1887

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Volume 73, Issue 9

Polymyxin combined with essential oil: one alternative strategy for combating polymyxin-resistant No Access

Abstract

Multidrug-resistant infections present a critical public health due to scarce treatment options and high mortality. L. essential oil (O.geo) is a natural resource rich in eugenol known for its antimicrobial activity.

O.geo may exert effective antimicrobial activity against polymyxin-resistant Klebsiella pneumoniae and, when combined with Polymyxin B (PMB), may exhibit a synergistic effect, enhancing treatment efficacy and reducing antimicrobial resistance.

This study aims to investigate the antimicrobial activity of O.geo against polymyxin-resistant using tests and an model.

The O.geo was obtained by hydrodistillation followed by gas chromatography. The MIC and antibiofilm activity were determined using broth microdilution. Checkerboard and time-kill assays evaluated the combination of O.geo and polymyxin B (PMB), whereas a protein leakage assay verified its action.

Eugenol (39.67%) was a major constituent identified. The MIC of the O.geo alone ranged from 128 to 512 µg ml. The fractional inhibitory concentration index (0.28) and time-kill assay showed a synergism. In addition, O.geo and PMB inhibited biofilm formation and increased protein leakage in the plasma membrane. The treatment was tested using a model, and significantly increased survival without toxicity was observed.

O.geo could be used as a potential therapeutic alternative to combat infections caused by multidrug-resistant bacteria, especially in combination with PMB.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antimicrobial , Caenorhabditis elegans , Gram-negative multidrug-resistant , plants and synergism
Funding
This study was supported by the:
  • Universidade Federal da Grande Dourados
    • Principle Award Recipient: AndressaLeite Ferraz de Melo
  • Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (Award grants 325/2022 process 71/031.898/2022, 113/2023 process 83/024.134/2023 and 76/2023 process 83/013.190/2023)
    • Principle Award Recipient: SimoneSimionatto
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (Award 408778/2022-9 and 307946/2022-3)
    • Principle Award Recipient: SimionattoSimone
© 2024 The Authors
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2024-10-13
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Polymyxin combined with Ocimum gratissimum essential oil: one alternative strategy for combating polymyxin-resistant Klebsiella pneumoniae
J. Med. Microbiol. 73, 001891 (2024); https://doi.org/10.1099/jmm.0.001891
/content/journal/jmm/10.1099/jmm.0.001891
/content/journal/jmm/10.1099/jmm.0.001891
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