1887

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Volume 58, Issue 5

Virulence spectra of typed strains of from different sources: a blinded study Free

Abstract

is a major cause of human diarrhoeal disease, but specific virulence mechanisms have not been well defined. The aims of the present blinded study were to measure and compare the properties of 40 serotyped, biotyped and genotyped isolates from different sources and genetic makeup. An 11-day-old chick embryo lethality assay, which measured embryo deaths and total viable bacteria over 72 h following inoculation of bacteria into the chorioallantoic membrane, revealed a spectrum of activity within the strains. Human and chicken isolates showed similar high virulence values for embryo deaths while the virulence of the bovine isolates was less pronounced. A one-way ANOVA comparison between the capacity of the strains to kill the chick embryos after 24 h with cytotoxicity towards cultured CaCo-2 cells was significant (=0.025). After inoculation with a strain, mouse ligated ileal loops were examined histologically and revealed degrees of villous atrophy, abnormal mucosa, dilation of the lumen, congestion and blood in lumen, depending on the isolate examined. A ‘total pathology score’, derived for each strain after grading the pathology features for degree of severity, showed no apparent relationship with the source of isolation. Some relationship was found between amplified fragment length polymorphism groups and total ileal loop pathology scores, and a one-way ANOVA comparison of the mouse pathology scores against total chick embryo deaths after 72 h was significant (=0.049).

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Keyword(s): AFLP, amplified fragment length polymorphism
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2009-05-01
2024-04-18
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References

