1887

Abstract

O1, O139 and occasionally non-O1/non-O139 serogroups are most often responsible for epidemic and pandemic cholera. This study used genotypic patterns of PCR-based detection of virulence-associated and regulatory protein genes, along with phage typing, to characterize 86 strains. Thirty-eight of 53 O1 biotype El Tor strains harboured both classical and El Tor genes, and three El Tor strains lacked the O1-specific gene (); three O139 strains contained both and genes and seven out of ten non-O1/non-O139 strains possessed the gene. The latter strains all harboured the virulence-associated genes , , , , , , and . Two phage types, T27 and T25, were predominant in strains from different geographical regions of India, whereas more variation in phage susceptibility was observed for tetracycline-resistant strains from Kolkata. These results suggest that the pattern and distribution of virulence genes and phage types of are equally useful and discriminatory in tracing the origin of newly emerging strains.

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2009-09-01
2019-10-23
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