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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 74, Issue 5

Alterations of ocular surface microbiome in glaucoma and its association with dry eye Open Access

Abstract

Alterations in ocular surface microbiota (OSM) have been noted in both dry eye disease (DED) and glaucoma. However, the combined effects of these conditions on OSM have not been explored.

We hypothesized that patients with both glaucoma and dry eye would exhibit distinct changes in OSM composition and diversity compared to those with only glaucoma, only dry eye or healthy individuals.

We employed amplicon sequencing to investigate OSM profiles in patients with glaucoma and/or dry eye disease.

Swab samples from the conjunctiva of both eyes were collected from 28 glaucomatous patients [13 without dry eye syndrome (G-only) and 15 with dry eye syndrome (G-DED)], 13 DED patients without glaucoma (DED-only) and 31 age-matched healthy controls (HCs). After V3–V4 16S rRNA sequencing, MOTHUR tools and R language were used to elucidate and compare OSM composition and diversity between groups.

Our data revealed very diverse bacterial communities with 28 phyla and 785 genera. All the groups shared the three most abundant phyla, (67.47%), (17.14%) and (13.73%). (54.75%), (10.71%), (8.77%) and (3.20%) were the most abundant genera. Only the G-DED group showed higher alpha diversity than the HC group (<0.05). However, significant differences in beta diversity were observed between all three patient groups and the HC group. The Differential Expression for Sequencing 2 (DESeq2) analysis unveiled an increased presence of opportunistic bacteria across all pathological groups, with the G-DED group demonstrating the most pronounced alterations.

Our findings confirm the predominance of Gram-positive bacteria in normal OSM and the rise of opportunistic Gram-negative bacteria in glaucoma and dry eye disease. This is the first study to characterize OSM in glaucoma patients with DED.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): conjunctiva , dry eye disease , dysbiosis , glaucoma , microbiome and preservatives
© 2025 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. The Microbiology Society waived the open access fees for this article.
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2025-12-15

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References

  1. Tham YC, Li X, Wong TY, Quigley HA, Aung T et al. Global prevalence of glaucoma and projections of glaucoma burden through 2040: a systematic review and meta-analysis. Ophthalmology 2014; 121:2081–2090
     [Google Scholar]
  2. Yıldırım N, Bozkurt B, Yüksel N, Ateş H, Altan-Yaycıoğlu R et al. Prevalence of ocular surface disease and associated risk factors in glaucoma patients: a survey study of ophthalmologists. Turk J Ophthalmol 2022; 52:302–308 [View Article] [PubMed]
     [Google Scholar]
  3. Spörri L, Uldry A-C, Kreuzer M, Herzog EL, Zinkernagel MS et al. Exploring the ocular surface microbiome and tear proteome in glaucoma. Int J Mol Sci 2024; 25:6257 [View Article] [PubMed]
     [Google Scholar]
  4. Huang L, Hong Y, Fu X, Tan H, Chen Y et al. The role of the microbiota in glaucoma. Mol Aspects Med 2023; 94:101221 [View Article] [PubMed]
     [Google Scholar]
