1887

Abstract

is a bipolar Gram-negative bacillus and the causative agent of melioidosis; an infectious disease which commonly presents with bacteraemia.

Data regarding direct from blood culture identification of using the Vitek mass spectrometer (MS) are limited.

The authors aim to assess the safety and sensitivity of the Vitek MS for identification of from spiked positive blood culture samples.

Safety was assessed by determining the ability of the standard MS α-cyano-4-hydroxycinnamic acid (CHCA) matrix solution to inactivate . Organism identification using the manufacturer’s blood culture extraction method was compared to an in-house technique. Additionally, identification following abbreviated agar incubation of blood culture broth was performed.

All 70 MS target spots were inactivated by the matrix solution. The manufacturer’s blood culture extraction method identified 0/26 (0%) samples. An in-house method using the spun deposit from blood culture broth samples identified 38/38 (100%) samples. MS analysis of a blood culture broth drop on Chocolate agar following a 6 h incubation identified 30/32 (94%) samples.

Decreased time to diagnosis of melioidosis bacteraemia is likely to improve patient outcomes. This study adds to the literature with regards to the utility of MALDI-TOF MS identification of both directly from positive blood culture broth and a subsequent 6 h plate incubation. The use of a standard matrix solution inactivates the organism, and use of the spun deposit from a positive blood culture broth is most effective for early identification of .

Funding
This study was supported by the:
  • Pathology Queensland Study & Education Committee (Award SERC_6096)
    • Principle Award Recipient: IanGassiep
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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/content/journal/jmm/10.1099/jmm.0.001521
2022-04-22
2024-07-21
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