RT Journal Article SR Electronic(1) A1 Matějková, Petra A1 Flasarová, Magdalena A1 Zákoucká, Hana A1 Bořek, Milan A1 Křemenová, Soňa A1 Arenberger, Petr A1 Woznicová, Vladana A1 Weinstock, George M. A1 Šmajs, DavidYR 2009 T1 Macrolide treatment failure in a case of secondary syphilis: a novel A2059G mutation in the 23S rRNA gene of Treponema pallidum subsp. pallidum JF Journal of Medical Microbiology, VO 58 IS 6 SP 832 OP 836 DO https://doi.org/10.1099/jmm.0.007542-0 PB Microbiology Society, SN 1473-5644, AB We report an occurrence of treatment failure after oral spiramycin therapy in a man with secondary syphilis and a reported penicillin and tetracycline allergy. Molecular detection revealed treponemal DNA in the blood of the patient and sequencing of the 23S rDNA identified an A to G transition at the gene position corresponding to position 2059 in the Escherichia coli 23S rRNA gene. The occurrence of this novel 23S rDNA mutation was examined among 7 rabbit-propagated syphilitic strains of Treponema pallidum and among 22 syphilis patient isolates from the Czech Republic. The prevalence of A2058G and A2059G mutations among clinical specimens was 18.2 and 18.2 %, respectively., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.007542-0