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Volume 71, Issue 3

Localized outbreaks of belonging to international high-risk clones in a south Indian hospital No Access

Abstract

. is now considered as a major bacterial pathogen associated with hospital infections. Frequently, multidrug-resistant (MDR) and extensively drug-resistant (XDR) are being encountered. Unusual increase in the infections led to the suspicion of outbreaks in the urology ward and cardiothoracic and vascular surgery intensive care unit (CTVS-ICU).

We hypothesize that the localized outbreaks may have originated from environmental sources within the hospital premises. An alternative possibility is the transmission from a previously infected patient or hospital attendant. Understanding the drug-resistance profile and genome characteristics of these clinical samples would determine the likely source of infection and spread.

To perform epidemiological and molecular investigations on the suspected outbreaks of in the study centre and identify potential sources of infection.

Fourteen drug-resistant isolated from patients of the urology ward, CTVS-ICU and tap waters collected during the suspected outbreaks were subjected to microbiological and genomic analysis. Comparative genome (CG) analysis of these 14 study genomes with 284 complete genomes was performed.

Multilocus sequence typing analysis revealed that the isolates belonged to five different sequence types (ST235, ST357, ST639, ST654 and ST1203) and clustered into three distinct groups while two CTVS-ICU isolates remained as singletons. Genome analysis distinguished that the outbreaks in the urology ward and CTVS-ICU are independent, epidemiologically unrelated to each other and with the tap-water isolates.

This study highlights the presence of distinct, clonally unrelated, drug-resistant within a hospital setting. The genome analysis of the two localized outbreaks revealed their distinct genetic background and phylogenetically unrelated origin. Vigilant screening and effective implementation of infection control measures led to the successful containment of potential environmental reservoirs of within the premises.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antibiotic resistance , biofilm , global high-risk clones , hospital outbreak , Pseudomonas aeruginosa and whole-genome sequencing
Funding
This study was supported by the:
  • Indian Council of Medical Research (Award OMI/27/2020-ECD-I)
    • Principle Award Recipient: BulagondaEswarappa Pradeep
© 2022 The Authors
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2022-03-14
2024-10-09
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Localized outbreaks of Pseudomonas aeruginosa belonging to international high-risk clones in a south Indian hospital
J. Med. Microbiol. 71, 001500 (2022); https://doi.org/10.1099/jmm.0.001500
/content/journal/jmm/10.1099/jmm.0.001500
/content/journal/jmm/10.1099/jmm.0.001500
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