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Volume 71, Issue 5

Changes in the expression of miR-103a and miR-21: a functional diagnosis of toxocariasis in rats No Access

Abstract

Toxocariasis is a zoonotic parasitic disease caused by migrating nematode worms, species larvae, within tissues. MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression at a post-transcriptional level.

miRNA-based diagnostic biomarkers for toxocariasis are emerging, but there is limited information about the role of many miRNAs and a more detailed diagnostic evaluation of miRNA expression patterns is needed to understand their immunobiological function.

We investigated the expression levels of circulating miRNA 21 and miRNA 103a as potential biomarkers for the prediction and diagnosis of toxocariasis in Wistar rats infected with .

Thirty Wistar rats were inoculated orally with 2500 embryonated eggs via gavage. Serum samples were collected from infected animals and were tested against antigens for 60 days post-infection. The plasma samples were isolated for quantitative real-time PCR (qPCR) assays and qPCR was used to assess transcription levels of miRNA 21 and miRNA 103a.

The prevalence of anti- IgG was detected in 7/30 (23.3 %) infected rats. Molecular analysis of and  showed that expression levels of miRNAs in both groups of -positive and negative samples were the same without significant association. The ratio of housekeeping gene expression () to gene expression of miRNAs 21 and 103a indicated the rate of change (1/1.38 ≈ 0.75 and 1/0.751 ≈ 1.3, respectively).

Our study revealed that and  might play fundamental roles as biomarkers and diagnostic tools for toxocariasis. However, the changes in expression of these miRNAs were not adequate to be used as biomarkers in diagnosis.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ELISA , expression , miRNA 103a , miRNA 21 , quantitative real-time PCR and Toxocara canis
Funding
This study was supported by the:
  • Tehran University of Medical Sciences and Health Services (Award Grant No. 25088)
    • Principle Award Recipient: MohammadBagher Rokni
© 2022 The Authors
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/content/journal/jmm/10.1099/jmm.0.001532
2022-05-26
2024-04-18
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References

