1887

Journal of Medical Microbiology logo

Volume 73, Issue 9

Evaluating the accuracy of the MBT Lipid Xtract Kit for assessing colistin resistance in comparison to broth microdilution Open Access

Abstract

Colistin resistance testing methods such as broth microdilution (BMD) are time-consuming and labour intensive for clinical laboratories. MBT Lipid Xtract Kit on MALDI Biotyper Sirius System (Bruker, Billerica, MA, USA) utilizes lipidomic analysis to identify specific cell wall modifications associated with colistin resistance. We compared MBT to BMD (ComASP Colistin, Liofilchem) across 36 Gram-negative isolates (non-resistant MIC ≤2 µg ml, resistant MIC ≥4 µg ml). All samples were tested twice on MBT with discrepant results repeated before assessing categorical agreement between MBT and BMD. 44.4% (16/36) of isolates were colistin resistant via BMD. MBT Lipid Xtract had 80.6% agreement (29/36) with BMD, with 5/7 discrepancies corrected to match upon repeat testing. There was 100% agreement for isolates (=16). The whole-genome sequencing was completed on the two discrepant isolates, with variants within colistin resistance-associated loci identified (MIC 0.5 µg ml: S30T, T246A, N212T, S253G, Q287K and MIC >16 µg ml: S30T, R90insRN, T246A, E57G, S253G). Further evaluation, particularly for non-, of MBT is required prior to implementation in clinical laboratories.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): BMD , colistin , lipidomics , mcr-1 , resistance screening and susceptibility testing
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-09-02
2024-09-14
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/content/journal/jmm/10.1099/jmm.0.001881
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Evaluating the accuracy of the MBT Lipid Xtract Kit for assessing colistin resistance in comparison to broth microdilution
J. Med. Microbiol. 73, 001881 (2024); https://doi.org/10.1099/jmm.0.001881
/content/journal/jmm/10.1099/jmm.0.001881
/content/journal/jmm/10.1099/jmm.0.001881
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