1887

Abstract

This study established an experimental model of replicative infection in A/J mice. The animals were infected by intratracheal inoculation of 10–10 c.f.u. serogroup 1 (USA clinical isolates D4968, D4969 and D4973). The inocula of 10, 10, 10 and 10 c.f.u. of all tested serogroup 1 isolates were lethal for A/J mice. Inoculation of 10 c.f.u. caused death in 90 % of the animals within 5 days, whilst inoculation of 10 c.f.u. caused sporadic death of mice. All animals that received 10 c.f.u. bacteria developed acute lower respiratory disease, but were able to clear from the lungs within 3 weeks. The kinetics of bacterial growth in the lungs was independent of inoculum size and reached a growth peak about 3 logarithms above the initial inoculum at 72 h after inoculation. The most prominent histological changes in the lungs were observed at 48–72 h after inoculation in the form of a focal, neutrophil-dominant, peribronchiolar infiltration. The inflammatory process did not progress towards the interstitial or alveolar spaces. Immunohistological analyses revealed serogroup 1 during the early phase of infection near the bronchiolar epithelia and later co-localized with inflammatory cells. BALB/c and C57BL/6 mice strains were also susceptible to infection with all serogroup 1 strains tested and very similar changes were observed in the lungs of infected animals. These results underline the infection potential of serogroup 1, which is associated with high morbidity and lethality in mice.

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2009-06-01
2019-12-14
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References

