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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 73, Issue 10

Nicotine promotes pathogenic bacterial growth and biofilm formation in peri-implant No Access

Abstract

Peri-implantitis is a plaque-associated disease that leads to implant loss and arises from bacterial biofilms on the surface of the implant. Smoking is a risk factor for peri-implantitis and impedes treatment effectiveness. Additionally, aryl hydrocarbon receptor (AHR), IL−6, and IL-22 levels are related to peri-implantitis.

We aimed to investigate the effects of nicotine on inflammatory response, bacterial growth and biofilm formation.

We hypothesized that nicotine promoted pathogenic bacterial growth and biofilm formation, thereby aggravating inflammation.

The expression of AHR, IL-6 and IL-22 was measured in peri-implant sulci fluid using quantitative PCR and Western blot analyses. The cementum was incubated with bacterial suspension including , and and treated with 100, 200, 250 and 300 µg ml nicotine, and then, the absorbance and number of colony-forming units were detected. Biofilm formation was evaluated using the tissue culture plate method and safranin O staining. Carbohydrates and proteins were measured by the phenol–sulfuric acid method and the bicinchoninic acid method, respectively.

The results indicated that smoking increased the levels of AHR, IL-6 and IL-22. Functionally, nicotine promoted the growth of , and . Additionally, it promoted the biofilm formation of these bacteria and increased the contents of carbohydrates and proteins.

Nicotine promoted bacterial growth and biofilm build-up, suggesting that smoking may aggravate the progression of peri-implantitis.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bacteria , biofilm formation , nicotine , peri-implantitis and smoking
Funding
This study was supported by the:
  • The Affiliated Hospital of Yunnan University (Award ynkt2019006)
    • Principal Award Recipient: DahaiHuang
© 2024 The Authors
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2024-10-03
2025-11-10

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/content/journal/jmm/10.1099/jmm.0.001897
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Nicotine promotes pathogenic bacterial growth and biofilm formation in peri-implant
J. Med. Microbiol. 73, 001897 (2024); https://doi.org/10.1099/jmm.0.001897
/content/journal/jmm/10.1099/jmm.0.001897
/content/journal/jmm/10.1099/jmm.0.001897
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