1887

Abstract

. Environmental surveillance for is challenging. There are no internationally agreed recommendations on which method should be used when environmental surveillance is undertaken.

. To compare the detection of by RT-PCR to culture-based methods and to determine which is more sensitive and specific in the clinical environment.

. Forty-four near-patient areas of -positive patients were sampled using contact plates and moistened flocked swabs.

. Detection using moistened flocked swabs followed by RT-PCR or culture detected more than contact plates. The sensitivity and specificity of a RT-PCR assay for compared to the culture methods was 76 and 91 %, respectively.

. Despite the lower sensitivity and specificity, RT-PCR could potentially offer a more rapid and practical alternative.

Funding
This study was supported by the:
  • Astellas Pharma Europe (Award GR 16 1314)
    • Principle Award Recipient: HilaryHumphreys
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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/content/journal/jmm/10.1099/jmm.0.001636
2023-01-09
2024-03-29
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