  5. Chang C-CJ, Somohano K, Zemsky C, Uhlemann A-C, Liebmann J et al. Topical glaucoma therapy Is associated with alterations of the ocular surface microbiome. Invest Ophthalmol Vis Sci 2022; 63:32 [View Article] [PubMed]
     [Google Scholar]
  6. Honda R, Toshida H, Suto C, Fujimaki T, Kimura T et al. Effect of long-term treatment with eyedrops for glaucoma on conjunctival bacterial flora. Infect Drug Resist 2011; 4:191–196 [View Article] [PubMed]
     [Google Scholar]
  7. Monjane MJ, Makupa W. Prevalence and associated factors of dry eye among glaucoma patients at KCMC eye department. OJOph 2020; 10:154–163 [View Article]
     [Google Scholar]
  8. Leila K, Gatfaoui F, Mahjoub A, Yakoubi S, Krifa F et al. Impact du traitement antiglaucomateux et de la pathologie de la surface oculaire sur la qualité de vie des patients glaucomateux dans la région de Sousse (Tunisie). J Fr Ophtalmol 2019; 42:464–470 [View Article]
     [Google Scholar]
  9. Nijm LM, De Benito-Llopis L, Rossi GC, Vajaranant TS, Coroneo MT. Understanding the dual dilemma of dry eye and glaucoma: an international review. Asia Pac J Ophthalmol 2020; 9:481–490 [View Article] [PubMed]
     [Google Scholar]
  10. Costa VP, Marcon IM, Galvão Filho RP, Malta RFS. The prevalence of ocular surface complaints in Brazilian patients with glaucoma or ocular hypertension. Arq Bras Oftalmol 2013; 76:221–225 [View Article]
     [Google Scholar]
  11. Erb C, Gast U, Schremmer D. German register for glaucoma patients with dry eye. I. Basic outcome with respect to dry eye. Graefes Arch Clin Exp Ophthalmol 2008; 246:1593–1601 [View Article] [PubMed]
     [Google Scholar]
  12. Rossi GCM, Pasinetti GM, Scudeller L, Bianchi PE. Ocular surface disease and glaucoma: how to evaluate impact on quality of life. J Ocul Pharmacol Ther 2013; 29:390–394 [View Article] [PubMed]
     [Google Scholar]
  13. Ruangvaravate N, Prabhasawat P, Vachirasakchai V, Tantimala R. High prevalence of ocular surface disease among glaucoma patients in Thailand. J Ocul Pharmacol Ther 2018; 34:387–394 [View Article] [PubMed]
     [Google Scholar]
  14. Stalmans I, Lemij H, Clarke J, Baudouin C. GOAL study group Signs and symptoms of ocular surface disease: the reasons for patient dissatisfaction with glaucoma treatments. Clin Ophthalmol 2020; 14:3675–3680 [View Article] [PubMed]
     [Google Scholar]
  15. Nijm LM, Schweitzer J, Gould Blackmore J. Glaucoma and dry eye disease: opportunity to assess and treat. Clin Ophthalmol 2023; 17:3063–3076 [View Article] [PubMed]
     [Google Scholar]
  16. Baudouin C, Renard J-P, Nordmann J-P, Denis P, Lachkar Y et al. Prevalence and risk factors for ocular surface disease among patients treated over the long term for glaucoma or ocular hypertension. Eur J Ophthalmol 2012 [View Article]
     [Google Scholar]
  17. Benitez-Del-Castillo J, Cantu-Dibildox J, Sanz-González SM, Zanón-Moreno V, Pinazo-Duran MD. Cytokine expression in tears of patients with glaucoma or dry eye disease: a prospective, observational cohort study. Eur J Ophthalmol 2019; 29:437–443 [View Article] [PubMed]
     [Google Scholar]
  18. Chen HY, Lin CL, Tsai YY, Kao CH. Association between glaucoma medication usage and dry eye in Taiwan. Optom Vis Sci 2015; 92:e227–32 [View Article] [PubMed]
     [Google Scholar]
