RT Journal Article SR Electronic(1) A1 Bhowmick, Tushar Suvra A1 Das, Mayukh A1 Ruppitsch, Werner A1 Stoeger, Anna A1 Pietzka, Ariane Tatjana A1 Allerberger, Franz A1 Rodrigues, D. P. A1 Sarkar, B. L.YR 2009 T1 Detection of virulence-associated and regulatory protein genes in association with phage typing of human Vibrio cholerae from several geographical regions of the world JF Journal of Medical Microbiology, VO 58 IS 9 SP 1160 OP 1167 DO https://doi.org/10.1099/jmm.0.008466-0 PB Microbiology Society, SN 1473-5644, AB Vibrio cholerae O1, O139 and occasionally non-O1/non-O139 serogroups are most often responsible for epidemic and pandemic cholera. This study used genotypic patterns of PCR-based detection of virulence-associated and regulatory protein genes, along with phage typing, to characterize 86 V. cholerae strains. Thirty-eight of 53 O1 biotype El Tor strains harboured both tcpA classical and tcpA El Tor genes, and three El Tor strains lacked the V. cholerae O1-specific gene (Vc-O1); three O139 strains contained both Vc-O1 and Vc-O139 genes and seven out of ten non-O1/non-O139 strains possessed the Vc-O1 gene. The latter strains all harboured the virulence-associated genes ctxA, zot, ace, RS1, hlyA, ompU, rtxA and sxt. Two phage types, T27 and T25, were predominant in strains from different geographical regions of India, whereas more variation in phage susceptibility was observed for tetracycline-resistant strains from Kolkata. These results suggest that the pattern and distribution of virulence genes and phage types of V. cholerae are equally useful and discriminatory in tracing the origin of newly emerging strains., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.008466-0