1887

Abstract

Retrospective analysis led to the detection of two variant O1 strains (VC51 and VC53), which were isolated in 1992 in Kolkata from clinical cases, with identical traits to 2004 Mozambique variant O1 strains. The Mozambique O1 strains that caused a huge outbreak in 2004 have been shown to have phenotypic traits of both classical and El Tor biotypes, and thereby have been reported as variant. Our study demonstrated that two O1 strains isolated in Kolkata during 1992 were of the El Tor background as evidenced by polymyxin B (50 U ml) resistance, positivity in Voges–Proskauer reactions and sensitivity to biotype-specific vibrio phages. With the features of classical CTX prophage, localization in the small chromosome, and an absence of RS1 and pTLC, both Mozambique and Kolkata strains appeared to be identical. Furthermore, two Kolkata strains exhibited an identical ribotype to that of the Mozambique variant, displaying ribotype pattern RI that had been assigned to Kolkata O1 strains isolated on or before 1992. I pulsotype analysis indicated that these 1992 Kolkata strains along with the Mozambique variant O1 belonged to very closely related clones. Considering the chronological events, and the typical identity at the phenotypic and the genotypic level between the two O1 strains isolated during 1992 from Kolkata and during 2004 from Mozambique, we propose that some of the 1992 Kolkata O1 strains might have acted as progenitors for Mozambique variant O1 strains.

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2009-02-01
2019-10-21
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References

