1887

Abstract

, the causative agent of cholera, has the ability to grow and survive in the aquatic free-living amoeba . The aim of the present study was to examine the ability of the clinical isolate O139 MO10 to grow in and to determine the effect of the bacterial capsule and LPS O side chain on intracellular growth. Results from co-cultivation, viable counts, a gentamicin assay, electron microscopy and statistical analysis showed that the association of O139 MO10 with did not inhibit growth of the amoeba, and enhanced growth and survival of O139 MO10 occurred. The wild-type O139 MO10 and a capsule mutant or capsule/LPS double mutant grew inside . Neither the capsule nor the LPS O side chain of O139 was found to play an important role in the interaction with , disclosing the ability of to multiply and survive inside , as well as the role of as an environmental host for .

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2009-01-01
2019-10-15
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References

  1. Abd, H., Johansson, T., Golovliov, I., Sandstrom, G. & Forsman, M. ( 2003; ). Survival and growth of Francisella tularensis in Acanthamoeba castellanii. Appl Environ Microbiol 69, 600–606.[CrossRef]
    [Google Scholar]
  2. Abd, H., Weintraub, A. & Sandstrom, G. ( 2005; ). Intracellular survival and replication of Vibrio cholerae O139 in aquatic free-living amoebae. Environ Microbiol 7, 1003–1008.[CrossRef]
    [Google Scholar]
  3. Abd, H., Saeed, A., Weintraub, A., Nair, G. B. & Sandstrom, G. ( 2007; ). Vibrio cholerae O1 strains are facultative intracellular bacteria, able to survive and multiply symbiotically inside the aquatic free-living amoeba Acanthamoeba castellanii. FEMS Microbiol Ecol 60, 33–39.[CrossRef]
    [Google Scholar]
  4. Alam, M., Miyoshi, S., Tomochika, K. & Shinoda, S. ( 1997; ). Hemagglutination is a novel biological function of lipopolysaccharide (LPS), as seen with the Vibrio cholerae O139 LPS. Clin Diagn Lab Immunol 4, 604–606.
    [Google Scholar]
  5. Albert, M. J., Qadri, F., Bhuiyan, N. A., Ahmad, S. M., Ansaruzzaman, M. & Weintraub, A. ( 1999; ). Phagocytosis of Vibrio cholerae O139 Bengal by human polymorphonuclear leukocytes. Clin Diagn Lab Immunol 6, 276–278.
    [Google Scholar]
  6. Alsam, S., Jeong, S. R., Sissons, J., Dudley, R., Kim, K. S. & Khan, N. A. ( 2006; ). Escherichia coli interactions with Acanthamoeba: a symbiosis with environmental and clinical implications. J Med Microbiol 55, 689–694.[CrossRef]
    [Google Scholar]
  7. Axelsson-Olsson, D., Waldenstrom, J., Broman, T., Olsen, B. & Holmberg, M. ( 2005; ). Protozoan Acanthamoeba polyphaga as a potential reservoir for Campylobacter jejuni. Appl Environ Microbiol 71, 987–992.[CrossRef]
    [Google Scholar]
  8. Backer, H. ( 2002; ). Water disinfection for international and wilderness travelers. Clin Infect Dis 34, 355–364.[CrossRef]
    [Google Scholar]
  9. Barker, J., Humphrey, T. J. & Brown, M. W. ( 1999; ). Survival of Escherichia coli O157 in a soil protozoan: implications for disease. FEMS Microbiol Lett 173, 291–295.[CrossRef]
    [Google Scholar]
  10. Behets, J., Declerck, P., Delaedt, Y., Verelst, L. & Ollevier, F. ( 2007; ). Survey for the presence of specific free-living amoebae in cooling waters from Belgian power plants. Parasitol Res 100, 1249–1256.[CrossRef]
    [Google Scholar]
  11. Brown, M. R. & Barker, J. ( 1999; ). Unexplored reservoirs of pathogenic bacteria: protozoa and biofilms. Trends Microbiol 7, 46–50.[CrossRef]
    [Google Scholar]
  12. Chiavelli, D. A., Marsh, J. W. & Taylor, R. K. ( 2001; ). The mannose-sensitive hemagglutinin of Vibrio cholerae promotes adherence to zooplankton. Appl Environ Microbiol 67, 3220–3225.[CrossRef]
    [Google Scholar]
  13. Cirillo, J. D., Falkow, S. & Tompkins, L. S. ( 1994; ). Growth of Legionella pneumophila in Acanthamoeba castellanii enhances invasion. Infect Immun 62, 3254–3261.
