@article{mbs:/content/journal/jmm/10.1099/jmm.0.009175-0, author = "Cerqueira, Gustavo M. and McBride, Alan J. A. and Picardeau, Mathieu and Ribeiro, Samuel G. and Moreira, Ângela N. and Morel, Viviane and Reis, Mitermayer G. and Ko, Albert I. and Dellagostin, Odir A.", title = "Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates", journal= "Journal of Medical Microbiology", year = "2009", volume = "58", number = "9", pages = "1173-1181", doi = "https://doi.org/10.1099/jmm.0.009175-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.009175-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "dN/dS, ratio of non-synonymous to synonymous substitutions", abstract = "The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence of lig genes among serovars from a collection of leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited number of pathogenic serovars, the ligB gene was distributed ubiquitously among all pathogenic strains. None of the lig genes were detected among intermediate or saprophytic Leptospira species. It was also shown that, similar to the previously characterized secY gene, a short specific PCR fragment of ligB could be used to correctly identify pathogenic Leptospira species. These findings demonstrate that ligB is widely present among pathogenic strains and may be useful for their reliable identification and classification.", }