1887

Abstract

The prebiotic Bimuno is a mixture containing galactooligosaccharide, produced by the galactosyltransferase activity of NCIMB 41171 in the presence of lactose. Previous studies have implicated prebiotics in reducing infections by enteric pathogens, thus it was hypothesized that Bimuno may confer some protection in the murine host from serovar Typhimurium ( Typhimurium) infection. In this study, infection caused by Typhimurium SL1344nal in the presence or absence of Bimuno was assessed using tissue culture assays, a murine ligated ileal gut loop model and a murine oral challenge model. In tissue culture adherence and invasion assays with HT-29-16E cells, the presence of ∼2 mM Bimuno significantly reduced the invasion of Typhimurium SL1344nal (<0.0001). In the murine ligated ileal gut loops, the presence of Bimuno prevented colonization and the associated pathology of Typhimurium. In the BALB/c mouse model, the oral delivery of Bimuno prior to challenge with Typhimurium resulted in significant reductions in colonization in the five organs sampled, with highly significant reductions being observed in the spleen at 72 and 96 h post-challenge (=0.0002, <0.0001, respectively). Collectively, the results indicate that Bimuno significantly reduced the colonization and pathology associated with Typhimurium infection in a murine model system, possibly by reducing the invasion of the pathogen into host cells.

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2009-01-01
2019-10-18
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