1887

Abstract

Two new -selective agars, from Oxoid (according to Brazier) and from BD, were compared with cycloserine-cefoxitin-fructose agar (Oxoid) for their sensitivity of recovery of toxigenic from stool samples. For the culture-positive samples, the sensitivities were 84.0, 42.6 and 90.4 %, respectively. In addition, a specific fluorescence hybridization assay was developed, facilitating rapid and reliable identification of cultured isolates.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.009811-0
2009-07-01
2019-11-17
Loading full text...

Full text loading...

/deliver/fulltext/jmm/58/7/874.html?itemId=/content/journal/jmm/10.1099/jmm.0.009811-0&mimeType=html&fmt=ahah

References

  1. Amann, R. I., Binder, B. J., Olson, R. J., Chisholm, S. W., Devereux, R. & Stahl, D. A. ( 1990; ). Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol 56, 1919–1925.
    [Google Scholar]
  2. Barbut, F., Delmee, M., Brazier, J. S., Petit, J. C., Poxton, I. R., Rupnik, M., Lalande, V., Schneider, C., Mastrantonio, P. & other authors ( 2003; ). A European survey of diagnostic methods and testing protocols for Clostridium difficile. Clin Microbiol Infect 9, 989–996.[CrossRef]
    [Google Scholar]
  3. Blossom, D. B. & McDonald, L. C. ( 2007; ). The challenges posed by reemerging Clostridium difficile infection. Clin Infect Dis 45, 222–227.[CrossRef]
    [Google Scholar]
  4. Brazier, J. S. ( 1993; ). Role of the laboratory in investigations of Clostridium difficile diarrhea. Clin Infect Dis 16, S228–S233.[CrossRef]
    [Google Scholar]
  5. Brazier, J. S. ( 1998; ). The diagnosis of Clostridium difficile-associated disease. J Antimicrob Chemother 41, 29–40.
    [Google Scholar]
  6. Burckhardt, F., Friedrich, A., Beier, D. & Eckmanns, T. ( 2008; ). Clostridium difficile surveillance trends, Saxony, Germany. Emerg Infect Dis 14, 691–692.[CrossRef]
    [Google Scholar]
  7. Celig, D. M. & Schreckenberger, P. C. ( 1991; ). Clinical evaluation of the RapID-ANA II panel for identification of anaerobic bacteria. J Clin Microbiol 29, 457–462.
    [Google Scholar]
  8. Delmee, M., Van Broeck, J., Simon, A., Janssens, M. & Avesani, V. ( 2005; ). Laboratory diagnosis of Clostridium difficile-associated diarrhoea: a plea for culture. J Med Microbiol 54, 187–191.[CrossRef]
    [Google Scholar]
  9. Fallani, M., Rigottier-Gois, L., Aguilera, M., Bridonneau, C., Collignon, A., Edwards, C. A., Corthier, G. & Dore, J. ( 2006; ). Clostridium difficile and Clostridium perfringens species detected in infant faecal microbiota using 16S rRNA targeted probes. J Microbiol Methods 67, 150–161.[CrossRef]
    [Google Scholar]
  10. Fenner, L., Widmer, A. F., Goy, G., Rudin, S. & Frei, R. ( 2008; ). Rapid and reliable diagnostic algorithm for detection of Clostridium difficile. J Clin Microbiol 46, 328–330.[CrossRef]
    [Google Scholar]
  11. George, W. L., Sutter, V. L., Citron, D. & Finegold, S. M. ( 1979; ). Selective and differential medium for isolation of Clostridium difficile. J Clin Microbiol 9, 214–219.
    [Google Scholar]
  12. Gerding, D. N., Olson, M. M., Peterson, L. R., Teasley, D. G., Gebhard, R. L., Schwartz, M. & Lee, T. J. ( 1986; ). Clostridium difficile-associated diarrhea and colitis in adults. A prospective case-controlled epidemiologic study. Arch Intern Med 146, 95–100.[CrossRef]
    [Google Scholar]
  13. Greisen, K., Loeffelholz, M., Purohit, A. & Leong, D. ( 1994; ). PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. J Clin Microbiol 32, 335–351.
