1887

Abstract

Episodes of cholera stemming from indigenous strains in Australia are mainly associated with environmental sources. In the present study, 10 O1 strains of Australian origin were characterized. All of the strains were serogroup O1 and their conventional phenotypic traits categorized them as belonging to the El Tor biotype. Genetic screening of 12 genomic regions that are associated with virulence in showed variable results. Analysis of the gene showed that the Australian environmental reservoir contains both toxigenic and non-toxigenic strains. DNA sequencing revealed that all of the toxigenic strains examined were of genotype 2. Whole genome PFGE analysis revealed that the environmental toxigenic O1 strains were more diverse than the non-toxigenic environmental O1 strains, and the absence of genes that make up the seventh pandemic island-I and -II in all of the strains indicates their pre-seventh pandemic ancestry.

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2009-01-01
2019-10-15
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