  19. Kobia-Acquah E, Gyekye GA, Antwi-Adjei EK, Koomson NY, Cobbina F et al. Assessment of ocular surface disease in glaucoma patients in Ghana. J Glaucoma 2021; 30:180–186 [View Article] [PubMed]
     [Google Scholar]
  20. Kim DW, Seo JH, Lim SH. Evaluation of ocular surface disease in elderly patients with glaucoma: expression of matrix metalloproteinase-9 in tears. Eye 2021; 35:892–900 [View Article]
     [Google Scholar]
  21. Mohammed I, Kulkarni B, Faraj LA, Abbas A, Dua HS et al. Profiling ocular surface responses to preserved and non‐preserved topical glaucoma medications: A 2‐year randomized evaluation study. Clin Exp Ophthalmol 2020; 48:973–982 [View Article]
     [Google Scholar]
  22. Tirpack AR, Vanner E, Parrish JM, Galor A, Hua H-U et al. Dry eye symptoms and ocular pain in veterans with glaucoma. J Clin Med 2019; 8:1076 [View Article] [PubMed]
     [Google Scholar]
  23. Wong TT, Zhou L, Li J, Tong L, Zhao SZ et al. Proteomic profiling of inflammatory signaling molecules in the tears of patients on chronic glaucoma medication. Invest Ophthalmol Vis Sci 2011; 52:7385–7391 [View Article] [PubMed]
     [Google Scholar]
  24. Larkin DF, Leeming JP. Quantitative alterations of the commensal eye bacteria in contact lens wear. Eye 1991; 5 (pt 1):70–74 [View Article]
     [Google Scholar]
  25. Chiang MC, Chern E. Ocular surface microbiota: ophthalmic infectious disease and probiotics. Front Microbiol 2022; 13:952473 [View Article] [PubMed]
     [Google Scholar]
  26. Hou K, Wu Z-X, Chen X-Y, Wang J-Q, Zhang D et al. Microbiota in health and diseases. Signal Transduct Target Ther 2022; 7:1–28 [View Article] [PubMed]
     [Google Scholar]
  27. Dong X, Wang Y, Wang W, Lin P, Huang Y. Composition and diversity of bacterial community on the ocular surface of patients with meibomian gland Dysfunction. Invest Ophthalmol Vis Sci 2019; 60:4774–4783 [View Article] [PubMed]
     [Google Scholar]
  28. Shin JH, Lee JW, Lim SH, Yoon BW, Lee Y et al. The microbiomes of the eyelid and buccal area of patients with uveitic glaucoma. BMC Ophthalmol 2022; 22:170 [View Article] [PubMed]
     [Google Scholar]
  29. Ohtani S, Shimizu K, Nejima R, Kagaya F, Aihara M. Conjunctival bacteria flora of glaucoma patients during long-term administration of prostaglandin analog drops. Invest Ophthalmol Vis Sci 2017; 58:3991 [View Article]
     [Google Scholar]
  30. Lim SH, Shin JH, Lee JW, Lee Y, Seo JH. Differences in the eyelid and buccal microbiome of glaucoma patients receiving long-term administration of prostaglandin analog drops. Graefes Arch Clin Exp Ophthalmol 2021; 259:3055–3065 [View Article] [PubMed]
     [Google Scholar]
  31. Kittipibul T, Puangsricharern V, Chatsuwan T. Comparison of the ocular microbiome between chronic Stevens-Johnson syndrome patients and healthy subjects. Sci Rep 2020; 10:4353 [View Article] [PubMed]
     [Google Scholar]
  32. Li Z, Gong Y, Chen S, Li S, Zhang Y et al. Comparative portrayal of ocular surface microbe with and without dry eye. J Microbiol 2019; 57:1025–1032 [View Article]
     [Google Scholar]
  33. Shimizu E, Ogawa Y, Saijo Y, Yamane M, Uchino M et al. Commensal microflora in human conjunctiva; characteristics of microflora in the patients with chronic ocular graft-versus-host disease. Ocul Surf 2019; 17:265–271 [View Article] [PubMed]
     [Google Scholar]
