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Abstract
Introduction. Rotavirus A is the most common pathogen causing diarrhoea in children less than 5 years, leading to severe complications such as dehydration, electrolyte imbalances, acidosis, myocarditis, convulsions, pneumonia, and other life-threatening conditions.
Gap statement. There is an urgent need for a rapid and efficient nucleic acid detection strategy to enable early diagnosis and treatment, preventing rotavirus transmission and associated complications.
Aim. This article aimed to develop a nuclear acid sequence-based amplification (NASBA)-Cas12a system for detecting rotavirus A using fluorescence intensity or lateral flow strips.
Methodology. The NASBA technology was combined with the clustered regularly interspaced short palindromic repeats-Cas12a system to establish a NASBA-Cas12a system for detecting rotavirus A.
Results. The NASBA-Cas12a system could detect rotavirus A at 37 ℃ within 70 min and had no cross-reactivity with other viruses, achieving a limit of detection of 1.2 copies μl–1. This system demonstrated a sensitivity of 100%, specificity of 90%, positive predictive value of 97.22% and negative predictive value of 100%. The kappa value was 0.933, indicating that the NASBA-Cas12a system was highly consistent with reverse transcription-PCR.
Conclusion. The NASBA-Cas12a system exhibited high sensitivity and specificity for detecting rotavirus A, showing great potential for clinical application.
- Received:
- Accepted:
- Published Online:
Funding
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the Ningbo public welfare project
(Award no. 2022S127)
- Principle Award Recipient: yuechen
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the Innovation Project of Distinguished Medical Team in Ningbo
(Award no. 2022020405)
- Principle Award Recipient: yuechen
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the Ningbo key discipline Pediatrics
(Award no. 2022-B17)
- Principle Award Recipient: yuechen
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Ningbo Clinical Research Center for Children’s Health and Diseases
(Award no. 2019A21002)
- Principle Award Recipient: yuechen
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Ningbo Medical and Health Brand Discipline
(Award PPXK2024-06)
- Principle Award Recipient: JunhuaWu