1887

Abstract

Diarrhoea in newborn and weaned piglets is mainly caused by enterotoxigenic (ETEC) with fimbriae F4. To investigate the prevalence of resistance to three fimbrial strains, F4ab, F4ac and F4ad, among Chinese indigenous pigs and Western commercial pigs introduced into China, we determined the ETEC F4 adhesion phenotypes in 292 pure-bred piglets from three Western commercial breeds and 12 Chinese indigenous breeds, and a total of 1093 adult pigs in a White Duroc×Erhualian intercross, by an microscopic adhesion assay. All the Tibet and Lantang pigs and a majority of the Erhualian and Rongchang pigs were resistant (nonadherent) to ETEC F4 whereas all the Laiwu pigs and most of the Jiangquhai and Tongcheng pigs were susceptible (adhesive) to at least one of the F4 strains. Yushan Black pigs were uniformly resistant to F4ab, and Jinhua pigs were predominantly resistant to F4ac. Susceptible and resistant animals were observed in the other breeds, indicating that diarrhoea caused by ETEC F4 could be prevalent in these breeds. This study confirmed the existence of eight previously reported F4 adhesion patterns, and supported the assumption that the three F4 receptors are encoded by distinct loci. Expression of the weakly adherent phenotype was observed in six pure-bred piglets and 90 adult F/F animals, and the inheritance of this phenotype and its correlation with susceptibility to disease are still not known.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.009803-0
2009-08-01
2019-12-16
Loading full text...

Full text loading...

/deliver/fulltext/jmm/58/8/1112.html?itemId=/content/journal/jmm/10.1099/jmm.0.009803-0&mimeType=html&fmt=ahah