  34. Willis KA, Postnikoff CK, Freeman A, Rezonzew G, Nichols K et al. The closed eye harbours a unique microbiome in dry eye disease. Sci Rep 2020; 10:12035 [View Article] [PubMed]
     [Google Scholar]
  35. Foulks GN. The correlation between the tear film lipid layer and dry eye disease. Surv Ophthalmol 2007; 52:369–374 [View Article] [PubMed]
     [Google Scholar]
  36. Gupta N, Chhibber-Goel J, Gupta Y, Mukherjee S, Maitra A et al. Ocular conjunctival microbiome profiling in dry eye disease: a case control pilot study. Indian J Ophthalmol 2023; 71:1574–1581 [View Article] [PubMed]
     [Google Scholar]
  37. Shih KC, Tong L. The Conjunctival microbiome and dry eye: what we know and controversies. Eye Contact Lens 2024; 50:208–211 [View Article] [PubMed]
     [Google Scholar]
  38. Zhang Z, Zou X, Xue W, Zhang P, Wang S et al. Ocular surface microbiota in diabetic patients with dry eye disease. Invest Ophthalmol Vis Sci 2021; 62:13 [View Article] [PubMed]
     [Google Scholar]
  39. Neyrinck AM, Catry E, Taminiau B, Cani PD, Bindels LB et al. Chitin-glucan and pomegranate polyphenols improve endothelial dysfunction. Sci Rep 2019; 9:14150 [View Article] [PubMed]
     [Google Scholar]
  40. Rognes T, Flouri T, Nichols B, Quince C, Mahé F. VSEARCH: a versatile open source tool for metagenomics. PeerJ 2016; 4:e2584 [View Article] [PubMed]
     [Google Scholar]
  41. Gérard A, El-Hajjaji S, Burteau S, Fall PA, Pirard B et al. Study of the microbial diversity of a panel of Belgian artisanal cheeses associated with challenge studies for Listeria monocytogenes. Food Microbiol 2021; 100:103861 [View Article] [PubMed]
     [Google Scholar]
  42. Jari Oksanen FGB, Kindt R, Legendre P, Minchin PR, O’hara RB et al. Package “vegan”. Community ecology package24 October 2018 2:
     [Google Scholar]
  43. Andersson J, Vogt JK, Dalgaard MD, Pedersen O, Holmgaard K et al. Ocular surface microbiota in patients with aqueous tear-deficient dry eye. Ocul Surf 2021; 19:210–217 [View Article] [PubMed]
     [Google Scholar]
  44. Huang Y, Yang B, Li W. Defining the normal core microbiome of conjunctival microbial communities. Clin Microbiol Infect 2016; 22:643 [View Article] [PubMed]
     [Google Scholar]
  45. Hori Y, Maeda N, Sakamoto M, Koh S, Inoue T et al. Bacteriologic profile of the conjunctiva in the patients with dry eye. Am J Ophthalmol 2008; 146:729–734 [View Article] [PubMed]
     [Google Scholar]
  46. Jiang X, Deng A, Yang J, Bai H, Yang Z et al. Pathogens in the Meibomian gland and conjunctival sac: microbiome of normal subjects and patients with Meibomian gland dysfunction. Infect Drug Resist 2018; 11:1729–1740 [View Article] [PubMed]
     [Google Scholar]
  47. Zhao F, Zhang D, Ge C, Zhang L, Reinach PS et al. Metagenomic profiling of ocular surface microbiome changes in meibomian gland dysfunction. Invest Ophthalmol Vis Sci 2020; 61:22 [View Article] [PubMed]
     [Google Scholar]
  48. Yau JW-K, Hou J, Tsui SKW, Leung TF, Cheng NS et al. Characterization of ocular and nasopharyngeal microbiome in allergic rhinoconjunctivitis. Pediatr Allergy Immunol 2019; 30:624–631 [View Article] [PubMed]
     [Google Scholar]
  49. Lee SH, Oh DH, Jung JY, Kim JC, Jeon CO. Comparative ocular microbial communities in humans with and without blepharitis. Invest Ophthalmol Vis Sci 2012; 53:5585–5593 [View Article] [PubMed]
     [Google Scholar]
