non-gonococcal urethritis (=0.0005; 95 % Cl 0.84–0.97).

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.009977-0
2009-07-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jmm/58/7/895.html?itemId=/content/journal/jmm/10.1099/jmm.0.009977-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  2. Baseman, J. B., Dallo, S. F., Tully, J. G. & Rose, D. L. ( 1988; ). Isolation and characterization of Mycoplasma genitalium strains from the human respiratory tract. J Clin Microbiol 26, 2266–2269.
    [Google Scholar]
  3. Björnelius, E., Lidbrink, P. & Jensen, J. S. ( 2000; ). Mycoplasma genitalium in non-gonococcal urethritis: a study in Swedish male STD patients. Int J STD AIDS 11, 292–296.[CrossRef]
    [Google Scholar]
  4. Blom, N., Gammeltoft, S. & Brunak, S. ( 1999; ). Sequence and structure-based prediction of eukaryotic protein phosphorylation sites. J Mol Biol 294, 1351–1362.[CrossRef]
    [Google Scholar]
  5. Burgos, R., Pich, O. Q., Querol, E. & Piñol, J. ( 2007; ). Functional analysis of the Mycoplasma genitalium MG312 protein reveals a specific requirement of the MG312 N-terminal domain for gliding motility. J Bacteriol 189, 7014–7023.[CrossRef]
    [Google Scholar]
  6. Chenna, R., Sugawara, H., Koike, T., Lopez, R., Gibson, T. J., Higgins, D. G. & Thompson, J. D. ( 2003; ). Multiple sequence alignment with the clustal series of programs. Nucleic Acids Res 31, 3497–3500.[CrossRef]
    [Google Scholar]
  7. Clausen, H. F., Fedder, J., Drasbek, M., Nielsen, P. K., Toft, B., Ingerslev, H. J., Birkelund, S. & Christiansen, G. ( 2001; ). Serological investigation of Mycoplasma genitalium in infertile women. Hum Reprod 16, 1866–1874.[CrossRef]
    [Google Scholar]
  8. Deguchi, T., Yoshida, T., Yokoi, S., Ito, M., Tamaki, M., Ishiko, H. & Maeda, S.-I. ( 2002; ). Longitudinal quantitative detection by real-time PCR of Mycoplasma genitalium in first-pass urine of men with recurrent nongonococcal urethritis. J Clin Microbiol 40, 3854–3856.[CrossRef]
    [Google Scholar]
  9. Dhandayuthapani, S., Rasmussen, W. G. & Baseman, J. B. ( 2002; ). Stability of cytadherence-related proteins P140/P110 in Mycoplasma genitalium requires MG218 and unidentified factors. Arch Med Res 33, 1–5.[CrossRef]
    [Google Scholar]
  10. Edberg, A., Jurstrand, M., Johansson, E., Wikander, E., Höög, A., Ahlqvist, T., Falk, L., Jensen, J. S. & Fredlund, H. ( 2008; ). A comparative study of three different PCR assays for detection of Mycoplasma genitalium in urogenital specimens from men and women. J Med Microbiol 57, 304–309.[CrossRef]
    [Google Scholar]
  11. Gnad, F., Ren, S., Cox, J., Olsen, J. V., Macek, B., Oroshi, M. & Mann, M. ( 2007; ). PHOSIDA (phosphorylation site database): management, structural and evolutionary investigation, and prediction of phosphosites. Genome Biol 8, R250 [CrossRef]
    [Google Scholar]
  12. Hjorth, S. V., Björnelius, E., Lidbrink, P., Falk, L., Dohn, B., Berthelsen, L., Ma, L., Martin, D. H. & Jensen, J. S. ( 2006; ). Sequence-based typing of Mycoplasma genitalium reveals sexual transmission. J Clin Microbiol 44, 2078–2083.[CrossRef]
    [Google Scholar]
  13. Horner, P. J., Gilroy, C. B., Thomas, B. J., Naidoo, R. O. M. & Taylor-Robinson, D. ( 1993; ). Association of Mycoplasma genitalium with acute non-gonococcal urethritis. Lancet 342, 582–585.[CrossRef]
    [Google Scholar]
  14. Jensen, J. S. ( 2006; ). Mycoplasma genitalium infections. Diagnosis, clinical aspects, and pathogenesis. Dan Med Bull 53, 1–27.
    [Google Scholar]
