%0 Journal Article %A Tapsall, John %A Read, Phillip %A Carmody, Christopher %A Bourne, Christopher %A Ray, Sanghamitra %A Limnios, Athena %A Sloots, Theo %A Whiley, David %T Two cases of failed ceftriaxone treatment in pharyngeal gonorrhoea verified by molecular microbiological methods %D 2009 %J Journal of Medical Microbiology, %V 58 %N 5 %P 683-687 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.007641-0 %K IM, intramuscular %K FVU, first void urine %K NG-MAST, Neisseria gonorrhoeae multi-antigen sequence typing %K NAAT, nucleic acid amplification test %K FAM, 6-carboxyfluorescein %K BHQ, black-hole quencher %I Microbiology Society, %X Diagnostic, genotypic and antibiotic-resistance determinants of Neisseria gonorrhoeae were analysed by molecular methods to verify the failure of ceftriaxone treatment in two cases of pharyngeal gonorrhoea. Monoplex assays were needed to define competitive inhibition of a positive Chlamydia PCR in a duplex assay. Different penA changes were detected in the N. gonorrhoeae isolated from the two cases. These were associated with raised ceftriaxone MICs of 0.03 and 0.016 mg l−1, which may have contributed to the treatment failures in these cases. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.007641-0