  50. Zhou Y, Holland MJ, Makalo P, Joof H, Roberts CH et al. The conjunctival microbiome in health and trachomatous disease: a case control study. Genome Med 2014; 6:99 [View Article] [PubMed]
     [Google Scholar]
  51. Willcox MDP. Characterization of the normal microbiota of the ocular surface. Exp Eye Res 2013; 117:99–105 [View Article] [PubMed]
     [Google Scholar]
  52. Kang Y, Lin S, Ma X, Che Y, Chen Y et al. Strain heterogeneity, cooccurrence network, taxonomic composition and functional profile of the healthy ocular surface microbiome. Eye Vis 2021; 8:6 [View Article] [PubMed]
     [Google Scholar]
  53. Shivaji S, Jayasudha R, Chakravarthy SK, SaiAbhilash CR, Sai Prashanthi G et al. Alterations in the conjunctival surface bacterial microbiome in bacterial keratitis patients. Exp Eye Res 2021; 203:108418 [View Article] [PubMed]
     [Google Scholar]
  54. Yan Y, Yao Q, Lu Y, Shao C, Sun H et al. Association between demodex infestation and ocular surface microbiota in patients with demodex blepharitis. Front Med 2020; 7:592759 [View Article] [PubMed]
     [Google Scholar]
  55. Cavuoto KM, Banerjee S, Miller D, Galor A. Composition and comparison of the ocular surface microbiome in infants and older children. Transl Vis Sci Technol 2018; 7:16 [View Article] [PubMed]
     [Google Scholar]
  56. Chen Z, Xiang Z, Cui L, Qin X, Chen S et al. Significantly different results in the ocular surface microbiome detected by tear paper and conjunctival swab. BMC Microbiol 2023; 23:31 [View Article] [PubMed]
     [Google Scholar]
  57. Dong Q, Brulc JM, Iovieno A, Bates B, Garoutte A et al. Diversity of bacteria at healthy human conjunctiva. Invest Ophthalmol Vis Sci 2011; 52:5408–5413 [View Article] [PubMed]
     [Google Scholar]
  58. Li S, Yi G, Peng H, Li Z, Chen S et al. How ocular surface microbiota debuts in type 2 diabetes mellitus. Front Cell Infect Microbiol 2019; 9:202 [View Article] [PubMed]
     [Google Scholar]
  59. Zou XR, Zhang P, Zhou Y, Yin Y. Ocular surface microbiota in patients with varying degrees of dry eye severity. Int J Ophthalmol 2023; 16:1986–1995 [View Article] [PubMed]
     [Google Scholar]
  60. Fierer N, Hamady M, Lauber CL, Knight R. The influence of sex, handedness, and washing on the diversity of hand surface bacteria. Proc Natl Acad Sci USA 2008; 105:17994–17999 [View Article] [PubMed]
     [Google Scholar]
  61. Doan T, Akileswaran L, Andersen D, Johnson B, Ko N et al. Paucibacterial microbiome and resident DNA virome of the healthy conjunctiva. Invest Ophthalmol Vis Sci 2016; 57:5116–5126 [View Article] [PubMed]
     [Google Scholar]
  62. Ge C, Wei C, Yang BX, Cheng J, Huang YS. Conjunctival microbiome changes associated with fungal keratitis: metagenomic analysis. Int J Ophthalmol 2019; 12:194–200 [View Article] [PubMed]
     [Google Scholar]
  63. Ji X, Dong K, Pu J, Yang J, Zhang Z et al. Comparison of the ocular surface microbiota between thyroid-associated ophthalmopathy patients and healthy subjects. Front Cell Infect Microbiol 2022; 12:914749 [View Article] [PubMed]
     [Google Scholar]
  64. Kim YC, Ham B, Kang KD, Yun JM, Kwon MJ et al. Bacterial distribution on the ocular surface of patients with primary Sjögren’s syndrome. Sci Rep 2022; 12:1715 [View Article] [PubMed]
     [Google Scholar]