    [Google Scholar]
  14. Dearborn, D. G. & Korn, E. D. ( 1974; ). Lipophosphonoglycan of the plasma membrane of Acanthamoeba castellanii. Fatty acid composition. J Biol Chem 249, 3342–3346.
    [Google Scholar]
  15. Faruque, S. M., Naser, I. B., Islam, M. J., Faruque, A. S., Ghosh, A. N., Nair, G. B., Sack, D. A. & Mekalanos, J. J. ( 2005; ). Seasonal epidemics of cholera inversely correlate with the prevalence of environmental cholera phages. Proc Natl Acad Sci U S A 102, 1702–1707.[CrossRef]
    [Google Scholar]
  16. Gaze, W. H., Burroughs, N., Gallagher, M. P. & Wellington, E. M. ( 2003; ). Interactions between Salmonella typhimurium and Acanthamoeba polyphaga, and observation of a new mode of intracellular growth within contractile vacuoles. Microb Ecol 46, 358–369.[CrossRef]
    [Google Scholar]
  17. Greub, G. & Raoult, D. ( 2004; ). Microorganisms resistant to free-living amoebae. Clin Microbiol Rev 17, 413–433.[CrossRef]
    [Google Scholar]
  18. Hanne, L. F. & Finkelstein, R. A. ( 1982; ). Characterization and distribution of the hemagglutinins produced by Vibrio cholerae. Infect Immun 36, 209–214.
    [Google Scholar]
  19. Jeong, H. J., Jang, E. S., Han, B. I., Lee, K. H., Ock, M. S., Kong, H. H., Chung, D. I., Seol, S. Y., Cho, D. T. & Yu, H. S. ( 2007; ). Acanthamoeba: could it be an environmental host of Shigella? Exp Parasitol 115, 181–186.[CrossRef]
    [Google Scholar]
  20. Johnson, J. A., Salles, C. A., Panigrahi, P., Albert, M. J., Wright, A. C., Johnson, R. J. & Morris, J. G., Jr ( 1994; ). Vibrio cholerae O139 synonym Bengal is closely related to Vibrio cholerae El Tor but has important differences. Infect Immun 62, 2108–2110.
    [Google Scholar]
  21. King, C. H., Shotts, E. B., Jr, Wooley, R. E. & Porter, K. G. ( 1988; ). Survival of coliforms and bacterial pathogens within protozoa during chlorination. Appl Environ Microbiol 54, 3023–3033.
    [Google Scholar]
  22. Knirel, Y. A., Widmalm, G., Senchenkova, S. N., Jansson, P. E. & Weintraub, A. ( 1997; ). Structural studies on the short-chain lipopolysaccharide of Vibrio cholerae O139 Bengal. Eur J Biochem 247, 402–410.[CrossRef]
    [Google Scholar]
  23. La Scola, B. & Raoult, D. ( 2001; ). Survival of Coxiella burnetii within free-living amoeba Acanthamoeba castellanii. Clin Microbiol Infect 7, 75–79.
    [Google Scholar]
  24. Lock, R., Öhman, L. & Dahlgren, C. ( 1987; ). Phagocytic recognition mechanisms in human granulocytes and Acanthamoeba castellanii using type 1 fimbriated Escherichia coli as phagocytic prey. FEMS Microbiol Lett 44, 135–140.[CrossRef]
    [Google Scholar]
  25. Lu, L. & Walker, W. A. ( 2001; ). Pathologic and physiologic interactions of bacteria with the gastrointestinal epithelium. Am J Clin Nutr 73, 1124S–1130S.
