1887

Abstract

was once recognised as a benign plant-endosymbiont but recent case reports suggest that it is a newly emerging Gram-negative pathogen related to opportunistic infection of multiple sites in humans.

Antimicrobial susceptibility testing was performed using broth microdilution method. To identify colistin resistance mechanisms, , , and were sequenced and their mRNA expression was analysed using quantitative real-time PCR. In addition, we tried to detect and . The lipid A moieties of colistin-susceptible and -resistant isolates were analysed using MALDI-TOF.

Among the two isolates, one is colistin-resistant, and another is colistin-susceptible. The colistin-resistant isolate showed no mutations in , and , and and were not identified. However, its and expression was significantly higher and amino-arabinosylated lipid A with hexa-acylated species in lipopolysaccharide was identified.

We found that colistin resistance in was mediated by the modification of lipid A. Although the isolate was obtained from faecal samples of healthy adults, colistin-resistant challenges public health as an opportunistic pathogen.

Funding
This study was supported by the:
  • National Research Foundation of Korea (Award 2022R1A2B5B02001716)
    • Principle Award Recipient: KwanSoo Ko
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/content/journal/jmm/10.1099/jmm.0.001680
2023-06-01
2024-04-25
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