  65. Ozkan J, Nielsen S, Diez-Vives C, Coroneo M, Thomas T et al. Temporal stability and composition of the ocular surface microbiome. Sci Rep 2017; 7:9880 [View Article] [PubMed]
     [Google Scholar]
  66. Pickering H, Palmer CD, Houghton J, Makalo P, Joof H et al. Conjunctival microbiome-host responses are associated with impaired epithelial cell health in both early and late stages of trachoma. Front Cell Infect Microbiol 2019; 9:297 [View Article] [PubMed]
     [Google Scholar]
  67. Kugadas A, Christiansen SH, Sankaranarayanan S, Surana NK, Gauguet S et al. Impact of microbiota on resistance to ocular Pseudomonas aeruginosa-induced keratitis. PLoS Pathog 2016; 12:e1005855 [View Article] [PubMed]
     [Google Scholar]
  68. Bolaños-Jiménez R, Navas A, López-Lizárraga EP, de Ribot FM, Peña A et al. Ocular surface as barrier of innate immunity. Open Ophthalmol J 2015; 9:49–55 [View Article] [PubMed]
     [Google Scholar]
  69. St Leger AJ, Desai JV, Drummond RA, Kugadas A, Almaghrabi F et al. An ocular commensal protects against corneal infection by driving an interleukin-17 response from mucosal γδ T cells. Immunity 2017; 47:148–158 [View Article] [PubMed]
     [Google Scholar]
  70. Goldstein MH, Silva FQ, Blender N, Tran T, Vantipalli S. Ocular benzalkonium chloride exposure: problems and solutions. Eye 2022; 36:361–368 [View Article] [PubMed]
     [Google Scholar]
  71. Lee J, Iwasaki T, Ohtani S, Matsui H, Nejima R et al. Benzalkonium chloride resistance in Staphylococcus epidermidis on the ocular surface of glaucoma patients under long-term administration of eye drops. Transl Vis Sci Technol 2020; 9:9 [View Article] [PubMed]
     [Google Scholar]
  72. Fu Y, Wu J, Wang D, Li T, Shi X et al. Metagenomic profiling of ocular surface microbiome changes in Demodex blepharitis patients. Front Cell Infect Microbiol 2022; 12:922753 [View Article] [PubMed]
     [Google Scholar]
  73. Suzuki T, Kinoshita S. Dual role of cutibacterium acnes: commensal bacterium and pathogen in ocular diseases. Microorganisms 2024; 12:1649 [View Article] [PubMed]
     [Google Scholar]
  74. Litvak Y, Byndloss MX, Tsolis RM, Bäumler AJ. Dysbiotic Proteobacteria expansion: a microbial signature of epithelial dysfunction. Curr Opin Microbiol 2017; 39:1–6 [View Article]
     [Google Scholar]
  75. Shin NR, Whon TW, Bae JW. Proteobacteria: microbial signature of dysbiosis in gut microbiota. Trends Biotechnol 2015; 33:496–503 [View Article] [PubMed]
     [Google Scholar]
  76. Sen EM, Yilmaz MB, Dansuk Z, Aksakal FN, Altinok A et al. Effect of chronic topical glaucoma medications on aerobic conjunctival bacterial flora. Cornea 2009; 28:266–270 [View Article] [PubMed]
     [Google Scholar]
  77. Andersson J, Vogt JK, Dalgaard MD, Pedersen O, Holmgaard K et al. Ocular surface microbiota in contact lens users and contact-lens-associated bacterial keratitis. Vision 2021; 5:27 [View Article] [PubMed]
     [Google Scholar]
  78. Graham JE, Moore JE, Jiru X, Moore JE, Goodall EA et al. Ocular pathogen or commensal: a PCR-based study of surface bacterial flora in normal and dry eyes. Invest Ophthalmol Vis Sci 2007; 48:5616–5623 [View Article] [PubMed]
     [Google Scholar]
  79. de Paiva CS, Jones DB, Stern ME, Bian F, Moore QL et al. Altered mucosal microbiome diversity and disease severity in Sjögren syndrome.. Sci Rep 2016; 6:23561 [View Article] [PubMed]
