@article{mbs:/content/journal/jmm/10.1099/jmm.0.001519, author = "Shanmugakani, Rathina Kumar and Wu, Martin", title = "An isothermal amplification-coupled dipstick for the rapid detection of COVID-19", journal= "Journal of Medical Microbiology", year = "2022", volume = "71", number = "4", pages = "", doi = "https://doi.org/10.1099/jmm.0.001519", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001519", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "COVID-19", keywords = "reverse transcription helicase-dependent amplification", keywords = "dipstick", keywords = "SARS-CoV-2", eid = "001519", abstract = "Early detection of coronavirus disease 2019 (COVID-19) is critical for both initiating appropriate treatment and preventing the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent. A simple and rapid diagnostic test that can be performed without any expensive equipment would be valuable for clinicians working in a low-resource setting. Here, we report a point-of-care detection technique for COVID-19 that combines the power of isothermal amplification (reverse transcription helicase-dependent amplification, RT-HDA) and dipstick technologies. The limit of detection of this diagnostic test is six copies of SARS-CoV-2 µl−1 in clinical specimens. Of the 22 clinical specimens tested, RT-HDA-coupled dipstick correctly identified all positive and negative specimens. The RT-HDA can be performed over a heating block and the results can be interpreted visually with the dipstick technology without any specialized equipment. Furthermore, the RT-HDA-coupled dipstick could be performed in a short turnaround time of ~2 h. Thus, the RT-HDA-coupled dipstick could serve as a point-of-care diagnostic test for COVID-19 in a low-resource environment.", }