1887

Abstract

The emergence of carbapenem-hydrolysing metallo--lactamases (MBLs) is a serious threat to the clinical utility of carbapenems. This study identified plasmid- and integron-borne and in clinical isolates of . The and gene cassettes were carried by a class 1 integron and followed by the gene cassette. The and gene cassettes were preceded by a weak P promoter, TGGACA(N)TAAGCT, and an inactive P2 promoter, TTGTTA(N)TACAGT. These genes were easily transferred to by conjugation and transformation, indicating that they are located on transferable plasmids. Due to the acquisition of , the susceptibility of transconjugants to imipenem, meropenem, panipenem and biapenem decreased by 32-, 256-, 64- and 128-fold, respectively. In comparison, after gaining , the susceptibility of transconjugants to the four carbapenems decreased by 64-, 2048-, 256- and 64-fold, respectively. Strains harbouring showed higher-level resistance to imipenem, meropenem and panipenem than the strains harbouring , although the nucleotide sequences of the class 1 integrons carrying and were identical except for a single point mutation.

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2009-02-01
2019-10-18
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