1887

Abstract

A rare case of a severe prosthetic joint infection in a 71-year-old immunocompetent woman is presented. was identified in two consecutive samples using broad-range PCR and sequencing, whereas cultivation remained negative for the first sample and streptococci of a non-group A streptococci, non-group B streptococci type were detected for the second one. This report demonstrates that the phenotypic approach may lead to misidentification of in a routine clinical setting. Molecular methods of pathogen detection might be useful when a rare and/or unexpected micro-organism is present or the sample is collected during antibiotic treatment.

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2009-06-01
2019-10-14
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References

  1. Anguita-Alonso, P., Hanssen, A. D. & Patel, R. ( 2005; ). Prosthetic joint infection. Expert Rev Anti Infect Ther 3, 797–804.[CrossRef]
    [Google Scholar]
  2. Bille, J., Rocourt, J. & Swamingnathan, B. ( 2003; ). Listeria and Erysipelothrix. In Manual of Clinical Microbiology, 8th edn, pp. 461–471. Edited by P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller & R. H. Yolken. Washington, DC: American Society for Microbiology.
  3. Cone, L. A., Somero, M. S., Qureshi, F. J., Kerkar, S., Byrd, R. G., Hirschberg, J. M. & Gauto, A. R. ( 2008; ). Unusual infections due to Listeria monocytogenes in the Southern California desert. Int J Infect Dis 12, 578–581.[CrossRef]
    [Google Scholar]
  4. Corless, C. E., Guiver, M., Borrow, R., Edwards-Jones, V., Kaczmarski, E. B. & Fox, A. J. ( 2000; ). Contamination and sensitivity issues with real-time universal 16S rRNA PCR. J Clin Microbiol 38, 1747–1752.
    [Google Scholar]
  5. Drancourt, M., Bollet, C., Carlioz, A., Martelin, R., Gayral, J.-P. & Raoult, D. ( 2000; ). 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical bacterial isolates. J Clin Microbiol 38, 3623–3630.
    [Google Scholar]
  6. Fenollar, F., Roux, V., Stein, A., Drancourt, M. & Raoult, D. ( 2006; ). Analysis of 525 samples to determine the usefulness of PCR amplification and sequencing of the 16S rRNA gene for diagnosis of bone and joint infections. J Clin Microbiol 44, 1018–1028.[CrossRef]
    [Google Scholar]
  7. Gallo, J., Bauer, P., Denis, M., Libečková, Y., Kolář, M., Zapletalová, J. & Janout, V. ( 2006; ). Molecular diagnostics for the detection of prosthetic joint infection. Acta Chir Orthop Traumatol Cech 73, 85–91.
    [Google Scholar]
  8. Gómez-Rodríguez, N., Ibánez Ruán, J. & González Pérez, M. ( 2006; ). Prosthetic knee infection caused by Listeria monocytogenes in a woman with rheumatoid arthritis and Waldenström's macroglobulinemia. An Med Interna 23, 276–278 (in Spanish).
    [Google Scholar]
  9. Handrick, W., Schwede, I., Tomalik, T. & Berthold, F. ( 2008; ). Arthritis due to Listeria monocytogenes. Z Rheumatol 67, 68–71.[CrossRef]
    [Google Scholar]
  10. Kesteman, T., Yombi, J.-C., Gigi, J. & Durez, P. ( 2007; ). Listeria infections associated with infliximab: case reports. Clin Rheumatol 26, 2173–2175.[CrossRef]
    [Google Scholar]
  11. Marculescu, C. E., Berbari, E. F., Cockerill, F. R., III & Osmon, D. R. ( 2006; ). Unusual aerobic and anaerobic bacteria associated with prosthetic joint infections. Clin Orthop Relat Res 451, 55–63.[CrossRef]
    [Google Scholar]
  12. Petti, C. A., Polage, C. R. & Schreckenberger, P. ( 2005; ). The role of 16S rRNA gene sequencing in identification of microorganisms misidentified by conventional methods. J Clin Microbiol 43, 6123–6125.[CrossRef]
    [Google Scholar]
  13. Rantakokko-Jalava, K., Nikkari, S., Jalava, J., Eerola, E., Skurnik, M., Meurman, O., Ruuskanen, O., Alanen, A., Kotilainen, E. & other authors ( 2000; ). Direct amplification of rRNA genes in diagnosis of bacterial infections. J Clin Microbiol 38, 32–39.
    [Google Scholar]
  14. Schett, G., Herak, P., Graninger, W., Smolen, J. S. & Aringer, M. ( 2005; ). Listeria-associated arthritis in a patient undergoing etanercept therapy: case report and review of the literature. J Clin Microbiol 43, 2537–2541.[CrossRef]
    [Google Scholar]
  15. Schuchat, A., Swaminathan, B. & Broome, C. V. ( 1991; ). Epidemiology of human listeriosis. Clin Microbiol Rev 4, 169–183.
    [Google Scholar]
  16. Tien, C. H., Huang, G. S., Chang, C. C., Chang, D. M. & Lai, J. H. ( 2008; ). Acupuncture-associated Listeria monocytogenes arthritis in a patient with rheumatoid arthritis. Joint Bone Spine 75, 502–503.[CrossRef]
    [Google Scholar]
  17. Wilkinson, N. Z., Kingsley, G. H., Jones, H. W., Sieper, J., Braun, J. & Ward, M. E. ( 1999; ). The detection of DNA from a range of bacterial species in the joints of patients with a variety of arthritides using a nested, broad-range polymerase chain reaction. Rheumatology 38, 260–266.[CrossRef]
    [Google Scholar]
  18. Zimmerli, W. ( 2006; ). Prosthetic-joint-associated infections. Best Pract Res Clin Rheumatol 20, 1045–1063.[CrossRef]
    [Google Scholar]
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