%0 Journal Article %A Lee, Andie S. %A Jelfs, Peter %A Sintchenko, Vitali %A Gilbert, Gwendolyn L. %T Identification of non-tuberculous mycobacteria: utility of the GenoType Mycobacterium CM/AS assay compared with HPLC and 16S rRNA gene sequencing %D 2009 %J Journal of Medical Microbiology, %V 58 %N 7 %P 900-904 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.007484-0 %K ITS, intergenic transcribed spacer %K NTM, non-tuberculous mycobacteria %I Microbiology Society, %X Non-tuberculous mycobacteria (NTM) causing clinical disease have become increasingly common and more diverse. A new reverse line probe assay, GenoType Mycobacterium CM/AS (Hain Lifescience), was evaluated for identification of a broad range of NTM. It was compared with phenotypic (HPLC) and molecular (DNA probes, in-house real-time multiplex species-specific PCR, 16S rRNA gene PCR and sequencing) identification techniques, which together provided the reference ‘gold standard’. A total of 131 clinical isolates belonging to 31 Mycobacterium species and 19 controls, including 5 non-Mycobacterium species, was used. Concordant results between the GenoType Mycobacterium assay and the reference identification were obtained in 119/131 clinical isolates (90.8 %). Identification of Mycobacterium abscessus and Mycobacterium lentiflavum by the assay was problematic. The GenoType Mycobacterium assay enables rapid identification of a broad range of potentially clinically significant Mycobacterium species, but some species require further testing to differentiate or confirm ambiguous results. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.007484-0