     [Google Scholar]
  80. Liang Q, Li J, Zou Y, Hu X, Deng X et al. Metagenomic analysis reveals the heterogeneity of conjunctival microbiota dysbiosis in dry eye disease.. Front Cell Dev Biol 2021; 9:731867 [View Article] [PubMed]
     [Google Scholar]
  81. Ozkan J, Majzoub ME, Coroneo M, Thomas T, Willcox M. Ocular microbiome changes in dry eye disease and meibomian gland dysfunction. Exp Eye Res 2023; 235:109615 [View Article] [PubMed]
     [Google Scholar]
  82. Watters GA, Turnbull PR, Swift S, Petty A, Craig JP. Ocular surface microbiome in meibomian gland dysfunction. Clin Exp Ophthalmol 2017; 45:105–111 [View Article]
     [Google Scholar]
  83. An Q, Zou H. Ocular surface microbiota dysbiosis contributes to the high prevalence of dry eye disease in diabetic patients. Crit Rev Microbiol 2022; 49:1–10 [View Article] [PubMed]
     [Google Scholar]
  84. Teweldemedhin M, Gebreyesus H, Atsbaha AH, Asgedom SW, Saravanan M. Bacterial profile of ocular infections: a systematic review. BMC Ophthalmol 201725 November 2017 17:212 [View Article] [PubMed]
     [Google Scholar]
  85. Ozkan J, Coroneo M, Willcox M, Wemheuer B, Thomas T. Identification and visualization of a Distinct microbiome in ocular surface conjunctival tissue. Invest Ophthalmol Vis Sci 2018; 59:4268–4276 [View Article] [PubMed]
     [Google Scholar]
  86. Simmons KT, Xiao Y, Pflugfelder SC, de Paiva CS. Inflammatory response to lipopolysaccharide on the ocular surface in a murine dry eye model.. Invest Ophthalmol Vis Sci 2016; 57:2443–2451 [View Article] [PubMed]
     [Google Scholar]
  87. Wang C, Schaefer L, Bian F, Yu Z, Pflugfelder SC et al. Dysbiosis modulates ocular surface inflammatory response to lipopolysaccharide.. Invest Ophthalmol Vis Sci 2019; 60:4224–4233 [View Article] [PubMed]
     [Google Scholar]
  88. Sankaridurg PR, Sharma S, Willcox M, Naduvilath TJ, Sweeney DF et al. Bacterial colonization of disposable soft contact lenses is greater during corneal infiltrative events than during asymptomatic extended lens wear.. J Clin Microbiol 2000; 38:4420–4424 [View Article] [PubMed]
     [Google Scholar]
  89. Aragona P, Baudouin C, Benitez Del Castillo JM, Messmer E, Barabino S et al. The ocular microbiome and microbiota and their effects on ocular surface pathophysiology and disorders. Surv Ophthalmol 2021; 66:907–925 [View Article] [PubMed]
     [Google Scholar]
  90. Deng Y, Wen X, Hu X, Zou Y, Zhao C et al. Geographic difference shaped human ocular surface metagenome of young Han Chinese from Beijing, Wenzhou, and Guangzhou cities.. Invest Ophthalmol Vis Sci 2020; 61:47 [View Article] [PubMed]
     [Google Scholar]
  91. Fernández-Rubio ME, Rebolledo-Lara L, Martinez-García M, Alarcón-Tomás M, Cortés-Valdés C. The conjunctival bacterial pattern of diabetics undergoing cataract surgery. Eye (Lond) 2010; 24:825–834 [View Article] [PubMed]
     [Google Scholar]
  92. Liang C, Wang L, Wang X, Jia Y, Xie Q et al. Altered ocular surface microbiota in obesity: a case-control study. Front Cell Infect Microbiol 2024; 14:1356197 [View Article] [PubMed]
     [Google Scholar]
  93. Ozkan J, Willcox M, Wemheuer B, Wilcsek G, Coroneo M et al. Biogeography of the human ocular microbiota. Ocul Surf 2019; 17:111–118 [View Article] [PubMed]
     [Google Scholar]