  1. Schwartz R, Bidaisee S, Fields PJ, Macpherson MLA, Macpherson CNL. The epidemiology and control of Toxocara canis in puppies. Parasite Epidemiol Control 2022; 16:e00232 [View Article]
     [Google Scholar]
  2. Raissi V, Sohrabi Z, Getso M, Raiesi O, Hashemi Hafshejani S et al. Risk factors and prevalence of toxocariasis in pregnant women and diabetic patients compared to healthy adults in Ilam province, Western Iran. EXCLI J 2018; 17:983–988 [View Article] [PubMed]
     [Google Scholar]
  3. Habluetzel A, Traldi G, Ruggieri S, Attili AR, Scuppa P et al. An estimation of Toxocara canis prevalence in dogs, environmental egg contamination and risk of human infection in the Marche region of Italy. Vet Parasitol 2003; 113:243–252 [View Article] [PubMed]
     [Google Scholar]
  4. Papavasilopoulos V, Pitiriga V, Birbas K, Elefsiniotis J, Bonatsos G et al. Soil contamination by Toxocara canis and human seroprevalence in the Attica region, Greece. Germs 2018; 8:155–161 [View Article] [PubMed]
     [Google Scholar]
  5. Raissi V, Raiesi O, Etemadi S, Firoozeh F, Getso M et al. Environmental soil contamination by Toxocara species eggs in public places of Ilam, Iran. Ann Agric Environ Med 2020; 27:15–18 [View Article] [PubMed]
     [Google Scholar]
  6. Fasihi Karami M, Rafiei A, Raiesi O, Getso M, Akhlaghi E et al. The Relation between Toxocariasis and Toxoplasmosis co-infection and the presence of Rheumatoid Factor (RF) in people with hydatidosis in Southwestern Iran, from 2013 to 2018. J Parasit Dis 2019; 43:379–384 [View Article] [PubMed]
     [Google Scholar]
  7. Raissi V, Masoumi MT, Ibrahim A, Etemadi S, Getso M et al. Spatial analysis of Toxocara spp. eggs in soil as a potential for serious human infection. Comp Immunol Microbiol Infect Dis 2021; 75:101619 [View Article] [PubMed]
     [Google Scholar]
  8. Despommier D. Toxocariasis: clinical aspects, epidemiology, medical ecology, and molecular aspects. Clin Microbiol Rev 2003; 16:265–272 [View Article] [PubMed]
     [Google Scholar]
  9. Zibaei M, Sadjjadi SM, Uga S. Experimental Toxocara cati infection in gerbils and rats. Korean J Parasitol 2010; 48:331–333 [View Article] [PubMed]
     [Google Scholar]
  10. Raissi V, Saber V, zibaei M, Bahadory S, Akhlaghi E et al. Comparison of the prevalence of Toxocara spp. eggs in public parks soils in different seasons, from 2017 to 2018, Tehran Province, Iran. Clin Epidemiol Glob Health 2020; 8:450–454 [View Article]
     [Google Scholar]
  11. Raissi V, Sohrabi N, Bayat F, Etemadi S, Raiesi O et al. Human Toxocariasis in individuals with blood disorders and cancer patients: the first seroepidemiological study in Iran. J Parasit Dis 2021; 45:643–650 [View Article] [PubMed]
     [Google Scholar]
  12. Rostami A, Riahi SM, Holland CV, Taghipour A, Khalili-Fomeshi M et al. Seroprevalence estimates for toxocariasis in people worldwide: A systematic review and meta-analysis. PLoS Negl Trop Dis 2019; 13:e0007809 [View Article] [PubMed]
     [Google Scholar]
  13. Fillaux J, Magnaval J-F. Laboratory diagnosis of human toxocariasis. Vet Parasitol 2013; 193:327–336 [View Article] [PubMed]
     [Google Scholar]
  14. Chen J, Liu Q, Liu G-H, Zheng W-B, Hong S-J et al. Toxocariasis: a silent threat with a progressive public health impact. Infect Dis Poverty 2018; 7:59 [View Article] [PubMed]
     [Google Scholar]
  15. Maizels RM. Toxocara canis: molecular basis of immune recognition and evasion. Vet Parasitol 2013; 193:365–374 [View Article] [PubMed]
     [Google Scholar]
  16. Ma G, Luo Y, Zhu H, Luo Y, Korhonen PK et al. MicroRNAs of Toxocara canis and their predicted functional roles. Parasit Vectors 2016; 9:229 [View Article] [PubMed]
     [Google Scholar]
  17. Raissi V, Zibaei M, Raiesi O, Samani Z, Yarahmadi M et al. Parasite-derived microRNAs as a diagnostic biomarker: potential roles, characteristics, and limitations. J Parasit Dis 2021; 45:546–556 [View Article] [PubMed]
     [Google Scholar]
  18. Manzano-Román R, Siles-Lucas M. MicroRNAs in parasitic diseases: potential for diagnosis and targeting. Mol Biochem Parasitol 2012; 186:81–86 [View Article] [PubMed]
     [Google Scholar]
  19. Hakimi M, Cannella D. Apicomplexan parasites and subversion of the host cell microRNA pathway. Trends Parasitol 2011; 27:481–486 [View Article] [PubMed]
     [Google Scholar]
  20. Zhu X-Q, Korhonen PK, Cai H, Young ND, Nejsum P et al. Genetic blueprint of the zoonotic pathogen Toxocara canis. Nat Commun 2015; 6:1–8 [View Article] [PubMed]
     [Google Scholar]
  21. Rodríguez-Caballero A, Luna-Ochoa RI, Ponce-Macotela M, Peralata-Abarca GE, Martínez-Gordillo MN. A simple and inexpensive in vitro method for retrieving fertilized Toxocara canis eggs. Parasitol Res 2007; 101:829–832 [View Article] [PubMed]
     [Google Scholar]
  22. Fahrion AS, Schnyder M, Wichert B, Deplazes P. Toxocara eggs shed by dogs and cats and their molecular and morphometric species-specific identification: is the finding of T. cati eggs shed by dogs of epidemiological relevance. Vet Parasitol 2011; 177:186–189 [View Article] [PubMed]
