1887

Abstract

The FnBPA and FnBPB proteins promote acid-induced biofilm accumulation. Meticillin-resistant (MRSA) isolates from device-related infections with both and produced significantly more biofilm than isolates with either gene alone. Under mildly acidic growth conditions, FnBP-mediated biofilm and and transcript levels were substantially higher during growth at 37 °C than at 30 °C. Thus, in addition to a lowered pH, carriage of both and and growth at 37 °C promote MRSA biofilm development, further supporting a role for the FnBPA and FnBPB surface proteins in the pathogenesis of MRSA device-related infections.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.005504-0
2009-04-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jmm/58/4/399.html?itemId=/content/journal/jmm/10.1099/jmm.0.005504-0&mimeType=html&fmt=ahah

References

  1. Christensen, G. D., Simpson, W. A., Younger, J. J., Baddour, L. M., Barrett, F. F., Melton, D. M. & Beachey, E. H. ( 1985; ). Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol 22, 996–1006.
    [Google Scholar]
  2. Fitzpatrick, F., Humphreys, H. & O'Gara, J. P. ( 2005; ). Evidence for icaADBC-independent biofilm development mechanism in methicillin-resistant Staphylococcus aureus clinical isolates. J Clin Microbiol 43, 1973–1976.[CrossRef]
    [Google Scholar]
  3. Foster, T. J. & Hook, M. ( 1998; ). Surface protein adhesins of Staphylococcus aureus. Trends Microbiol 6, 484–488.[CrossRef]
    [Google Scholar]
  4. Goerke, C., Campana, S., Bayer, M. G., Doring, G., Botzenhart, K. & Wolz, C. ( 2000; ). Direct quantitative transcript analysis of the agr regulon of Staphylococcus aureus during human infection in comparison to the expression profile in vitro. Infect Immun 68, 1304–1311.[CrossRef]
    [Google Scholar]
  5. Montdargent, B. & Letourneur, D. ( 2000; ). Toward new biomaterials. Infect Control Hosp Epidemiol 21, 404–410.[CrossRef]
    [Google Scholar]
  6. O'Gara, J. P. ( 2007; ). ica and beyond: biofilm mechanisms and regulation in Staphylococcus epidermidis and Staphylococcus aureus. FEMS Microbiol Lett 270, 179–188.[CrossRef]
    [Google Scholar]
  7. O'Grady, N. P. ( 2002; ). Applying the science to the prevention of catheter-related infections. J Crit Care 17, 114–121.[CrossRef]
    [Google Scholar]
  8. O'Neill, E., Pozzi, C., Houston, P., Smyth, D., Humphreys, H., Robinson, D. A. & O'Gara, J. P. ( 2007; ). Association between methicillin susceptibility and biofilm regulation in Staphylococcus aureus isolates from device-related infections. J Clin Microbiol 45, 1379–1388.[CrossRef]
    [Google Scholar]
  9. O'Neill, E., Pozzi, C., Houston, P., Humphreys, H., Robinson, D. A., Loughman, A., Foster, T. J. & O'Gara, J. P. ( 2008; ). A novel Staphylococcus aureus biofilm phenotype mediated by the fibronectin-binding proteins, FnBPA and FnBPB. J Bacteriol 190, 3835–3850.[CrossRef]
    [Google Scholar]
  10. Ziebuhr, W., Heilmann, C., Gotz, F., Meyer, P., Wilms, K., Straube, E. & Hacker, J. ( 1997; ). Detection of the intercellular adhesion gene cluster (ica) and phase variation in Staphylococcus epidermidis blood culture strains and mucosal isolates. Infect Immun 65, 890–896.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.005504-0
Loading
/content/journal/jmm/10.1099/jmm.0.005504-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error