1887

Abstract

The emergence of carbapenem-hydrolysing metallo--lactamases (MBLs) is a serious threat to the clinical utility of carbapenems. This study identified plasmid- and integron-borne and in clinical isolates of . The and gene cassettes were carried by a class 1 integron and followed by the gene cassette. The and gene cassettes were preceded by a weak P promoter, TGGACA(N)TAAGCT, and an inactive P2 promoter, TTGTTA(N)TACAGT. These genes were easily transferred to by conjugation and transformation, indicating that they are located on transferable plasmids. Due to the acquisition of , the susceptibility of transconjugants to imipenem, meropenem, panipenem and biapenem decreased by 32-, 256-, 64- and 128-fold, respectively. In comparison, after gaining , the susceptibility of transconjugants to the four carbapenems decreased by 64-, 2048-, 256- and 64-fold, respectively. Strains harbouring showed higher-level resistance to imipenem, meropenem and panipenem than the strains harbouring , although the nucleotide sequences of the class 1 integrons carrying and were identical except for a single point mutation.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.006874-0
2009-02-01
2021-04-22
Loading full text...

Full text loading...

/deliver/fulltext/jmm/58/2/217.html?itemId=/content/journal/jmm/10.1099/jmm.0.006874-0&mimeType=html&fmt=ahah

References

  1. Arakawa Y., Murakami M., Suzuki K., Ito H., Wacharotayankun R., Ohsuka S., Kato N., Ohta M. 1995; A novel integron-like element carrying the metallo- β -lactamase gene bla IMP . Antimicrob Agents Chemother 39:1612–1615 [CrossRef]
    [Google Scholar]
  2. Arakawa Y., Shibata N., Shibayama K., Kurokawa H., Yagi T., Fujiwara H., Goto M. 2000; Convenient test for screening metallo- β -lactamase-producing Gram-negative bacteria by using thiol compounds. J Clin Microbiol 38:40–43
    [Google Scholar]
  3. Bennett P. M. 1999; Integrons and gene cassettes: a genetic construction kit for bacteria. J Antimicrob Chemother 43:1–4
    [Google Scholar]
  4. Bunny K. L., Hall R. M., Stokes H. W. 1995; New mobile gene cassettes containing an aminoglycoside resistance gene, aacA7 , and a chloramphenicol resistance gene, catB3 , in an integron in pBWH301. Antimicrob Agents Chemother 39:686–693 [CrossRef]
    [Google Scholar]
  5. Bush K. 1998; Metallo- β -lactamases: a class apart. Clin Infect Dis 27 (Suppl. 1):S48–S53 [CrossRef]
    [Google Scholar]
  6. Collis C. M., Hall R. M. 1995; Expression of antibiotic resistance genes in the integrated cassettes of integrons. Antimicrob Agents Chemother 39:155–162 [CrossRef]
    [Google Scholar]
  7. Galani I., Souli M., Chryssouli Z., Orlandou K., Giamarellou H. 2005; Characterization of a new integron containing bla VIM-1 and aac(6’)-IIc in an Enterobacter cloacae clinical isolate from Greece. J Antimicrob Chemother 55:634–638 [CrossRef]
    [Google Scholar]
  8. Gilbert D. N., Moellering R. C., Eliopoulos G. M., Sande M. A. 2005 The Sanford Guide to Antimicrobial Therapy 2005 , 35th edn. Hyde Park, VT: Antimicrobial Therapy;
    [Google Scholar]
  9. Hall R. M., Collis C. M. 1995; Mobile gene cassettes and integrons: capture and spread of genes by site-specific recombination. Mol Microbiol 15:593–600
    [Google Scholar]
  10. Ito H., Arakawa Y., Ohsuka S., Wacharotayankun R., Kato N., Ohta M. 1995; Plasmid-mediated dissemination of the metallo- β -lactamase gene bla IMP among clinically isolated strains of Serratia marcescens . Antimicrob Agents Chemother 39:824–829 [CrossRef]
    [Google Scholar]
  11. Iyobe S., Kusadokoro H., Takahashi A., Yomoda S., Okubo T., Nakamura A., O'Hara K. 2002; Detection of a variant metallo- β -lactamase, IMP-10, from two unrelated strains of Pseudomonas aeruginosa and an Alcaligenes xylosoxidans strain. Antimicrob Agents Chemother 46:2014–2016 [CrossRef]
    [Google Scholar]
  12. Lahey Clinic 2008; Amino acid sequences for TEM, SHV and OXA extended-spectrum and inhibitor resistant β -lactamases. (accessed 24 September 2008 http://www.lahey.org/Studies
  13. Lévesque C., Brassard S., Lapointe J., Roy P. H. 1994; Diversity and relative strength of tandem promoters for the antibiotic-resistance genes of several integrons. Gene 142:49–54 [CrossRef]
    [Google Scholar]
  14. Livermore D. M., Woodford N. 2000; Carbapenemases: a problem in waiting?. Curr Opin Microbiol 3:489–495 [CrossRef]
    [Google Scholar]
  15. Mazel D. 2006; Integrons: agents of bacterial evolution. Nat Rev Microbiol 4:608–620 [CrossRef]
    [Google Scholar]
  16. NCCLS 2000 Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically , 5th edn. Document M7-A5 Wayne, PA: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  17. Nordmann P., Poirel L. 2002; Emerging carbapenemases in Gram-negative aerobes. Clin Microbiol Infect 8:321–331 [CrossRef]
    [Google Scholar]
  18. Osano E., Arakawa Y., Wacharotayankun R., Ohta M., Horii T., Ito H., Yoshimura F., Kato N. 1994; Molecular characterization of an enterobacterial metallo β -lactamase found in a clinical isolate of Serratia marcescens that shows imipenem resistance. Antimicrob Agents Chemother 38:71–78 [CrossRef]
    [Google Scholar]
  19. Shibata N., Doi Y., Yamane K., Yagi T., Kurokawa H., Shibayama K., Kato H., Kai K., Arakawa Y. 2003; PCR typing of genetic determinants for metallo- β -lactamases and integrases carried by Gram-negative bacteria isolated in Japan, with focus on the class 3 integron. J Clin Microbiol 41:5407–5413 [CrossRef]
    [Google Scholar]
  20. Stokes H. W., Hall R. M. 1989; A novel family of potentially mobile DNA elements encoding site-specific gene-integration functions: integrons. Mol Microbiol 3:1669–1683 [CrossRef]
    [Google Scholar]
  21. Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B. 1995; Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33:2233–2239
    [Google Scholar]
  22. Walsh T. R., Toleman M. A., Poirel L., Nordmann P. 2005; Metallo- β -lactamases: the quiet before the storm?. Clin Microbiol Rev 18:306–325 [CrossRef]
    [Google Scholar]
  23. Zhao W.-H., Hu Z.-Q., Shimamura T. 2008; Potency of IMP-10 metallo- β -lactamase in hydrolysing various antipseudomonal β -lactams. J Med Microbiol 57:974–979 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.006874-0
Loading
/content/journal/jmm/10.1099/jmm.0.006874-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error