  94. Suwajanakorn O, Puangsricharern V, Kittipibul T, Chatsuwan T. Ocular surface microbiome in diabetes mellitus. Sci Rep 2022; 12:21527 [View Article] [PubMed]
     [Google Scholar]
  95. Wen X, Miao L, Deng Y, Bible PW, Hu X et al. The influence of age and sex on ocular surface microbiota in healthy adults.. Invest Ophthalmol Vis Sci 2017; 58:6030–6037 [View Article] [PubMed]
     [Google Scholar]
  96. Adam M, Balcı M, Bayhan HA, İnkaya AÇ, Uyar M et al. Conjunctival flora in diabetic and nondiabetic individuals. Turk J Ophthalmol 2015; 45:193–196 [View Article] [PubMed]
     [Google Scholar]
  97. Bilen H, Ates O, Astam N, Uslu H, Akcay G et al. Conjunctival flora in patients with type 1 or type 2 diabetes mellitus. Adv Ther 2007; 24:1028–1035 [View Article] [PubMed]
     [Google Scholar]
  98. Chen Z, Lin S, Xu Y, Lu L, Zou H. Unique composition of ocular surface microbiome in the old patients with dry eye and diabetes mellitus in a community from Shanghai, China. BMC Microbiol 2024; 24:19 [View Article] [PubMed]
     [Google Scholar]
  99. Manaviat MR, Rashidi M, Afkhami-Ardekani M, Shoja MR. Prevalence of dry eye syndrome and diabetic retinopathy in type 2 diabetic patients. BMC Ophthalmol 2008; 8:10 [View Article] [PubMed]
     [Google Scholar]
  100. Martins EN, Alvarenga LS, Höfling-Lima AL, Freitas D, Zorat-Yu MC et al. Aerobic bacterial conjunctival flora in diabetic patients. Cornea 2004; 23:136–142 [View Article] [PubMed]
     [Google Scholar]
  101. Moreno NP, Moreno RD, Sousa LB. Aerobic bacterial microbiota of the conjunctiva in diabetic patients with normal and altered glycated hemoglobin levels in two regions in Brazil. Arq Bras Oftalmol 2014; 77:351–354 [View Article] [PubMed]
     [Google Scholar]
  102. Shao Z, Shan X, Jing L, Wang W, Li W et al. Metagenome investigation of ocular microbiota of cataract patients with and without type 2 diabetes. Transl Vis Sci Technol 2023; 12:1 [View Article] [PubMed]
     [Google Scholar]
  103. Zilliox MJ, Gange WS, Kuffel G, Mores CR, Joyce C et al. Assessing the ocular surface microbiome in severe ocular surface diseases. The Ocular Surface 2020; 18:706–712 [View Article]
     [Google Scholar]
  104. Zhong Y, Fang X, Wang X, Lin Y-A, Wu H et al. Effects of sodium hyaluronate eye drops with or without preservatives on ocular surface bacterial microbiota.. Front Med 2022; 9:793565 [View Article] [PubMed]
     [Google Scholar]
  105. Dong K, Pu J, Yang J, Zhou G, Ji X et al. The species-level microbiota of healthy eyes revealed by the integration of metataxonomics with culturomics and genome analysis. Front Microbiol 20222 September 2022 13:950591 [View Article] [PubMed]
     [Google Scholar]
  106. Xu S, Zhang H. Bacteriological profile of conjunctiva bacterial Flora in Northeast China: a hospital-based study. BMC Ophthalmol 2022; 22:223 [View Article] [PubMed]
     [Google Scholar]
  107. Shin H, Price K, Albert L, Dodick J, Park L et al. Changes in the eye microbiota associated with contact lens wearing.. mBio 2016; 7:e00198 [View Article] [PubMed]
     [Google Scholar]
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Alterations of ocular surface microbiome in glaucoma and its association with dry eye
J. Med. Microbiol. 74, 002013 (2025); https://doi.org/10.1099/jmm.0.002013
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