1887

Abstract

To identify the time frame and route of mother-to-child infection, a Mongolian gerbil model was used. Four-week-old female Mongolian gerbils were infected with , and then mated with uninfected males 2 months after infection. The offspring were sacrificed weekly after birth, and then serum, mother's milk from the stomach and gastric tissues were obtained from pups. Anti- antibody titres were measured in sera and maternal milk using an ELISA. The stomach was cut in two in the sagittal plane, and then colonization in mucosa was confirmed by culture and real-time RT-PCR in one specimen and by immunochemical staining in the other. Faeces and oral swabs were obtained from infected mothers, and 16S rRNA was measured using real-time RT-PCR. was not identified in cultures from the gastric mucosa of pups delivered by infected mothers, but 16S rRNA was detected from 4 weeks after birth, suggesting that Mongolian gerbil pups become infected via maternal transmission from 4 weeks of age. The anti- antibody titre in sera of pups from infected mothers was maximum at 3 weeks of age and then rapidly decreased from 4 weeks of age. High antibody titres in mother's milk were detected during the suckling period, and GlcNAc was detectable at 2–4 weeks of age, but disappeared as the offspring aged. Thus seems to infect Mongolian gerbil pups from 4 weeks of age, in parallel with decreasing GlcNAc expression in the gastric mucosa. These results suggested that infection of Mongolian gerbil pups occurs via faecal–oral transmission from an infected mother.

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2009-05-01
2024-12-12
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References