  1. Asare, R. & Abu Kwaik, Y. ( 2007; ). Early trafficking and intracellular replication of Legionella longbeachaea within an ER-derived late endosome-like phagosome. Cell Microbiol 9, 1571–1587.[CrossRef]
    [Google Scholar]
  2. Asare, R., Santic, M., Gobin, I., Doric, M., Suttles, J., Graham, J. E., Price, C. D. & Abu Kwaik, Y. ( 2007; ). Genetic susceptibility and caspase activation in mouse and human macrophages are distinct for Legionella longbeachae and L. pneumophila. Infect Immun 75, 1933–1945.[CrossRef]
    [Google Scholar]
  3. Bibb, W. F., Sorg, R. J., Thomason, B. M., Hicklin, M. D., Steigerwalt, A. G., Brenner, D. J. & Wulf, M. R. ( 1981; ). Recognition of a second serogroup of Legionella longbeachae. J Clin Microbiol 14, 674–677.
    [Google Scholar]
  4. Brieland, J., Freeman, P., Kunkel, R., Chrisp, C., Hurley, M., Fantone, J. & Engleberg, C. ( 1994; ). Replicative Legionella pneumophila lung infection in intratracheally inoculated A/J mice. A murine model of human Legionnaires' disease. Am J Pathol 145, 1537–1546.
    [Google Scholar]
  5. Cameron, S., Roder, D., Walker, C. & Feldheim, J. ( 1991; ). Epidemiological characteristics of Legionella infection in South Australia: implications for disease control. Aust N Z J Med 21, 65–70.[CrossRef]
    [Google Scholar]
  6. CDC ( 2000; ). Legionnaires' disease associated with potting soil – California, Oregon and Washington. MMWR Morb Mortal Wkly Rep 49, 777–778.
    [Google Scholar]
  7. Cianciotto, N. P., Eisenstein, B. I., Mody, C. H. & Engleberg, N. C. ( 1990; ). A mutation in the mip gene results in an attenuation of Legionella pneumophila virulence. J Infect Dis 162, 121–126.[CrossRef]
    [Google Scholar]
  8. Doyle, R. M. & Heuzenroeder, M. W. ( 2002; ). A mutation in an ompR-like gene on a Legionella longbeachae serogroup 1 plasmid attenuates virulence. Int J Med Microbiol 292, 227–239.[CrossRef]
    [Google Scholar]
  9. Doyle, R. M., Cianciotto, N. P., Banvi, S., Manning, P. A. & Heuzenroeder, M. W. ( 2001; ). Comparison of virulence of Legionella longbeachae strains in guinea pigs and U937 macrophage-like cells. Infect Immun 69, 5335–5344.[CrossRef]
    [Google Scholar]
  10. Hughes, M. S. & Steele, T. W. ( 1994; ). Occurrence and distribution of Legionella species in composted plant materials. Appl Environ Microbiol 60, 2003–2005.
    [Google Scholar]
  11. Izu, K., Yoshida, S., Miyamoto, H., Chang, B., Ogawa, M., Yamamoto, H., Goto, Y. & Taniguchi, H. ( 1999; ). Grouping of 20 reference strains of Legionella species by the growth ability within mouse and guinea pig macrophages. FEMS Immunol Med Microbiol 26, 61–68.[CrossRef]
    [Google Scholar]
  12. Koide, M., Saito, A., Okazaki, M., Umeda, B. & Benson, R. F. ( 1999; ). Isolation of Legionella longbeachae serogroup 1 from potting soils in Japan. Clin Infect Dis 29, 943–944.[CrossRef]
    [Google Scholar]
  13. Kümpers, P., Tiede, A., Kirschner, P., Girke, J., Ganser, A. & Peest, D. ( 2008; ). Legionnaires' disease in immunocompromised patients: a case report of Legionella longbeachae pneumonia and review of the literature. J Med Microbiol 57, 384–387.[CrossRef]
    [Google Scholar]
  14. Lanser, J. A., Adams, M., Doyle, R., Sangster, N. & Steele, T. W. ( 1990; ). Genetic relatedness of Legionella longbeachae isolates from human and environmental sources in Australia. Appl Environ Microbiol 56, 2784–2790.
    [Google Scholar]
  15. Lim, I., Sangster, N., Reid, D. P. & Lanser, J. A. ( 1989; ). Legionella longbeachae pneumonia, report of two cases. Med J Aust 150, 599–601.
    [Google Scholar]
  16. McKinney, R. M., Porschen, R. K., Edelstein, P. H., Bissett, M. L., Harris, P. P., Bondell, S. P., Steigerwalt, A. G., Weaver, R. E., Ein, M. E. & other authors ( 1981; ). Legionella longbeachae species nova, another etiologic agent of human pneumonia. Ann Intern Med 94, 739–743.[CrossRef]
    [Google Scholar]
  17. Molofsky, A. B., Byrne, B. G., Whitfield, N. N., Madigan, C. A., Fuse, E. T., Tateda, K. & Swanson, M. S. ( 2006; ). Cytosolic recognition of flagellin by mouse macrophages restricts Legionella pneumophila infection. J Exp Med 203, 1093–1104.[CrossRef]
    [Google Scholar]
  18. Neumeister, B., Schöniger, S., Faigle, M., Eichner, M. & Deitz, K. ( 1997; ). Multiplication of different Legionella species in Mono Mac 6 cells and in Acanthamoeba castellanii. Appl Environ Microbiol 63, 1219–1224.
    [Google Scholar]
  19. O'Connor, B. A., Carman, J., Eckert, K., Tucker, G., Givney, R. & Cameron, S. ( 2007; ). Does using potting mix make you sick? Results from a Legionella longbeachae case–control study in South Australia. Epidemiol Infect 135, 34–39.[CrossRef]
    [Google Scholar]
  20. Phares, C. R., Wangroongsarb, P., Chantra, S., Paveenkitiporn, W., Tondella, M. L., Benson, R. F., Thacker, W. L., Fields, B. S., Moore, M. R. & other authors ( 2007; ). Epidemiology of severe pneumonia caused by Legionella longbeachae, Mycoplasma pneumoniae, and Chlamydia pneumoniae: 1-year, population-based surveillance for severe pneumonia in Thailand. Clin Infect Dis 45, e147–e155.[CrossRef]
    [Google Scholar]
  21. Steele, T. W., Lanser, J. & Sangster, N. ( 1990; ). Isolation of Legionella longbeachae serogroup 1 from potting mixes. Appl Environ Microbiol 56, 49–53.
    [Google Scholar]
  22. Susa, M., Ticac, B., Rukavina, T., Doric, M. & Marre, R. ( 1998; ). Legionella pneumophila infection in intratracheally inoculated T cell-depleted or -nondepleted A/J mice. J Immunol 160, 316–321.
    [Google Scholar]
  23. Wadowsky, R. M., Wilson, T. M., Kapp, N. J., West, A. J., Kuchta, J. M., States, S. J., Dowling, J. N. & Yee, R. B. ( 1991; ). Multiplication of Legionella spp. in tap water containing Hartmannella vermiformis. Appl Environ Microbiol 57, 1950–1955.
    [Google Scholar]
  24. Wright, E. K., Goodart, S. A., Growney, J. D., Hadinoto, V., Endrizzi, M. G., Long, E. M., Sadigh, K., Abney, A. L., Bernstein-Hanley, I. & Dietrich, W. F. ( 2003; ). Naip5 affects host susceptibility to the intracellular pathogen Legionella pneumophila. Curr Biol 13, 27–36.[CrossRef]
    [Google Scholar]
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