1887

Abstract

To identify the time frame and route of mother-to-child infection, a Mongolian gerbil model was used. Four-week-old female Mongolian gerbils were infected with , and then mated with uninfected males 2 months after infection. The offspring were sacrificed weekly after birth, and then serum, mother's milk from the stomach and gastric tissues were obtained from pups. Anti- antibody titres were measured in sera and maternal milk using an ELISA. The stomach was cut in two in the sagittal plane, and then colonization in mucosa was confirmed by culture and real-time RT-PCR in one specimen and by immunochemical staining in the other. Faeces and oral swabs were obtained from infected mothers, and 16S rRNA was measured using real-time RT-PCR. was not identified in cultures from the gastric mucosa of pups delivered by infected mothers, but 16S rRNA was detected from 4 weeks after birth, suggesting that Mongolian gerbil pups become infected via maternal transmission from 4 weeks of age. The anti- antibody titre in sera of pups from infected mothers was maximum at 3 weeks of age and then rapidly decreased from 4 weeks of age. High antibody titres in mother's milk were detected during the suckling period, and GlcNAc was detectable at 2–4 weeks of age, but disappeared as the offspring aged. Thus seems to infect Mongolian gerbil pups from 4 weeks of age, in parallel with decreasing GlcNAc expression in the gastric mucosa. These results suggested that infection of Mongolian gerbil pups occurs via faecal–oral transmission from an infected mother.

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2009-05-01
2019-10-14
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