1887

Abstract

Non-tuberculous mycobacteria (NTM) causing clinical disease have become increasingly common and more diverse. A new reverse line probe assay, GenoType Mycobacterium CM/AS (Hain Lifescience), was evaluated for identification of a broad range of NTM. It was compared with phenotypic (HPLC) and molecular (DNA probes, in-house real-time multiplex species-specific PCR, 16S rRNA gene PCR and sequencing) identification techniques, which together provided the reference ‘gold standard’. A total of 131 clinical isolates belonging to 31 species and 19 controls, including 5 non- species, was used. Concordant results between the GenoType Mycobacterium assay and the reference identification were obtained in 119/131 clinical isolates (90.8 %). Identification of and by the assay was problematic. The GenoType Mycobacterium assay enables rapid identification of a broad range of potentially clinically significant species, but some species require further testing to differentiate or confirm ambiguous results.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.007484-0
2009-07-01
2024-12-13
Loading full text...

Full text loading...

/deliver/fulltext/jmm/58/7/900.html?itemId=/content/journal/jmm/10.1099/jmm.0.007484-0&mimeType=html&fmt=ahah

References

  1. Brown-Elliott B. A., Griffith D. E., Wallace R. J. Jr 2002; Newly described or emerging human species of nontuberculous mycobacteria. Infect Dis Clin North Am 16:187–220 [CrossRef]
    [Google Scholar]
  2. Butler W. R., Jost K. C. Jr, Kilburn J. O. 1991; Identification of mycobacteria by high-performance liquid chromatography. J Clin Microbiol 29:2468–2472
    [Google Scholar]
  3. CDC 1996 Standardized Method for HPLC Identification of Mycobacteria Atlanta, GA: Centers for Disease Control and Prevention;
    [Google Scholar]
  4. Devulder G., Pérouse de Montclos M., Flandrois J. P. 2005; A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model. Int J Syst Evol Microbiol 55:293–302 [CrossRef]
    [Google Scholar]
  5. Heifets L. 2004; Mycobacterial infections caused by nontuberculous mycobacteria. Semin Respir Crit Care Med 25:283–295 [CrossRef]
    [Google Scholar]
  6. Legrand E., Sola C., Verdol B., Rastogi N. 2000; Genetic diversity of Mycobacterium avium recovered from AIDS patients in the Caribbean as studied by a consensus IS1245-RFLP method and pulsed-field gel electrophoresis. Res Microbiol 151:271–283 [CrossRef]
    [Google Scholar]
  7. Mäkinen J., Sarkola A., Marjamäki M., Viljanen M. K., Soini H. 2002; Evaluation of GenoType and LiPA MYCOBACTERIA assays for identification of Finnish mycobacterial isolates. J Clin Microbiol 40:3478–3481 [CrossRef]
    [Google Scholar]
  8. Mäkinen J., Marjamäki M., Marttila H., Soini H. 2006; Evaluation of a novel strip test, GenoType Mycobacterium CM/AS, for species identification of mycobacterial cultures. Clin Microbiol Infect 12:481–483 [CrossRef]
    [Google Scholar]
  9. Padilla E., González V., Manterola J. M., Perez A., Quesada M. D., Gordillo S., Vilaplana C., Pallares M. A., Molinos S. other authors 2004; Comparative evaluation of the new version of the INNO-LiPA Mycobacteria and GenoType Mycobacterium assays for identification of Mycobacterium species from MB/BacT liquid cultures artificially inoculated with mycobacterial strains. J Clin Microbiol 42:3083–3088 [CrossRef]
    [Google Scholar]
  10. Relman D. A. 1993; Universal bacterial 16S rRNA amplification and sequencing.. In Diagnostic Molecular Microbiology, Principles and Applications pp 489–495 Edited by Persing D. H., Smith T. F., Tenover F. C., White T. J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  11. Roth A., Fischer M., Hamid M. E., Michalke S., Ludwig W., Mauch H. 1998; Differentiation of phylogenetically related slowly growing mycobacteria based on 16S–23S rRNA gene internal transcribed spacer sequences. J Clin Microbiol 36:139–147
    [Google Scholar]
  12. Ruiz P., Gutierrez J., Zerolo F. J., Casal M. 2002; GenoType Mycobacterium assay for identification of mycobacterial species isolated from human clinical samples by using liquid medium. J Clin Microbiol 40:3076–3078 [CrossRef]
    [Google Scholar]
  13. Russo C., Tortoli E., Menichella D. 2006; Evaluation of the new GenoType Mycobacterium assay for identification of mycobacterial species. J Clin Microbiol 44:334–339 [CrossRef]
    [Google Scholar]
  14. Sarkola A., Mäkinen J., Marjamäki M., Marttila H. J., Viljanen M. K., Soini H. 2004; Prospective evaluation of the GenoType assay for routine identification of mycobacteria. Eur J Clin Microbiol Infect Dis 23:642–645
    [Google Scholar]
  15. Tortoli E. 2003; Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. Clin Microbiol Rev 16:319–354 [CrossRef]
    [Google Scholar]
  16. Xiong L., Kong F., Yang Y., Cheng J., Gilbert G. L. 2006; Use of PCR and reverse line blot hybridization macroarray based on 16S–23S rRNA gene internal transcribed spacer sequences for rapid identification of 34 Mycobacterium species. J Clin Microbiol 44:3544–3550 [CrossRef]
    [Google Scholar]
/content/journal/jmm/10.1099/jmm.0.007484-0
Loading
/content/journal/jmm/10.1099/jmm.0.007484-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error