1887

Abstract

Clinical isolates of (=81) from patients with pulmonary infections who were HIV-negative and isolates (=33) from HIV-positive patients were subjected to genetic analysis by PCR detection of three -specific insertion sequences (IS, IS and IS), and nucleotide sequencing of the heat-shock protein 65 () gene. All clinical isolates were identified as ‘ subspecies ’ by sequence analysis of . Compared with clinical isolates of reported elsewhere, IS was found less frequently in Japanese isolates (96/114 isolates, 84 %) and IS was detected more frequently (76/114 isolates, 67 %). One isolate was found to lack IS, which has not been reported previously for ‘ subsp. ’. Nucleotide sequence analysis of the PCR products for IS revealed that all clinical isolates had the same new insertion sequence, designated IS, which had 60 point mutations compared with the nucleotide sequence of the original IS. These results suggest that ‘ subsp. ’ with IS is prevalent in Japan. IS may have implications for the virulence of and contribute to an increase of infections in this country.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.008623-0
2009-07-01
2024-11-10
Loading full text...

Full text loading...

/deliver/fulltext/jmm/58/7/945.html?itemId=/content/journal/jmm/10.1099/jmm.0.008623-0&mimeType=html&fmt=ahah

References

  1. Alvarez J., García I. G., Aranaz A., Bezos J., Romero B., de Juan L., Mateos A., Gómez-Mampaso E., Domínguez L. 2008; Genetic diversity of Mycobacterium avium isolates recovered from clinical samples and from the environment: molecular characterization for diagnostic purposes. J Clin Microbiol 46:1246–1251 [CrossRef]
    [Google Scholar]
  2. Chacon O., Bermudez L. E., Barletta R. G. 2004; Johne's disease, inflammatory bowel disease, and Mycobacterium paratuberculosis . Annu Rev Microbiol 58:329–363 [CrossRef]
    [Google Scholar]
  3. Dvorska L., Bull T. J., Bartos M., Matlova L., Svastova P., Weston R. T., Kintr J., Parmova I., van Soolingen D., Pavlik I. 2003; A standardized restriction fragment length polymorphism (RFLP) method for typing Mycobacterium avium isolates links IS 901 with virulence for birds. J Microbiol Methods 55:11–27 [CrossRef]
    [Google Scholar]
  4. Harris N. B., Barletta R. G. 2001; Mycobacterium avium subsp. paratuberculosis in veterinary medicine. Clin Microbiol Rev 14:489–512 [CrossRef]
    [Google Scholar]
  5. Henry M. T., Inamdar L., O'Riordain D., Schweiger M., Watson J. P. 2004; Nontuberculous mycobacteria in non-HIV patients: epidemiology, treatment and response. Eur Respir J 23:741–746 [CrossRef]
    [Google Scholar]
  6. Johansen T. B., Djonne B., Jensen M. R., Olsen I. 2005; Distribution of IS 1311 and IS 1245 in Mycobacterium avium subspecies revisited. J Clin Microbiol 43:2500–2502 [CrossRef]
    [Google Scholar]
  7. Kunze Z. M., Wall S., Appelberg R., Silva M. T., Portaels F., Mcfadden J. J. 1991; IS 901 , a new member of a widespread class of atypical insertion sequences, is associated with pathogenicity in Mycobacterium avium . Mol Microbiol 5:2265–2272 [CrossRef]
    [Google Scholar]
  8. Kunze Z. M., Portaels F., Mcfadden J. J. 1992; Biologically distinct subtypes of Mycobacterium avium differ in possession of insertion sequence IS 901 . J Clin Microbiol 30:2366–2372
    [Google Scholar]
  9. Kuwabara K., Watanabe Y., Wada K., Tsuchiya T. 2004; Relations between clinical subtypes of Mycobacterium avium pulmonary disease and polyclonal infections detected by IS 1245 based restriction fragment length polymorphism analysis. Kekkaku 79:39–46 (in Japanese
    [Google Scholar]
  10. Marras T. K., Daley C. L. 2002; Epidemiology of human pulmonary infection with nontuberculous mycobacteria. Clin Chest Med 23:553–567 [CrossRef]
    [Google Scholar]
  11. Mijs W., de Haas P., Rossau R., Van der Laan T., Rigouts L., Portaels F., van Soolingen D. 2002; Molecular evidence to support a proposal to reserve the designation Mycobacterium avium subsp. avium for bird-type isolates and ‘ M.avium subsp. hominissuis ’ for the human/porcine type of M. avium . Int J Syst Evol Microbiol 52:1505–1518 [CrossRef]
    [Google Scholar]
  12. Möbius P., Lentzsch P., Moser I., Naumann L., Martin G., Köhler H. 2006; Comparative macrorestriction and RFLP analysis of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. hominissuis isolates from man, pig, and cattle. Vet Microbiol 117:284–291 [CrossRef]
    [Google Scholar]
  13. Moriyama M., Ogawa K., Nishimori K., Uchiya K., Ito T., Yagi T., Nakashima I., Nakagawa T., Tarumi O., Nikai T. 2006; Usefulness of variable numbers of tandem repeats typing in clinical strains of Mycobacterium avium . Kekkaku 81:559–566 (in Japanese
    [Google Scholar]
  14. Nishimori K., Eguchi M., Nakaoka Y., Onodera Y., Ito T., Tanaka K. 1995; Distribution of IS 901 in strains of Mycobacterium avium complex from swine by using IS 901 -detecting primers that discriminate between M. avium and Mycobacterium intracellulare . J Clin Microbiol 33:2102–2106
    [Google Scholar]
  15. Pavlik I., Svastova P., Bartl J., Dvorska L., Rychlik I. 2000; Relationship between IS 901 in the Mycobacterium avium complex strains isolated from birds, animals, humans, and the environment and virulence for poultry. Clin Diagn Lab Immunol 7:212–217
    [Google Scholar]
  16. Ritacco V., Kremer K., Van der Laan T., Pijnenburg J. E., de Haas P. E., van Soolingen D. 1998; Use of IS 901 and IS 1245 in RFLP typing of Mycobacterium avium complex: relatedness among serovar reference strains, human and animal isolates. Int J Tuberc Lung Dis 2:242–251
    [Google Scholar]
  17. Sakatani M. 1999; Nontuberculous mycobacteriosis: the present status of epidemiology and clinical studies. Kekkaku 74:377–384 (in Japanese
    [Google Scholar]
  18. Thierry D., Vincent V., Clément F., Guesdon J. L. 1993; Isolation of specific DNA fragments of Mycobacterium avium and their possible use in diagnosis. J Clin Microbiol 31:1048–1054
    [Google Scholar]
  19. Turenne C. Y., Semret M., Cousins D. V., Collins D. M., Behr M. A. 2006; Sequencing of hsp65 distinguishes among subsets of the Mycobacterium avium complex. J Clin Microbiol 44:433–440 [CrossRef]
    [Google Scholar]
  20. Turenne C. Y., Wallace R. Jr, Behr M. A. 2007; Mycobacterium avium in the postgenomic era. Clin Microbiol Rev 20:205–229 [CrossRef]
    [Google Scholar]
/content/journal/jmm/10.1099/jmm.0.008623-0
Loading
/content/journal/jmm/10.1099/jmm.0.008623-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error