  1. Ansaruzzaman, M., Bhuiyan, N. A., Nair, B. G., Sack, D. A., Lucas, M., Deen, J. L., Ampuero, J. & Chaignat, C. L. ( 2004; ). Cholera in Mozambique, variant of Vibrio cholerae. Emerg Infect Dis 10, 2057–2059.[CrossRef]
    [Google Scholar]
  2. Ansaruzzaman, M., Bhuiyan, N. A., Safa, A., Sultana, M., McUamule, A., Mondlane, C., Wang, X. Y., Deen, J. L., von Seidlein, L. & other authors ( 2007; ). Genetic diversity of El Tor strains of Vibrio cholerae O1 with hybrid traits isolated from Bangladesh and Mozambique. Int J Med Microbiol 297, 443–449.[CrossRef]
    [Google Scholar]
  3. Barrett, T. J. & Blake, P. A. ( 1981; ). Epidemiological usefulness of changes in hemolytic activity of Vibrio cholerae biotype El Tor during the seventh pandemic. J Clin Microbiol 13, 126–129.
    [Google Scholar]
  4. Bhattacharya, T., Chatterjee, S., Maiti, D., Bhadra, R. K., Takeda, Y., Nair, G. B. & Nandy, R. K. ( 2006; ). Molecular analysis of the rstR and orfU genes of the CTX prophages integrated in the small chromosomes of environmental Vibrio cholerae non-O1, non-O139 strains. Environ Microbiol 8, 526–534.[CrossRef]
    [Google Scholar]
  5. Bik, E. M., Gouw, R. D. & Mooi, F. R. ( 1996; ). DNA fingerprinting of Vibrio cholerae strains with a novel insertion sequence element: a tool to identify epidemic strains. J Clin Microbiol 34, 1453–1461.
    [Google Scholar]
  6. Brosius, J., Ullrich, A., Raker, M. A., Gray, A., Dull, T. J., Gutell, R. R. & Noller, H. F. ( 1981; ). Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli. Plasmid 6, 112–118.[CrossRef]
    [Google Scholar]
  7. Chakrabarti, A. K., Ghosh, A. N., Nair, G. B., Niyogi, S. K., Bhattacharya, S. K. & Sarkar, B. L. ( 2000; ). Development and evaluation of a phage typing scheme for Vibrio cholerae O139. J Clin Microbiol 38, 44–49.
    [Google Scholar]
  8. Chatterjee, S., Ghosh, K., Raychoudhuri, A., Pan, A., Bhattacharya, M. K., Mukhopadhyay, A. K., Ramamurthy, T., Bhattacharya, S. K. & Nandy, R. K. ( 2007; ). Phenotypic and genotypic traits and epidemiological implication of Vibrio cholerae O1 and O139 strains in India during 2003. J Med Microbiol 56, 824–832.[CrossRef]
    [Google Scholar]
  9. Das, B., Halder, K., Pal, P. & Bhadra, R. K. ( 2007; ). Small chromosomal integration site of classical CTX prophage in Mozambique Vibrio cholerae O1 biotype El Tor strain. Arch Microbiol 188, 677–683.[CrossRef]
    [Google Scholar]
  10. Davis, B. M., Kimsey, H. H., Chang, W. & Waldor, M. K. ( 1999; ). The Vibrio cholerae O139 Calcutta bacteriophage CTXφ is infectious and encodes a novel repressor. J Bacteriol 181, 6779–6787.
    [Google Scholar]
  11. Davis, B. M., Moyer, K. E., Boyd, E. F. & Waldor, M. K. ( 2000; ). CTX prophages in classical biotype Vibrio cholerae: functional phage genes but dysfunctional phage genomes. J Bacteriol 182, 6992–6998.[CrossRef]
    [Google Scholar]
  12. Faruque, S. M., Tam, V. C., Chowdhury, N., Diraphat, P., Dziejman, M., Heidelberg, J. F., Clemens, J. D., Mekalanos, J. J. & Nair, G. B. ( 2007; ). Genomic analysis of the Mozambique strain of Vibrio cholerae O1 reveals the origin of El Tor strains carrying classical CTX prophage. Proc Natl Acad Sci U S A 104, 5151–5156.[CrossRef]
    [Google Scholar]
  13. Kaper, J. B., Morris, J. G., Jr & Levine, M. M. ( 1995; ). Cholera. Clin Microbiol Rev 8, 48–86.
    [Google Scholar]
  14. Keasler, S. P. & Hall, R. H. ( 1993; ). Detecting and biotyping Vibrio cholerae O1 with multiplex polymerase chain reaction. Lancet 341, 1661 [CrossRef]
    [Google Scholar]
  15. Kelly, M. T., Hickman-Brenner, F. W. & Farmer, J. J., III ( 1992; ). Vibrio. In Manual of Clinical Microbiology, pp. 384–395. Edited by A. Balows, W. J. Hausler, Jr, K. L. Herrmann, H. D. Isenberg & H. J. Shadomy. Washington, DC: American Society for Microbiology.
  16. Kimsey, H. H. & Waldor, M. K. ( 1998; ). CTXφ immunity: application in the development of cholera vaccines. Proc Natl Acad Sci U S A 95, 7035–7039.[CrossRef]
    [Google Scholar]
  17. Maiti, D., Das, B., Saha, A., Nandy, R. K., Nair, G. B. & Bhadra, R. K. ( 2006; ). Genetic organization of pre-CTX and CTX prophages in the genome of an environmental Vibrio cholerae non-O1, non-O139 strain. Microbiology 152, 3633–3641.[CrossRef]
    [Google Scholar]
  18. Murray, M. G. & Thompson, W. F. ( 1980; ). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8, 4321–4325.[CrossRef]
    [Google Scholar]
  19. Nair, G. B., Faruque, S. M., Bhuiyan, N. A., Kamruzzaman, M., Siddique, A. K. & Sack, D. A. ( 2002; ). New variants of Vibrio cholerae O1 biotype El Tor with attributes of the classical biotype from hospitalized patients with acute diarrhea in Bangladesh. J Clin Microbiol 40, 3296–3299.[CrossRef]
    [Google Scholar]
  20. Olsvik, O., Wahlberg, J., Petterson, B., Uhlen, M., Popovic, T., Wachsmuth, I. K. & Fields, P. I. ( 1993; ). Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains. J Clin Microbiol 31, 22–25.
    [Google Scholar]
  21. Popovic, T., Fields, P. I. & Olsvik, O. ( 1994; ). Detection of cholera toxin genes. In Vibrio cholerae and Cholera: Molecular to Global Perspectives, pp. 41–52. Edited by I. K. Wachsmuth, P. A. Blake & O. Olsvik. Washington, DC: American Society for Microbiology.
  22. Rubin, E. J., Lin, W., Mekalanos, J. J. & Waldor, M. K. ( 1998; ). Replication and integration of a Vibrio cholerae cryptic plasmid linked to the CTX prophage. Mol Microbiol 28, 1247–1254.[CrossRef]
    [Google Scholar]
  23. Safa, A., Bhuyian, N. A., Nusrin, S., Ansaruzzaman, M., Alam, M., Hamabata, T., Takeda, Y., Sack, D. A. & Nair, G. B. ( 2006; ). Genetic characteristics of Matlab variants of Vibrio cholerae O1 that are hybrids between classical and El Tor biotypes. J Med Microbiol 55, 1563–1569.[CrossRef]
    [Google Scholar]
  24. Sharma, C., Nair, G. B., Mukhopadhyay, A. K., Bhattacharya, S. K., Ghosh, R. K. & Ghosh, A. ( 1997; ). Molecular characterization of Vibrio cholerae O1 biotype El Tor strains isolated between 1992 and 1995 in Calcutta, India: evidence for the emergence of a new clone of the El Tor biotype. J Infect Dis 175, 1134–1141.[CrossRef]
    [Google Scholar]
  25. Trucksis, M., Michalski, J., Deng, Y. K. & Kaper, J. B. ( 1998; ). The Vibrio cholerae genome contains two unique circular chromosomes. Proc Natl Acad Sci U S A 95, 14464–14469.[CrossRef]
    [Google Scholar]
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