1887

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Volume 71, Issue 3

Predominance of community-associated, methicillin-susceptible infections among hospitalized children and adolescents No Access

Abstract

infections cause significant morbidity and mortality in children and adolescents.

There is limited data on the characteristics of infections requiring hospitalization in childhood.

To investigate the molecular epidemiology and antibiotic resistance of clinical isolates from children and adolescents.

All isolates recovered from patients aged <18 years, admitted to a referral hospital, with culture-proven invasive or non-invasive infections during the 4 year period 2015 to 2018 were analysed for antimicrobial resistance, virulence genes, PFGE and multilocus sequence typing (MLST). Cases were assigned to community-associated, community-onset healthcare-associated or hospital-associated infections based on epidemiological case definitions.

Among 139  infections, 88.5 % (123/139) were caused by methicillin-susceptible isolates (MSSA) and 73.4 % (102/139) were classified as community-associated infections. and genes were more common among MRSA as compared to MSSA isolates ( p 0.04; p 0.007). Invasive disease was noted in 22/139 patients (15.8 %). Staphylococcal scalded skin syndrome caused by fusidic-resistant MSSA increased over time (22.8 % in 2017–2018 vs 8.3 % in 2015–2016, OR 3.24; 95 % CI 1.10–8.36; 0.03). By PFGE genotyping, 22 pulsotypes were identified. A total of five sequence types (STs) were identified among 58 isolates analysed by MLST. More than one third of MSSA isolates (40/123, 32.5 %) and 13/23 (56.5 %) of SSSS isolates belonged to pulsotype 1, classified as sequence type 121 (ST121). MRSA isolates were equally distributed to pulsotypes A (ST30), B (ST239), C (ST80), H (ST225). ST121 isolates carried (40/40), genes (29/40), exhibited high resistance to fusidic acid and were increasingly resistant to mupirocin.

In our population, community-associated MSSA was the predominant cause of infections characterized by polyclonality, increasing resistance to fusidic acid and mupirocin. PFGE type 1 ST121 clone, harboured exfoliative toxin genes and was associated with rising trends of SSSS.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): MRSA , PVL , SSSS , ST121 , Staphylococcus aureus and virulence
Funding
This study was supported by the:
  • European Society for Paediatric Infectious Disease (Award ESPID Small Grant 2017)
    • Principle Award Recipient: MARIATSIRIGOTAKI
© 2022 The Authors
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2022-03-31
2024-04-18
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Predominance of community-associated, methicillin-susceptible Staphylococcus aureus infections among hospitalized children and adolescents
J. Med. Microbiol. 71, 001511 (2022); https://doi.org/10.1099/jmm.0.001511
/content/journal/jmm/10.1099/jmm.0.001511
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