  1. Atherton J. C., Cao P., Peek R. M., Tummuru M. K. R., Blaser M. J., Cover T. L. 1995; Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori . J Biol Chem 270:17771–17777 [CrossRef]
    [Google Scholar]
  2. Bamford K. B., Bickley J., Collins J. S. A., Johnston B. T., Potts S., Boston V., Owen R. J., Sloan J. M. 1993; Helicobacter pylori : comparison of DNA fingerprints provides evidence for intrafamilial infection. Gut 34:1348–1350 [CrossRef]
    [Google Scholar]
  3. Blecker U., McKeithan T. W., Hart J., Kirschner B. S. 1995; Resolution of Helicobacter pylori- associated gastric lymphoproliferative disease in a child. Gastroenterology 109:973–977 [CrossRef]
    [Google Scholar]
  4. Brenner H., Bode G., Adler G., Rothenbacher D. 2000; Does maternal smoking hinder mother-child transmission of Helicobacter pylori infection?. Epidemiology 11:71–75 [CrossRef]
    [Google Scholar]
  5. Covacci A., Telford J. L., Del Giudice G., Parsonnet J., Rappuoli R. 1999; Helicobacter pylori virulence and genetic geography. Science 284:1328–1333 [CrossRef]
    [Google Scholar]
  6. Drumm B., Perez-Perez G. I., Blaser M. J., Sherman P. M. 1990; Intrafamilial clustering of Helicobacter pylori infection. N Engl J Med 322:359–363 [CrossRef]
    [Google Scholar]
  7. Engstrand L., Nguyen A. M., Graham D. Y., El-Saatari F. A. 1992; Reverse transcription and polymerase chain reaction amplification of rRNA for detection of Helicobacter species. J Clin Microbiol 30:2295–2301
    [Google Scholar]
  8. Goodwin C. S. 1997; Helicobacter pylori gastritis, peptic ulcer, and gastric cancer: clinical and molecular aspects. Clin Infect Dis 25:1017–1019 [CrossRef]
    [Google Scholar]
  9. Granstrom M., Tindberg Y., Blennow M. 1997; Seroepidemiology of Helicobacter pylori infection in a cohort of children monitored from 6 months to 11 years of age. J Clin Microbiol 35:468–470
    [Google Scholar]
  10. Horn C. C., Ciucci M., Chaudhury A. 2007; Brain Fos expression during 48 h after cisplatin treatment: neural pathways for acute and delayed visceral sickness. Auton Neurosci 132:44–51 [CrossRef]
    [Google Scholar]
  11. IARC Working Group 1994; Schistosomes, liver flukes and Helicobacter pylori . IARC Monogr Eval Carcinog Risks Hum 61:177–241
    [Google Scholar]
  12. Karita M., Matsumoto S., Kamei T., Shinohara K., Sugiyama T. 2005; Direct transmission of H. pylori from challenged to nonchallenged mice in a single cage. Dig Dis Sci 50:1092–1096 [CrossRef]
    [Google Scholar]
  13. Kawakubo M., Ito Y., Okimura Y., Kobayashi M., Sakura K., Kasama S., Fukuda M. N., Fukuda M., Karsuyama T., Nakayama J. 2004; Natural antibiotic function of a human gastric mucin against Helicobacter pylori infection. Science 305:1003–1006 [CrossRef]
    [Google Scholar]
  14. Kivi M., Tindberg Y., Sörberg M., Casswall T. H., Befrits R., Hellström P. M., Bengtsson C., Engstrand L., Granström M. 2003; Concordance of Helicobacter pylori strains within families. J Clin Microbiol 41:5604–5608 [CrossRef]
    [Google Scholar]
  15. Konno M., Fujii N., Yokota S., Sato K., Takahashi M., Sato K., Mino E., Sugiyama T. 2005; Five-year follow-up study of mother-to-child transmission of Helicobacter pylori infection detected by a random amplified polymorphic DNA fingerprinting method. J Clin Microbiol 43:2246–2250 [CrossRef]
    [Google Scholar]
  16. Kuss H., Hoefgen N., Johanssen S., Kronbach T., Rundfeldti C. 2003; In vivo efficacy in airway disease models of N -(3,5-dichloropyrid-4-yl)-[1-(4-fluorobenzyl)-5-hydroxy-indole-3-yl]-glyoxylic acid amide (AWD 12-281), a selective phosphodiesterase 4 inhibitor for inhaled administration. J Pharmacol Exp Ther 307:373–385 [CrossRef]
    [Google Scholar]
  17. Malaty H. M., Graham D. Y., Klein P. D., Evans D. G., Adam E., Evans D. J. 1991; Transmission of Helicobacter pylori infection. Studies in families of healthy individuals. Scand J Gastroenterol 26:927–932 [CrossRef]
    [Google Scholar]
  18. Malaty H. M., Graham D. Y., Wattigney W. A., Srinivasan S. R., Osato M., Berenson G. S. 1999; Natural history of Helicobacter pylori infection in childhood: 12-year follow-up cohort study in a biracial community. Clin Infect Dis 28:279–282 [CrossRef]
    [Google Scholar]
  19. Marshall B. J., Warren J. R. 1984; Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1:1311–1315
    [Google Scholar]
  20. Matsumoto S., Washizuka Y., Matsumoto Y., Tawara S., Ikeda F., Yokota Y., Karita M. 1997; Induction of ulceration and severe gastritis in Mongolian gerbil by Helicobacter pylori infection. J Med Microbiol 46:391–397 [CrossRef]
    [Google Scholar]
  21. Minoura T., Kato S., Otsu S., Kodama M., Fujioka T., Iinuma K., Nishizono A. 2005; Influence of age and duration of infection on bacterial load and immune responses to Helicobacter pylori infection in a murine model. Clin Exp Immunol 139:43–47 [CrossRef]
    [Google Scholar]
  22. Nakagawa S., Osaki T., Fujioka Y., Yamaguchi H., Kamiya S. 2005; Long-term infection of mongolian gerbils with Helicobacter pylori : microbiological, histopathological, and serological analyses. Clin Diagn Lab Immunol 12:347–353
    [Google Scholar]
  23. NIH Consensus Conference 1994; Helicobacter pylori in peptic ulcer disease. JAMA 272:65–68 [CrossRef]
    [Google Scholar]
  24. Nwokolo C. U., Bickley J., Attard A. R., Owen R. J., Costas M., Fraser I. A. 1992; Evidence of clonal variants of Helicobacter pylori in three generations of a duodenal ulcer disease family. Gut 33:1323–1327 [CrossRef]
    [Google Scholar]
  25. Oderda G., Vaira D., Holton J., Ainley C., Altare F., Boero M., Smith A., Ansaldi N. 1991; Helicobacter pylori in children with peptic ulcer and their families. Dig Dis Sci 36:572–576 [CrossRef]
    [Google Scholar]
  26. Osaki T., Hanawa T., Manzoku T., Fukuda M., Kawakami H., Suzuki H., Yamaguchi H., Yan X., Taguchi H. other authors 2006; Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of a Mongolian gerbil model. J Med Microbiol 55:1477–1485 [CrossRef]
    [Google Scholar]
  27. Parsonnet J., Shmuely H., Haggerty T. 1999; Fecal and oral shedding of Helicobacter pylori from healthy infected adults. JAMA 282:2240–2245 [CrossRef]
    [Google Scholar]
  28. Rothenbacher D., Bode G., Berg G., Knayer U., Gonser T., Adler G., Brenner H. 1999; Helicobacter pylori among preschool children and their parents: evidence of parent-child transmission. J Infect Dis 179:398–402 [CrossRef]
    [Google Scholar]
  29. Rothenbacher D., Bode G., Brenner H. 2000; Helicobacter pylori among siblings. Lancet 355: 1998 [CrossRef]
    [Google Scholar]
  30. Rowland M. 2000; Transmission of Helicobacter pylori : is it all child's play?. Lancet 355:332–333 [CrossRef]
    [Google Scholar]
  31. Sarker S. A., Mahalanabis D., Hildebrand P., Rahaman M. M., Bardhan P. K., Fuchs G., Beglinger C., Gyr K. 1997; Helicobacter pylori : prevalence, transmission, and serum pepsinogen II concentrations in children of a poor periurban community in Bangladesh. Clin Infect Dis 25:990–995 [CrossRef]
    [Google Scholar]
  32. Sawada Y., Yamamoto N., Sakagami T., Fukuda Y., Shimoyama T., Nishigami T., Uematsu K., Nakagawa K. 1999; Comparison of pathologic changes in Helicobacter pylori -induced Mongolian gerbils and humans. J Gastroenterol 34:55–60
    [Google Scholar]
  33. Taylor N. S., Fox J. G., Akopyants N. S., Berg D. E., Thompson N., Shames B., Yan L., Fontham E., Janney F., Hunter F. M. 1995; Long-term colonization with single and multiple strains of Helicobacter pylori assessed by DNA fingerprinting. J Clin Microbiol 33:918–923
    [Google Scholar]
  34. Warren J. R., Marshall B. 1983; Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 1:1273–1275
    [Google Scholar]
  35. Watanabe T., Tada M., Nagai H., Sasaki S., Nakao M. 1998; Helicobacter pylori infection induces gastric cancer in Mongolian gerbils. Gastroenterology 115:642–648 [CrossRef]
    [Google Scholar]
  36. Weyermann M., Adler G., Brenner H., Rothenbacher D. 2006; The mother as source of Helicobacter pylori infection. Epidemiology 17:332–334 [CrossRef]
    [Google Scholar]
  37. Yoshimatsu T., Shirai M., Nagata K., Okita K., Nakazawa T. 2000; Transmission of Helicobacter pylori from challenged to nonchallenged nude mice kept in a single cage. Dig Dis Sci 45:1747–1753 [CrossRef]
    [Google Scholar]
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