  1. Bakhiet M., Al-Salloom F. S., Qareiballa A., Bindayna K., Farid I., Botta G. A. 2004; Induction of alpha and beta chemokines by intestinal epithelial cells stimulated with Campylobacter jejuni . J Infect 48:236–244 [CrossRef]
     [Google Scholar]
  2. Black R. E., Perlman D., Clements M. L., Levine M. M., Blaser M. J. 1992; Human volunteer studies with Campylobacter jejuni . In Campylobacter jejuni : Current Status and Future Trends pp 207–215 Edited by Nachamkin I., Blaser M. J., Tomkins I. S. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  3. Blaser M. J., Duncan D. J., Warren G. H., Wang W.-L. L. 1983; Experimental Campylobacter jejuni infection in adult mice. Infect Immun 39:908–916
     [Google Scholar]
  4. Butzler J. P., Skirrow M. B. 1979; Campylobacter enteritis. Clin Gastroenterol 8:737–765
     [Google Scholar]
  5. Coote J. G., Stewart-Tull D. E. S., Owen R. J., Bolton F. J., Siemer B. L., Candlish D., Thompson D. H., Wardlaw A. C., On S. L. other authors 2007; Comparison of virulence-associated in vitro properties of typed strains of Campylobacter jejuni from different sources. J Med Microbiol 56:722–732 [CrossRef]
     [Google Scholar]
  6. Everest P. H., Goosens H., Sibbons P., Lloyd D. R., Knutton S., Leece R., Ketley J. M., Williams P. H. 1993; Pathological changes in the rabbit ileal loop model caused by Campylobacter jejuni from human colitis. J Med Microbiol 38:316–321 [CrossRef]
     [Google Scholar]
  7. Field L. H., Headley V. L., Underwood J. L., Payne S. M., Berry L. B. 1986; The chicken embryo as a model for Campylobacter invasion: comparative virulence of human isolates of Campylobacter jejuni and Campylobacter coli . Infect Immun 54:118–125
     [Google Scholar]
  8. Fitzgerald C., Owen R. J., Stanley J. 1996; Comprehensive ribotyping scheme for heat-stable serotypes of Campylobacter jejuni . J Clin Microbiol 34:265–269
     [Google Scholar]
  9. Hickey T. E., Baqar S., Bourgeois A. L., Ewing C. P., Guerry P. 1999; Campylobacter jejuni stimulated secretion of interleukin-8 by INT407 cells. Infect Immun 67:88–93
     [Google Scholar]
  10. Hickey T. E., McVeigh A. L., Scott D. A., Michielutti R. E., Bixby A., Carroll S. A., Bourgeois A. L., Guerry P. 2000; Campylobacter jejuni cytolethal distending toxin mediates release of interleukin-8 by intestinal cells. Infect Immun 68:6535–6541 [CrossRef]
     [Google Scholar]
  11. Hu L., Hickey T. L. 2005; Campylobacter jejuni induces secretion of proinflammatory chemokines from human intestinal epithelial cells. Infect Immun 73:4437–4440 [CrossRef]
     [Google Scholar]
  12. MacCallum A., Hardy S. P., Everest P. H. 2005; Campylobacter jejuni inhibits the absorptive transport functions of Caco-2 cells and disrupts cellular tight junctions. Microbiology 151:2451–2458 [CrossRef]
     [Google Scholar]
  13. MacCallum A. J., Harris D., Haddock G., Everest P. H. 2006; Campylobacter jejuni - infected human epithelial cell lines vary in their ability to secrete interleukin-8 compared to in vitro -infected primary human intestinal tissue. Microbiology 152:3661–3665 [CrossRef]
     [Google Scholar]
  14. Newell D. G. 1984; Experimental studies of Campylobacter enteritis . In Campylobacter Infections in Man and Animals . pp 113–131 Edited by Butzler J. P. Boca Raton, FL: CRC Press;
  15. Newell D. G. 2001; Animal models of Campylobacter jejuni colonization and disease and the lessons to be learned from similar Helicobacter pylori models. Symp Ser Soc Appl Microbiol57S–67S
     [Google Scholar]
  16. On S. L. W., Harrington C. S. 2000; Identification of taxonomic and epidemiological relationships among Campylobacter species by numerical analysis of AFLP profiles. FEMS Microbiol Lett 193:161–169 [CrossRef]
     [Google Scholar]
  17. Owen R. J., Sutherland K., Fitzgerald C., Gibson J., Borman P., Stanley J. 1995; Molecular subtyping scheme for serotypes HS1 and HS4 of Campylobacter jejuni . J Clin Microbiol 33:872–877
     [Google Scholar]
  18. Siemer B. L., Harrington C. S., Nielsen E. M., Borck B., Nielsen N. L., Engberg J., On S. L. W. 2004; Genetic relatedness among Campylobacter jejuni serotyped isolates of diverse origin as determined by numerical analysis of amplified fragment length polymorphism (AFLP) profiles. J Appl Microbiol 96:795–802 [CrossRef]
     [Google Scholar]
  19. Stewart-Tull D. E. S., Lucas C., Bleakley C. R. 2004; Experimental immunization with cholera toxoid and mucinase enzymes and protection after challenge with cholera toxin or live virulent Vibrio cholerae . Vaccine 22:2137–2145 [CrossRef]
     [Google Scholar]
  20. van Spreeuwel J. P., Duursma G. C., Meijer C. J., Bax R., Rosekrans P. C., Lindeman J. 1985; Campylobacter colitis: histological, immunohistochemical and ultrastructural findings. Gut 26:945–951 [CrossRef]
     [Google Scholar]
  21. Walker R. I., Rollons D. M., Burr D. H. 1992; Studies of Campylobacter infection in the adult rabbit. In Campylobacter jejuni : Current Status and Future Trends . pp 139–147 Edited by Nachamkin I., Blaser M. J., Tomkins I. S. Washington, DC: American Society for Microbiology;
  22. Wardlaw A. C. 2000 Practical Statistics for Experimental Biologists , 2nd edn. Chichester: Wiley;
     [Google Scholar]
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Virulence spectra of typed strains of Campylobacter jejuni from different sources: a blinded in vivo study
J. Med. Microbiol. 58, 546 (2009); https://doi.org/10.1099/jmm.0.005611-0
/content/journal/jmm/10.1099/jmm.0.005611-0
/content/journal/jmm/10.1099/jmm.0.005611-0
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