1887

Abstract

Cold plasma is frequently utilized for the purpose of eliminating microbial contaminants. Under optimal conditions, it can function as plasma medicine for treating various diseases, including infections caused by , an opportunistic pathogen that can overgrow in individuals with weakened immune system.

To date, there has been less molecular study on cold plasma-treated .

The study aims to fill the gap in understanding the molecular response of to cold plasma treatment.

This project involved testing a cold plasma generator to determine its antimicrobial effectiveness on ’ planktonic cells. Additionally, the cells’ transcriptomics responses were investigated using RNA sequencing at various treatment durations (1, 3 and 5 min).

The results show that our cold plasma effectively eliminates . Cold plasma treatment resulted in substantial downregulation of important pathways, such as ‘nucleotide metabolism’, ‘DNA replication and repair’, ‘cell growth’, ‘carbohydrate metabolism’ and ‘amino acid metabolism’. This was an indication of cell cycle arrest of to preserve energy consumption under unfavourable conditions. Nevertheless, adapted its GSH antioxidant system to cope with the oxidative stress induced by reactive oxygen species, reactive nitrogen species and other free radicals. The treatment likely led to a decrease in cell pathogenicity as many virulence factors were downregulated.

The study demonstrated the major affected pathways in cold plasma-treated , providing valuable insights into the molecular response of to cold plasma treatment. The findings contribute to the understanding of the antimicrobial efficiency of cold plasma and its potential applications in the field of microbiology.

Funding
This study was supported by the:
  • Jiangsu Province Industry-University-Research Cooperation Project (Award BY2020506)
    • Principle Award Recipient: XinhuaZhang
  • Gusu Innovation and Entrepreneurship Leading Talents Project (Award ZXL2018192)
    • Principle Award Recipient: XinhuaZhang
  • Universiti Teknologi Malaysia (Award 06E00)
    • Principle Award Recipient: ChunShiong Chong
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/content/journal/jmm/10.1099/jmm.0.001841
2024-07-05
2024-07-15
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