1887

Abstract

biofilm causes device-related meningitis in neurosurgical patients. This study assessed the contribution of polysaccharide and protein to the development of a strong biofilm-positive phenotype in four isolates associated with probable device-related meningitis, under varying environmental conditions. RT-PCR analysis of the intercellular adhesion operon () and assessment of polysaccharide intercellular adhesin (PIA) production indicated a correlation between increased transcription and PIA production in isolates grown in medium with 4 % ethanol and 4 % NaCl. Treatment of biofilm with sodium metaperiodate caused dispersion of adhered cells ( <0.0001), indicating involvement of PIA. Transcriptional levels of protein factors revealed that transcription levels were similar in all isolates, whilst levels were variable, with induction being seen in two isolates following growth in the presence of alcohol or salt. Transcription of did not influence protein expression and transcription varied among the strains. Although transcription was induced, the treatment of biofilm with proteinase K did not always disperse the biofilm. Our data suggest that, among the three isolates clinically associated with meningitis that were studied, PIA contributed to the strong biofilm-positive phenotype, whereas protein factors appeared to have a secondary role.

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2009-07-01
2019-10-23
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