  15. Jensen, J. S., Björnelius, E., Dohn, B. & Lidbrink, P. ( 2004; ). Use of TaqMan 5′ nuclease real-time PCR for quantitative detection of Mycoplasma genitalium DNA in males with and without urethritis who were attendees at a sexually transmitted disease clinic. J Clin Microbiol 42, 683–692.[CrossRef]
    [Google Scholar]
  16. Keane, F. E., Thomas, B. J., Gilroy, C. B., Renton, A. & Taylor-Robinson, D. ( 2000; ). The association of Chlamydia trachomatis and Mycoplasma genitalium with non-gonococcal urethritis: observations on heterosexual men and their female partners. Int J STD AIDS 11, 435–459.[CrossRef]
    [Google Scholar]
  17. Lind, K., Lindhardt, B. O., Schütten, H. J., Blom, J. & Christiansen, C. ( 1984; ). Serological cross-reactions between Mycoplasma genitalium and Mycoplasma pneumoniae. J Clin Microbiol 20, 1036–1043.
    [Google Scholar]
  18. Longo, M. C., Berninger, M. S. & Hartley, J. L. ( 1990; ). Use of uracil DNA glycosylate to control carry-over in polymerase chain reaction. Gene 93, 125–128.[CrossRef]
    [Google Scholar]
  19. Ma, L., Taylor, S., Jensen, J. S., Myers, L., Lillis, R. & Martin, D. H. ( 2008; ). Short tandem repeat sequences in the Mycoplasma genitalium genome and their use in a multilocus genotyping system. BMC Microbiol 8, 130 [CrossRef]
    [Google Scholar]
  20. Maeda, S. I., Tamaki, M., Kojima, K., Yoshida, T., Ishiko, H., Yasuda, M. & Deguchi, T. ( 2001; ). Association of Mycoplasma genitalium persistence in the urethra with recurrence of nongonococcal urethritis. Sex Transm Dis 28, 472–476.[CrossRef]
    [Google Scholar]
  21. Moi, H., Reinton, N. & Moghaddam, A. ( 2009; ). Mycoplasma genitalium is associated with symptomatic and asymptomatic non-gonococcal urethritis in men. Sex Transm Infect 85, 15–18.[CrossRef]
    [Google Scholar]
  22. Musatovova, O., Dhandayuthapani, S. & Baseman, J. B. ( 2003; ). Transcriptional starts for cytadherence-related operons of Mycoplasma genitalium. FEMS Microbiol Lett 229, 73–81.[CrossRef]
    [Google Scholar]
  23. Pich, O. Q., Burgos, R., Ferrer-Navarro, M., Querol, E. & Piñol, J. ( 2008; ). Role of Mycoplasma genitalium MG218 and MG317 cytoskeletal proteins in terminal organelle organization, gliding motility and cytadherence. Microbiology 154, 3188–3198.[CrossRef]
    [Google Scholar]
  24. Siew, N., Azaria, Y. & Fischer, D. ( 2004; ). The ORFanage: an ORFan database. Nucleic Acids Res 32, D281–D283.[CrossRef]
    [Google Scholar]
  25. Su, H. C., Hutchison, C. A., III & Giddings, M. C. ( 2007; ). Mapping phosphoproteins in Mycoplasma genitalium and Mycoplasma pneumoniae. BMC Microbiol 7, 63 [CrossRef]
    [Google Scholar]
  26. Taylor-Robinson, D., Furr, P. M. & Hanna, N. F. ( 1985; ). Microbiological and serological study of non-gonococcal urethritis with special reference to Mycoplasma genitalium. Genitourin Med 61, 319–324.
    [Google Scholar]
  27. Taylor-Robinson, D., Gilroy, C. B. & Keane, F. E. ( 2003; ). Detection of several Mycoplasma species at various anatomical sites of homosexual men. Eur J Clin Microbiol Infect Dis 22, 291–293.
    [Google Scholar]
  28. Tully, J. G., Taylor-Robinson, D., Cole, R. M. & Rose, D. L. ( 1981; ). A newly discovered mycoplasma in the human urogenital tract. Lancet 1, 1288–1291.
    [Google Scholar]
  29. Tully, J. G., Rose, D. L., Baseman, J. B., Dallo, S. F., Lazzell, A. L. & Davis, C. P. ( 1995; ). Mycoplasma pneumoniae and Mycoplasma genitalium mixture in synovial fluid isolate. J Clin Microbiol 33, 1851–1855.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.009977-0
Loading
/content/journal/jmm/10.1099/jmm.0.009977-0
Loading

Data & Media loading...

Supplements

vol. , part 7, pp. 895-899

Sequence alignment of the gene of . [PDF](17 KB)



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error