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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 73, Issue 2

Identification of consensus head and neck cancer-associated microbiota signatures: a systematic review and meta-analysis of 16S rRNA and The Cancer Microbiome Atlas datasets Open Access

Abstract

Multiple reports have attempted to describe the tumour microbiota in head and neck cancer (HNSC).

However, these have failed to produce a consistent microbiota signature, which may undermine understanding the importance of bacterial-mediated effects in HNSC.

The aim of this study is to consolidate these datasets and identify a consensus microbiota signature in HNSC.

We analysed 12 published HNSC 16S rRNA microbial datasets collected from cancer, cancer-adjacent and non-cancer tissues to generate a consensus microbiota signature. These signatures were then validated using The Cancer Microbiome Atlas (TCMA) database and correlated with the tumour microenvironment phenotypes and patient’s clinical outcome.

We identified a consensus microbial signature at the genus level to differentiate between HNSC sample types, with cancer and cancer-adjacent tissues sharing more similarity than non-cancer tissues. Univariate analysis on 16S rRNA datasets identified significant differences in the abundance of 34 bacterial genera among the tissue types. Paired cancer and cancer-adjacent tissue analyses in 16S rRNA and TCMA datasets identified increased abundance in in cancer tissues and decreased abundance of , and in cancer-adjacent tissues. Furthermore, these bacteria were associated with different tumour microenvironment phenotypes. Notably, high signature was associated with high neutrophil (r=0.37, <0.0001), angiogenesis (r=0.38, <0.0001) and granulocyte signatures (r=0.38, <0.0001) and better overall patient survival [continuous: HR 0.8482, 95 % confidence interval (CI) 0.7758–0.9273, =0.0003].

Our meta-analysis demonstrates a consensus microbiota signature for HNSC, highlighting its potential importance in this disease.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA sequencing , head and neck cancer , meta-analysis and tumour microbiota
Funding
This study was supported by the:
  • University of Adelaide (Award Postgraduate Research Scholarship)
    • Principle Award Recipient: LinhT.H. Mai
  • University of Adelaide (Award Postgraduate Research Scholarship)
    • Principle Award Recipient: FangmeinuoWu
  • University of Adelaide (Award Postgraduate Research Scholarship)
    • Principle Award Recipient: RunhaoLi
  • University of Adelaide (Award Postgraduate Research Scholarship)
    • Principle Award Recipient: KennyYeo
  • Garnett Passe and Rodney Williams Memorial Foundation (Award Senior Fellowship)
    • Principle Award Recipient: SarahVreugde
  • National Health and Medical Research Council (Award APP1196832)
    • Principle Award Recipient: Peter-JohnWormald
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-02-01
2025-04-30
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Identification of consensus head and neck cancer-associated microbiota signatures: a systematic review and meta-analysis of 16S rRNA and The Cancer Microbiome Atlas datasets
J. Med. Microbiol. 73, 001799 (2024); https://doi.org/10.1099/jmm.0.001799
/content/journal/jmm/10.1099/jmm.0.001799
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