1887

Abstract

A rare case of a severe prosthetic joint infection in a 71-year-old immunocompetent woman is presented. was identified in two consecutive samples using broad-range PCR and sequencing, whereas cultivation remained negative for the first sample and streptococci of a non-group A streptococci, non-group B streptococci type were detected for the second one. This report demonstrates that the phenotypic approach may lead to misidentification of in a routine clinical setting. Molecular methods of pathogen detection might be useful when a rare and/or unexpected micro-organism is present or the sample is collected during antibiotic treatment.

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2009-06-01
2020-04-10
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References

  1. Anguita-Alonso P., Hanssen A. D., Patel R. 2005; Prosthetic joint infection. Expert Rev Anti Infect Ther 3:797–804 [CrossRef]
    [Google Scholar]
  2. Bille J., Rocourt J., Swamingnathan B. 2003; Listeria and Erysipelothrix . In Manual of Clinical Microbiology , 8th edn. pp 461–471 Edited by Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  3. Cone L. A., Somero M. S., Qureshi F. J., Kerkar S., Byrd R. G., Hirschberg J. M., Gauto A. R. 2008; Unusual infections due to Listeria monocytogenes in the Southern California desert. Int J Infect Dis 12:578–581 [CrossRef]
    [Google Scholar]
  4. Corless C. E., Guiver M., Borrow R., Edwards-Jones V., Kaczmarski E. B., Fox A. J. 2000; Contamination and sensitivity issues with real-time universal 16S rRNA PCR. J Clin Microbiol 38:1747–1752
    [Google Scholar]
  5. Drancourt M., Bollet C., Carlioz A., Martelin R., Gayral J.-P., Raoult D. 2000; 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical bacterial isolates. J Clin Microbiol 38:3623–3630
    [Google Scholar]
  6. Fenollar F., Roux V., Stein A., Drancourt M., Raoult D. 2006; Analysis of 525 samples to determine the usefulness of PCR amplification and sequencing of the 16S rRNA gene for diagnosis of bone and joint infections. J Clin Microbiol 44:1018–1028 [CrossRef]
    [Google Scholar]
  7. Gallo J., Bauer P., Denis M., Libečková Y., Kolář M., Zapletalová J., Janout V. 2006; Molecular diagnostics for the detection of prosthetic joint infection. Acta Chir Orthop Traumatol Cech 73:85–91
    [Google Scholar]
  8. Gómez-Rodríguez N., Ibánez Ruán J., González Pérez M. 2006; Prosthetic knee infection caused by Listeria monocytogenes in a woman with rheumatoid arthritis and Waldenström's macroglobulinemia. An Med Interna 23:276–278 (in Spanish)
    [Google Scholar]
  9. Handrick W., Schwede I., Tomalik T., Berthold F. 2008; Arthritis due to Listeria monocytogenes . Z Rheumatol 67:68–71 [CrossRef]
    [Google Scholar]
  10. Kesteman T., Yombi J.-C., Gigi J., Durez P. 2007; Listeria infections associated with infliximab: case reports. Clin Rheumatol 26:2173–2175 [CrossRef]
    [Google Scholar]
  11. Marculescu C. E., Berbari E. F., Cockerill F. R. III, Osmon D. R. 2006; Unusual aerobic and anaerobic bacteria associated with prosthetic joint infections. Clin Orthop Relat Res 451:55–63 [CrossRef]
    [Google Scholar]
  12. Petti C. A., Polage C. R., Schreckenberger P. 2005; The role of 16S rRNA gene sequencing in identification of microorganisms misidentified by conventional methods. J Clin Microbiol 43:6123–6125 [CrossRef]
    [Google Scholar]
  13. Rantakokko-Jalava K., Nikkari S., Jalava J., Eerola E., Skurnik M., Meurman O., Ruuskanen O., Alanen A., Kotilainen E. other authors 2000; Direct amplification of rRNA genes in diagnosis of bacterial infections. J Clin Microbiol 38:32–39
    [Google Scholar]
  14. Schett G., Herak P., Graninger W., Smolen J. S., Aringer M. 2005; Listeria -associated arthritis in a patient undergoing etanercept therapy: case report and review of the literature. J Clin Microbiol 43:2537–2541 [CrossRef]
    [Google Scholar]
  15. Schuchat A., Swaminathan B., Broome C. V. 1991; Epidemiology of human listeriosis. Clin Microbiol Rev 4:169–183
    [Google Scholar]
  16. Tien C. H., Huang G. S., Chang C. C., Chang D. M., Lai J. H. 2008; Acupuncture-associated Listeria monocytogenes arthritis in a patient with rheumatoid arthritis. Joint Bone Spine 75:502–503 [CrossRef]
    [Google Scholar]
  17. Wilkinson N. Z., Kingsley G. H., Jones H. W., Sieper J., Braun J., Ward M. E. 1999; The detection of DNA from a range of bacterial species in the joints of patients with a variety of arthritides using a nested, broad-range polymerase chain reaction. Rheumatology 38:260–266 [CrossRef]
    [Google Scholar]
  18. Zimmerli W. 2006; Prosthetic-joint-associated infections. Best Pract Res Clin Rheumatol 20:1045–1063 [CrossRef]
    [Google Scholar]
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