    [Google Scholar]
  26. Ly, T. M. & Muller, H. E. ( 1990; ). Ingested Listeria monocytogenes survive and multiply in protozoa. J Med Microbiol 33, 51–54.[CrossRef]
    [Google Scholar]
  27. Nesper, J., Schild, S., Lauriano, C. M., Kraiss, A., Klose, K. E. & Reidl, J. ( 2002; ). Role of Vibrio cholerae O139 surface polysaccharides in intestinal colonization. Infect Immun 70, 5990–5996.[CrossRef]
    [Google Scholar]
  28. Oates, P. J. & Touster, O. ( 1976; ). In vitro fusion of Acanthamoebaphagolysosomes. I. Demonstration and quantitation of vacuole fusion in Acanthamoebahomogenates. J Cell Biol 68 , 319 –338. [CrossRef]
    [Google Scholar]
  29. Pettit, D. A., Williamson, J., Cabral, G. A. & Marciano-Cabral, F. ( 1996; ). In vitro destruction of nerve cell cultures by Acanthamoeba spp.: a transmission and scanning electron microscopy study. J Parasitol 82, 769–777.[CrossRef]
    [Google Scholar]
  30. Saeed, A., Abd, H., Edvinsson, B. & Sandstrom, G. ( 2007; ). Vibrio cholerae–Acanthamoeba castellanii interaction showing endosymbiont–host relation. Symbiosis 44, 153–158.
    [Google Scholar]
  31. Snelling, W. J., Moore, J. E., McKenna, J. P., Lecky, D. M. & Dooley, J. S. ( 2006; ). Bacterial-protozoa interactions; an update on the role these phenomena play towards human illness. Microbes Infect 8, 578–587.[CrossRef]
    [Google Scholar]
  32. Steinert, M., Birkness, K., White, E., Fields, B. & Quinn, F. ( 1998; ). Mycobacterium avium bacilli grow saprozoically in coculture with Acanthamoeba polyphaga and survive within cyst walls. Appl Environ Microbiol 64, 2256–2261.
    [Google Scholar]
  33. Tarsi, R. & Pruzzo, C. ( 1999; ). Role of surface proteins in Vibrio cholerae attachment to chitin. Appl Environ Microbiol 65, 1348–1351.
    [Google Scholar]
  34. Waldor, M. K., Colwell, R. & Mekalanos, J. J. ( 1994; ). The Vibrio cholerae O139 serogroup antigen includes an O-antigen capsule and lipopolysaccharide virulence determinants. Proc Natl Acad Sci U S A 91, 11388–11392.[CrossRef]
    [Google Scholar]
  35. Weekers, P. H., Bodelier, P. L., Wijen, J. P. & Vogels, G. D. ( 1993; ). Effects of grazing by the free-living soil amoebae Acanthamoeba castellanii, Acanthamoeba polyphaga, and Hartmannella vermiformis on various bacteria. Appl Environ Microbiol 59, 2317–2319.
    [Google Scholar]
  36. Weintraub, A., Widmalm, G., Jansson, P. E., Jansson, M., Hultenby, K. & Albert, M. J. ( 1994; ). Vibrio cholerae O139 Bengal possesses a capsular polysaccharide which may confer increased virulence. Microb Pathog 16, 235–241.[CrossRef]
    [Google Scholar]
  37. Winiecka-Krusnell, J., Wreiber, K., von Euler, A., Engstrand, L. & Linder, E. ( 2002; ). Free-living amoebae promote growth and survival of Helicobacter pylori. Scand J Infect Dis 34, 253–256.[CrossRef]
    [Google Scholar]
  38. Yamamoto, T., Albert, M. J. & Sack, R. B. ( 1994; ). Adherence to human small intestines of capsulated Vibrio cholerae O139. FEMS Microbiol Lett 119, 229–235.[CrossRef]
    [Google Scholar]
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