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Volume 72, Issue 1

Phenotypic characterization of -specific chicken T-cells responding to parasite antigen re-stimulation Open Access

Abstract

Coccidiosis, caused by protozoan parasites of genus , is a disease with large impact on poultry production worldwide. It is well known that immunity is dependent on Th1-type responses.

assessment of -specific T-cell activity would therefore be a valuable research tool but has so far proven difficult to establish.

The present study aimed to evaluate induced blast transformation and CD25 expression in defined chicken T-cell populations as a measure of immunity.

Three infection experiments were performed and PBMC and/or spleen cells were collected between 6 and 16 days after infection of chickens. Cells were stimulated with antigens and T-cell activation was assessed by immunofluorescence labelling and flow cytometry.

The results consistently showed statistically significant specific activation of TCRα/β+T cells within a ‘window’ from 8 to 14 days after infection for both spleen cells and PBMC. Responding T-cells were identified as CD4+CD8-, CD4+CD8αα+ and CD4-CD8αβ+ where the CD4+CD8αα+ cells generally showed the highest responses. All three of these TCRα/βT-cell subsets showed significant induced blast transformation and/or CD25 expression albeit not always in concert on the same days after infection indicating complex kinetics of T-cell responses. In general, responses were higher for spleen cells compared to PBMC for all responding T-cell populations.

This methodology shows promise to study -specific T-cells, e.g. to evaluate vaccine responses. Results indicated that a Th1-type response was induced and suggested a role for CD4+CD8αα+ cells in immunity.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): blast transformation , CD25 expression , chicken , Eimera tenella and T-cell subpopulations
Funding
This study was supported by the:
  • Svenska Forskningsrådet Formas (Award 2018-00585)
    • Principle Award Recipient: EvaWattrang
  • Svenska Forskningsrådet Formas (Award 942-2015-494)
    • Principle Award Recipient: EvaWattrang
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-01-18
2024-04-16
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Phenotypic characterization of Eimeria tenella-specific chicken T-cells responding to in vitro parasite antigen re-stimulation
J. Med. Microbiol. 72, 001650 (2023); https://doi.org/10.1099/jmm.0.001650
/content/journal/jmm/10.1099/jmm.0.001650
/content/journal/jmm/10.1099/jmm.0.001650
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