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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 72, Issue 9

co-harbouring and isolated from blood samples in Myanmar No Access

Abstract

The spread of coproducing carbapenemases, 16S rRNA methylase and mobile colistin resistance proteins (MCRs) has become a serious public health problem worldwide. This study describes two clinical isolates of coharbouring , and .

Two clinical isolates of resistant to carbapenems and aminoglycosides were obtained from two patients at a hospital in Myanmar. Their minimum inhibitory concentrations (MICs) were determined by broth microdilution methods. The whole-genome sequences were determined by MiSeq and MinION methods. Drug-resistant factors and their genomic environments were determined.

The two isolates showed MICs of ≥4 and ≥1024 µg ml for carbapenems and aminoglycosides, respectively. Two harbouring were susceptible to colistin, with MICs of ≤0.015 µg ml using cation-adjusted Mueller–Hinton broth, but those for colistin were significantly higher (0.5 and 4 µg ml) using brain heart infusion medium. Whole-genome analysis revealed that these isolates coharboured , and . These two isolates showed low MICs of 0.25 µg ml for colistin. Genome analysis revealed that both and were located on IncFIIs plasmids of similar size (81 kb). The was located on IncM2 plasmids of sizes 220 or 313 kb in each isolate. These two isolates did not possess a gene encoding a two-component system, which is thought to regulate the expression of genes.

This is the first report of isolates of coharbouring , and obtained in Myanmar.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA methylase , armA , blaNDM-1 , carbapenem resistance , carbapenemase , colistin resistance , Klebsiella pneumoniae , mcr-10 and phosphoethanolamine transferase
Funding
This study was supported by the:
  • Japan Society for Aeronautical and Space Sciences (Award 23K06550)
    • Principle Award Recipient: TeruoKirikae
  • Japan Society for the Promotion of Science (Award 21K07031)
    • Principle Award Recipient: TatsuyaTada
  • AMED (Award 22jm0110026h0001)
    • Principle Award Recipient: TeruoKirikae
  • JICA Research Institute (Award SATREPS)
    • Principle Award Recipient: TeruoKirikae
© 2023 The Authors
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/content/journal/jmm/10.1099/jmm.0.001750
2023-09-14
2025-01-16
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/content/journal/jmm/10.1099/jmm.0.001750
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Klebsiella pneumoniae co-harbouring blaNDM-1, armA and mcr-10 isolated from blood samples in Myanmar
J. Med. Microbiol. 72, 001750 (2023); https://doi.org/10.1099/jmm.0.001750
/content/journal/jmm/10.1099/jmm.0.001750
/content/journal/jmm/10.1099/jmm.0.001750
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