    [Google Scholar]
  14. Karachewski, N. O., Busch, E. L. & Wells, C. L. ( 1985; ). Comparison of PRAS II, RapID ANA, and API 20A systems for identification of anaerobic bacteria. J Clin Microbiol 21, 122–126.
    [Google Scholar]
  15. Kuijper, E. J., Coignard, B. & Tull, P. ( 2006; ). Emergence of Clostridium difficile-associated disease in North America and Europe. Clin Microbiol Infect 12, 2–18.
    [Google Scholar]
  16. Kuijper, E. J., Coignard, B., Brazier, J. S., Suetens, C., Drudy, D., Wiuff, C., Pituch, H., Reichert, P., Schneider, F. & other authors ( 2007; ). Update of Clostridium difficile-associated disease due to PCR ribotype 027 in Europe. Euro Surveill 12, E1–E2.
    [Google Scholar]
  17. Manz, W., Amann, R., Ludwig, W., Wagner, M. & Schleifer, K. H. ( 1992; ). Phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria: problems and solutions. Syst Appl Microbiol 15, 593–600.[CrossRef]
    [Google Scholar]
  18. Marler, L. M., Siders, J. A., Wolters, L. C., Pettigrew, Y., Skitt, B. L. & Allen, S. D. ( 1991; ). Evaluation of the new RapID-ANA II system for the identification of clinical anaerobic isolates. J Clin Microbiol 29, 874–878.
    [Google Scholar]
  19. McDonald, L. C., Killgore, G. E., Thompson, A., Owens, R. C., Kazakova, S. V., Sambol, S. P., Johnson, S. & Gerding, D. N. ( 2005; ). An epidemic, toxin gene-variant strain of Clostridium difficile. N Engl J Med 353, 2433–2441.[CrossRef]
    [Google Scholar]
  20. Milinovich, G. J., Trott, D. J., Burrell, P. C., Croser, E. L., Al Jassim, R. A., Morton, J. M., van Eps, A. W. & Pollitt, C. C. ( 2007; ). Fluorescence in situ hybridization analysis of hindgut bacteria associated with the development of equine laminitis. Environ Microbiol 9, 2090–2100.[CrossRef]
    [Google Scholar]
  21. Mundy, L. S., Shanholtzer, C. J., Willard, K. E., Gerding, D. N. & Peterson, L. R. ( 1995; ). Laboratory detection of Clostridium difficile. A comparison of media and incubation systems. Am J Clin Pathol 103, 52–56.
    [Google Scholar]
  22. Nerandzic, M. M. & Donskey, C. J. ( 2009; ). Effective and reduced-cost modified selective medium for isolation of Clostridium difficile. J Clin Microbiol 47, 397–400.[CrossRef]
    [Google Scholar]
  23. Peterson, L. R., Kelly, P. J. & Nordbrock, H. A. ( 1996; ). Role of culture and toxin detection in laboratory testing for diagnosis of Clostridium difficile-associated diarrhea. Eur J Clin Microbiol Infect Dis 15, 330–336.[CrossRef]
    [Google Scholar]
  24. Reller, M. E., Lema, C. A., Perl, T. M., Cai, M., Ross, T. L., Speck, K. A. & Carroll, K. C. ( 2007; ). Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile. J Clin Microbiol 45, 3601–3605.[CrossRef]
    [Google Scholar]
  25. van den Berg, R. J., Bruijnesteijn van Coppenraet, L. S., Gerritsen, H. J., Endtz, H. P., van der Vorm, E. R. & Kuijper, E. J. ( 2005; ). Prospective multicenter evaluation of a new immunoassay and real-time PCR for rapid diagnosis of Clostridium difficile-associated diarrhea in hospitalized patients. J Clin Microbiol 43, 5338–5340.[CrossRef]
    [Google Scholar]
  26. Wilson, K. H., Kennedy, M. J. & Fekety, F. R. ( 1982; ). Use of sodium taurocholate to enhance spore recovery on a medium selective for Clostridium difficile. J Clin Microbiol 15, 443–446.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.009811-0
Loading
/content/journal/jmm/10.1099/jmm.0.009811-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error