1887

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Volume 70, Issue 12

Development of a nucleic acid chromatography assay for the detection of commonly isolated rapidly growing mycobacteria No Access

Abstract

Non-tuberculosis mycobacterium infections are increasing worldwide, including those caused by rapidly growing mycobacteria (RGM).

The identification of the aetiological agent in the context of infections is essential for the adoption of an adequate therapeutic approach. However, the methods for the rapid distinction of different RGM species are less than optimal.

To develop a nucleic acid chromatography kit to identify clinically common RGM.

We tried to develop a nucleic acid chromatography kit designed to detect four RGM species (including three subspecies) i.e. subsp. , subsp. (detected as ) subsp. , , and . The amplified target genes for each species/subspecies using multiplex PCR were analysed using a nucleic acid chromatography assay.

Among the 159 mycobacterial type strains and 70 RGM clinical isolates tested, the developed assay correctly identified all relevant RGM without any cross-reactivity or false-negatives. The limits of detection for each species were approximately 0.2 pg µl.

The rapid and simple nucleic acid chromatography method developed here, which does not involve heat denaturation, may contribute to the rapid identification and treatment of RGM infections.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Mycobacterium abscessus , Mycobacterium bolletii , Mycobacterium fortuitum , Mycobacterium massiliense , nucleic acid chromatography and rapidly growing mycobacteria (RGM)
Funding
This study was supported by the:
  • Japan Agency for Medical Research and Development (Award 19fk0108043h0403)
    • Principle Award Recipient: SatoshiMitarai
© 2021 The Authors
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/content/journal/jmm/10.1099/jmm.0.001464
2021-12-08
2024-04-19
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Development of a nucleic acid chromatography assay for the detection of commonly isolated rapidly growing mycobacteria
J. Med. Microbiol. 70, 001464 (2021); https://doi.org/10.1099/jmm.0.001464
/content/journal/jmm/10.1099/jmm.0.001464
/content/journal/jmm/10.1099/jmm.0.001464
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