References

  1. Baker, D. R., Billey, L. O. & Francis, D. H. ( 1997; ). Distribution of K88 Escherichia coli-adhesive and nonadhesive phenotypes among pigs of four breeds. Vet Microbiol 54, 123–132.[CrossRef]
    [Google Scholar]
  2. Bijlsma, I. G. & Bouw, J. ( 1987; ). Inheritance of K88-mediated adhesion of Escherichia coli to jejunal brush borders in pigs: a genetic analysis. Vet Res Commun 11, 509–518.[CrossRef]
    [Google Scholar]
  3. Bijlsma, I. G., de Nijs, A., van der Meer, C. & Frik, J. F. ( 1982; ). Different pig phenotypes affect adherence of Escherichia coli to jejunal brush borders by K88ab, K88ac and K88ad antigen. Infect Immun 37, 891–894.
    [Google Scholar]
  4. Bonneau, M., Duval-Iflah, Y., Guerin, G., Ollivier, L., Renard, C. & Renjifo, X. ( 1990; ). Aspects genetiques et microbiologiques de la colibacillose K88 chez le porc. Ann Rech Vet 21, 302–303.
    [Google Scholar]
  5. Chandler, D. S., Mynott, T. L., Luke, R. K. & Craven, J. A. ( 1994; ). The distribution and stability of Escherichia coli K88 receptor in the gastrointestinal tract of the pig. Vet Microbiol 38, 203–215.[CrossRef]
    [Google Scholar]
  6. Chappuis, J. P., Duval-Iflah, O., Ollivier, L. & Legault, C. ( 1984; ). Escherichia coli K88 adhesion: a comparison of Chinese and Large White piglets. Genet Sel Evol 16, 385–390.[CrossRef]
    [Google Scholar]
  7. Gibbons, R. A., Sellwood, R., Burrows, M. R. & Hunter, P. A. ( 1977; ). Inheritance of resistance to neonatal E.coli diarrhoea in the pig: examination of the genetic system. Theor Appl Genet 51, 65–70.[CrossRef]
    [Google Scholar]
  8. Guérin, G., Duval-Iflah, Y., Bonneau, M., Bertaud, M., Guillaume, P. & Ollivier, L. ( 1993; ). Evidence for linkage between K88ab, K88ac intestinal receptors to Escherichia coli and transferrin loci in pigs. Anim Genet 24, 393–396.
    [Google Scholar]
  9. Guinée, P. A. M. & Jansen, W. H. ( 1979; ). Behavior of Escherichia coli K antigens K88ab, K88ac, and K88ad in immunoelectrophoresis, double diffusion, and hemagglutination. Infect Immun 23, 700–705.
    [Google Scholar]
  10. Hu, Z. L., Hasler-Rapacz, J., Huang, S. C. & Rapacz, J. ( 1993; ). Studies in swine on inheritance and variation in expression of small intestinal receptors mediating adhesion of the K88 enteropathogenic Escherichia coli variants. J Hered 84, 157–165.
    [Google Scholar]
  11. Jin, L. Z. & Zhao, X. ( 2000; ). Intestinal receptors for adhesive fimbriae of enterotoxigenic Escherichia coli (ETEC) K88 in swine – a review. Appl Microbiol Biotechnol 54, 311–318.[CrossRef]
    [Google Scholar]
  12. Li, Y. H., Qiu, X. T., Li, H. J. & Zhang, Q. ( 2007; ). Adhesive patterns of Escherichia coli F4 in piglets of three breeds. J Genet Genomics 34, 591–599.[CrossRef]
    [Google Scholar]
  13. Michaels, R. D., Whipp, S. C. & Rothschild, M. F. ( 1994; ). Resistance of Chinese Meishan, Fengjing, and Minzhu pigs to the K88ac+ strain of Escherichia coli. Am J Vet Res 55, 333–338.
    [Google Scholar]
  14. Moon, H. W., Hoffman, L. J., Cornick, N. A., Booher, S. L. & Bosworth, B. T. ( 1999; ). Prevalence of some virulence genes among Escherichia coli isolates from swine presented to a diagnostic laboratory in Iowa. J Vet Diagn Invest 11, 557–560.[CrossRef]
    [Google Scholar]
  15. Python, P., Jörg, H., Neuenschwander, S., Hagger, C., Stricker, C., Bürgi, E., Bertschinger, H. U., Stranzinger, G. & Vögeli, P. ( 2002; ). Fine-mapping of the intestinal receptor locus for enterotoxigenic Escherichia coli F4ac on porcine chromosome 13. Anim Genet 33, 441–447.[CrossRef]
    [Google Scholar]
  16. Python, P., Jörg, H., Neuenschwander, S., Asai-Coakwell, M., Hagger, C., Bürgi, E., Bertschinger, H. U., Stranzinger, G. & Vögeli, P. ( 2005; ). Inheritance of the F4ab, F4ac and F4ad E. coli receptors in swine and examination of four candidate genes for F4acR. J Anim Breed Genet 122 (Suppl. 1), 5–14.[CrossRef]
    [Google Scholar]
  17. Rapacz, J. & Hasler-Rapacz, J. ( 1986; ). Polymorphism and inheritance of swine small intestinal receptors mediating adhesion of three serological variants of Escherichia coli-producing K88 pilus antigen. Anim Genet 17, 305–321.
    [Google Scholar]
  18. Rutter, J. M., Burrows, M. R., Sellwood, R. & Gibbons, R. A. ( 1975; ). A genetic basis for resistance to enteric disease caused by E. coli. Nature 257, 135–136.[CrossRef]
    [Google Scholar]
  19. Sellwood, R., Gibbons, R. A., Jones, G. W. & Rutter, J. M. ( 1975; ). Adhesion of enteropathogenic Escherichia coli to pig intestinal brush borders: the existence of two pig phenotypes. J Med Microbiol 8, 405–411.[CrossRef]
    [Google Scholar]
  20. Van den Broeck, W., Cox, E., Oudega, B. & Goddeeris, B. M. ( 2000; ). The F4 fimbrial antigen of Escherichia coli and its receptors. Vet Microbiol 71, 223–244.[CrossRef]
    [Google Scholar]
  21. Zhang, Z. G., Li, B. D. & Chen, X. H. ( 1986; ). Pig Breeds in China. Shanghai: Shanghai Scientific and Technical Publisher.
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.009803-0
Loading
/content/journal/jmm/10.1099/jmm.0.009803-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error