     [Google Scholar]
  23. Olson LJ, Rose JE. Effect of Toxocara canis infection on the ability of white rats to solve maze problems. Exp Parasitol 1966; 19:77–84 [View Article] [PubMed]
     [Google Scholar]
  24. Tsui NBY, Ng EKO, Lo YMD. Stability of endogenous and added RNA in blood specimens, serum, and plasma. Clin Chem 2002; 48:1647–1653 [PubMed]
     [Google Scholar]
  25. Wang J, Czech B, Crunk A, Wallace A, Mitreva M et al. Deep small RNA sequencing from the nematode Ascaris reveals conservation, functional diversification, and novel developmental profiles. Genome Res 2011; 21:1462–1477 [View Article] [PubMed]
     [Google Scholar]
  26. Gevaert AB, Witvrouwen I, Vrints CJ, Heidbuchel H, Van Craenenbroeck EM et al. MicroRNA profiling in plasma samples using qPCR arrays: Recommendations for correct analysis and interpretation. PLoS One 2018; 13:e0193173 [View Article] [PubMed]
     [Google Scholar]
  27. Hastono SP. Analisis data kesehatan Depok: Fakultas Kesehatan Masyarakat Universitas Indonesia; 2007 p 217
     [Google Scholar]
  28. Pfaffl MW, Horgan GW, Dempfle L. Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 2002; 30:e36 [View Article] [PubMed]
     [Google Scholar]
  29. Rao X, Huang X, Zhou Z, Lin X. An improvement of the 2ˆ (–delta delta CT) method for quantitative real-time polymerase chain reaction data analysis. Biostat Bioinforma Biomath 2013; 3:71–85 [PubMed]
     [Google Scholar]
  30. Akao N, Chu AE, Tsukidate S, Fujita K. A rapid and sensitive screening kit for the detection of anti-Toxocara larval ES antibodies. Parasitol Int 1997; 46:189–195 [View Article]
     [Google Scholar]
  31. Zibaei M, Sadjjadi SM, Karamian M, Uga S, Oryan A et al. A comparative histopathology, serology and molecular study, on experimental ocular toxocariasis by Toxocara cati in Mongolian gerbils and Wistar rats. Biomed Res Int 2013; 2013:109580 [View Article] [PubMed]
     [Google Scholar]
  32. Raissi V, Mohebali M, Kia EB, Rahimi Foroushani A, Sohrabi N et al. Expression of Mir-21 and Mir-103a in Toxocara canis: potential for diagnosis of human toxocariasis. Iran J Parasitol 2020; 15:559–567 [View Article] [PubMed]
     [Google Scholar]
  33. Sommerfelt IE, Santillán G, Lopez C, Ribicich M, Franco AJ. Immunological and hematological response in experimental Toxocara canis-infected pigs. Vet Parasitol 2001; 96:127–134 [View Article] [PubMed]
     [Google Scholar]
  34. Ruiz-Manzano RA, Hernández-Cervantes R, Del Río-Araiza VH, Palacios-Arreola MI, Nava-Castro KE et al. Immune response to chronic Toxocara canis infection in a mice model. Parasite Immunol 2019; 41:e12672 [View Article] [PubMed]
     [Google Scholar]
  35. Özbakış G, Doğanay A. Visceral larva migrans detection using PCR–RFLP in BALB/c mice infected with Toxocara canis. J Helminthol 2019; 94: [View Article]
     [Google Scholar]
  36. Jarad N, Abbas AK, Aἀiz NN. Serodiagnosis of Toxocariasis by ELISA test using anti-T. canis IgG antibodies in stray dogs compared to PCR. IJVS 2019; 33:367–370 [View Article]
     [Google Scholar]
  37. Choudhuri S. Bioinformatics for Beginners: Genes, Genomes, Molecular Evolution, Databases and Analytical tools: Elsevier; 2014
     [Google Scholar]
  38. Glickman LT, Summers BA. Experimental Toxocara canis infection in cynomolgus macaques (Macaca fascicularis). Am J Vet Res 1983; 44:2347–2354 [PubMed]
     [Google Scholar]
  39. G TJ. Experimental Toxocara canis infections in chickens and pigeons. J Parasitol 1964; 50:124–127 [View Article] [PubMed]
     [Google Scholar]
  40. Scothorn MW, Koutz FR, Groves HF. Prenatal Toxocara canis infection in pups. J Am Vet Med Assoc 1965; 146:45–48 [PubMed]
     [Google Scholar]
  41. Dafalla A. A study of the effect of diethylcarbamazine and thiabendazole on experimental Toxocara canis infection in mice. J Trop Med Hyg 1972; 73:158–159
     [Google Scholar]
  42. Hay J, Arnott MA, Aitken PP, Kendall AT. Experimental toxocariasis and hyperactivity in mice. Z Parasitenkd 1986; 72:115–120 [View Article] [PubMed]
     [Google Scholar]
  43. Akao N, Tomoda M, Hayashi E, Suzuki R, Shimizu-Suganuma M et al. Cerebellar ataxia due to Toxocara infection in Mongolian gerbils, Meriones unguiculatus. Vet Parasitol 2003; 113:229–237 [View Article] [PubMed]
     [Google Scholar]
  44. Alba-Hurtado F, Muñoz-Guzmán MA, Valdivia-Anda G, Tórtora JL, Ortega-Pierres MG. Toxocara canis: larval migration dynamics, detection of antibody reactivity to larval excretory–secretory antigens and clinical findings during experimental infection of gerbils (Meriones unguiculatus). Exp Parasitol 2009; 122:1–5 [View Article] [PubMed]
     [Google Scholar]
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Changes in the expression of miR-103a and miR-21: a functional diagnosis of toxocariasis in rats
J. Med. Microbiol. 71, 001532 (2022); https://doi.org/10.1099/jmm.0.001532
/content/journal/jmm/10.1099/jmm.0.001532
/content/journal/jmm/10.1099/jmm.0.001532
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