1887

Abstract

Rotavirus A is the most common pathogen causing diarrhoea in children less than 5 years, leading to severe complications such as dehydration, electrolyte imbalances, acidosis, myocarditis, convulsions, pneumonia, and other life-threatening conditions.

There is an urgent need for a rapid and efficient nucleic acid detection strategy to enable early diagnosis and treatment, preventing rotavirus transmission and associated complications.

This article aimed to develop a nuclear acid sequence-based amplification (NASBA)-Cas12a system for detecting rotavirus A using fluorescence intensity or lateral flow strips.

The NASBA technology was combined with the clustered regularly interspaced short palindromic repeats-Cas12a system to establish a NASBA-Cas12a system for detecting rotavirus A.

The NASBA-Cas12a system could detect rotavirus A at 37 ℃ within 70 min and had no cross-reactivity with other viruses, achieving a limit of detection of 1.2 copies μl. This system demonstrated a sensitivity of 100%, specificity of 90%, positive predictive value of 97.22% and negative predictive value of 100%. The kappa value was 0.933, indicating that the NASBA-Cas12a system was highly consistent with reverse transcription-PCR.

The NASBA-Cas12a system exhibited high sensitivity and specificity for detecting rotavirus A, showing great potential for clinical application.

Funding
This study was supported by the:
  • the Ningbo public welfare project (Award no. 2022S127)
    • Principle Award Recipient: yuechen
  • the Innovation Project of Distinguished Medical Team in Ningbo (Award no. 2022020405)
    • Principle Award Recipient: yuechen
  • the Ningbo key discipline Pediatrics (Award no. 2022-B17)
    • Principle Award Recipient: yuechen
  • Ningbo Clinical Research Center for Children’s Health and Diseases (Award no. 2019A21002)
    • Principle Award Recipient: yuechen
  • Ningbo Medical and Health Brand Discipline (Award PPXK2024-06)
    • Principle Award Recipient: JunhuaWu
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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/content/journal/jmm/10.1099/jmm.0.001892
2024-10-03
2024-11-10
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