1887

Abstract

was once recognised as a benign plant-endosymbiont but recent case reports suggest that it is a newly emerging Gram-negative pathogen related to opportunistic infection of multiple sites in humans.

Antimicrobial susceptibility testing was performed using broth microdilution method. To identify colistin resistance mechanisms, , , and were sequenced and their mRNA expression was analysed using quantitative real-time PCR. In addition, we tried to detect and . The lipid A moieties of colistin-susceptible and -resistant isolates were analysed using MALDI-TOF.

Among the two isolates, one is colistin-resistant, and another is colistin-susceptible. The colistin-resistant isolate showed no mutations in , and , and and were not identified. However, its and expression was significantly higher and amino-arabinosylated lipid A with hexa-acylated species in lipopolysaccharide was identified.

We found that colistin resistance in was mediated by the modification of lipid A. Although the isolate was obtained from faecal samples of healthy adults, colistin-resistant challenges public health as an opportunistic pathogen.

Funding
This study was supported by the:
  • National Research Foundation of Korea (Award 2022R1A2B5B02001716)
    • Principle Award Recipient: KwanSoo Ko
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/content/journal/jmm/10.1099/jmm.0.001680
2023-06-01
2024-07-19
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References

  1. Gipson KS, Nickerson KP, Drenkard E, Llanos-Chea A, Dogiparthi SK et al. The great ESKAPE: exploring the crossroads of bile and antibiotic resistance in bacterial pathogens. Infect Immun 2020; 88:e00865-19 [View Article] [PubMed]
    [Google Scholar]
  2. Rodríguez-Medina N, Barrios-Camacho H, Duran-Bedolla J, Garza-Ramos U. Klebsiella variicola: an emerging pathogen in humans. Emerg Microbes Infect 2019; 8:973–988 [View Article] [PubMed]
    [Google Scholar]
  3. Joo EJ, Kim SJ, Baek M, Choi Y, Seo J et al. Fecal carriage of antimicrobial-resistant enterobacteriaceae in healthy Korean adults. J Microbiol Biotechnol 2018; 28:1178–1184 [View Article] [PubMed]
    [Google Scholar]
  4. Clinical and Laboratory Standards Institute (CLSI) Performance Standards for Antimicrobial Susceptibility Testing, 28th ed. Wayne, PA, USA: CLSI supplement M100; 2018
    [Google Scholar]
  5. Wright MS, Suzuki Y, Jones MB, Marshall SH, Rudin SD et al. Genomic and transcriptomic analyses of colistin-resistant clinical isolates of Klebsiella pneumoniae reveal multiple pathways of resistance. Antimicrob Agents Chemother 2015; 59:536–543 [View Article] [PubMed]
    [Google Scholar]
  6. Kim SJ, Ko KS. Diverse genetic alterations responsible for post-exposure colistin resistance in populations of the same strain of Klebsiella pneumoniae. Int J Antimicrob Agents 2018; 52:425–429 [View Article] [PubMed]
    [Google Scholar]
  7. Yi EC, Hackett M. Rapid isolation method for lipopolysaccharide and lipid a from gram-negative bacteria. Analyst 2000; 125:651–656 [View Article] [PubMed]
    [Google Scholar]
  8. López-Fernández H, Santos HM, Capelo JL, Fdez-Riverola F, Glez-Peña D et al. Mass-Up: an all-in-one open software application for MALDI-TOF mass spectrometry knowledge discovery. BMC Bioinformatics 2015; 16:318 [View Article]
    [Google Scholar]
  9. Leung LM, Cooper VS, Rasko DA, Guo Q, Pacey MP et al. Structural modification of LPS in colistin-resistant, KPC-producing Klebsiella pneumoniae. J Antimicrob Chemother 2017; 72:3035–3042 [View Article] [PubMed]
    [Google Scholar]
  10. Trent MS, Ribeiro AA, Doerrler WT, Lin S, Cotter RJ et al. Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: structural characterization and transfer to lipid A in the periplasm. J Biol Chem 2001; 276:43132–43144 [View Article] [PubMed]
    [Google Scholar]
  11. Vorachek-Warren MK, Ramirez S, Cotter RJ, Raetz CRH. A triple mutant of Escherichia coli lacking secondary acyl chains on lipid A. J Biol Chem 2002; 277:14194–14205 [View Article] [PubMed]
    [Google Scholar]
  12. Lu Y, Feng Y, McNally A, Zong Z. Occurrence of colistin-resistant hypervirulent Klebsiella variicola. J Antimicrob Chemother 2018; 73:3001–3004 [View Article] [PubMed]
    [Google Scholar]
  13. Morales-León F, Opazo-Capurro A, Caro C, Lincopan N, Cardenas-Arias A et al. Hypervirulent and hypermucoviscous extended-spectrum β-lactamase-producing Klebsiella pneumoniae and Klebsiella variicola in Chile. Virulence 2021; 12:35–44 [View Article] [PubMed]
    [Google Scholar]
  14. Janssen AB, Doorduijn DJ, Mills G, Rogers MRC, Bonten MJM et al. Evolution of colistin resistance in the Klebsiella pneumoniae complex follows multiple evolutionary trajectories with variable effects on fitness and virulence characteristics. Antimicrob Agents Chemother 2020; 65:e01958-20 [View Article] [PubMed]
    [Google Scholar]
  15. Skiada A, Markogiannakis A, Plachouras D, Daikos GL. Adaptive resistance to cationic compounds in Pseudomonas aeruginosa. Int J Antimicrob Agents 2011; 37:187–193 [View Article] [PubMed]
    [Google Scholar]
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