1887

Journal of Medical Microbiology logo

Volume 70, Issue 10

Multicenter evaluation of the FilmArray Meningitis/Encephalitis assay in a routine setting No Access

Abstract

The FilmArray Meningitis/Encephalitis (FA-ME) Panel (Biofire, Salt Lake City, Utah, US) enables fast and automated detection of 14 pathogens in cerebrospinal fluid (CSF).

The performance of the FA-ME panel in a real routine setting has not yet been described and could lead to better patient management in cases of good performance.

This multicenter study verified the FA-ME panel analytical performance in a routine hospital setting.

Between April 2016 and April 2018, 454 CSF samples were analysed with the FA-ME panel and compared with routine diagnostics. In cases of discrepancy or lack of a comparator result, a profound analysis based on patient records and other laboratory results was performed.

A first analysis of 65 frozen samples, suspicious for meningitis had a 89 % concordance with routine diagnostics. The limit of detection (LOD) was confirmed for all pathogens except for and a strain of and LOD experiments were not performed). The routine evaluation showed a positive result in 114 (25 %) clinical samples for at least one target. In three samples co-infections were found. After discrepancy analysis, overall sensitivity was 98 % (false negative FA-ME results for one HSV2, two HSV1 and two parechovirus). Four FA-ME results were considered false positive (two HHV6, one VZV and one ), resulting in an overall specificity of >99 %. A clinical added value of the assay was seen in the diagnosis of eight cases of bacterial meningitis.

Because of its rapidity and ease of use, the FA-ME panel has great potential in the diagnosis of central nervous infections. Implementation can improve clinical management, but costs and analytical limitations need to be addressed to convince clinicians and laboratories of its value.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Biofire , encephalitis , FilmArray® , meningitis and multiplex PCR (syndromic)
© 2021 The Authors
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/content/journal/jmm/10.1099/jmm.0.001438
2021-10-19
2024-04-23
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References

  1. Brouwer MC, Tunkel AR, van de Beek D. Epidemiology, diagnosis, and antimicrobial treatment of acute bacterial meningitis. Clin Microbiol Rev 2010; 23:467–492 [View Article] [PubMed]
     [Google Scholar]
  2. Garcia LS. Clinical Microbiology Procedures Handbook, 3rd edn. Washington: ASM Press; 2010
     [Google Scholar]
  3. Green DA, Pereira M, Miko B, Radmard S, Whittier S et al. Clinical significance of human herpesvirus 6 positivity on the Filmarray meningitis/encephalitis panel. Clin Infect Dis 2018; 67:1125–1128 [View Article] [PubMed]
     [Google Scholar]
  4. Graf EH, Farquharson MV, Cárdenas AM. Comparative evaluation of the filmarray meningitis/encephalitis molecular panel in a pediatric population. Diagn Microbiol Infect Dis 2017; 87:92–94 [View Article] [PubMed]
     [Google Scholar]
  5. Leber AL, Everhart K, Balada-Llasat J-M, Cullison J, Daly J et al. Multicenter evaluation of Biofire Filmarray Meningitis/encephalitis panel for detection of bacteria, viruses, and yeast in cerebrospinal fluid specimens. J Clin Microbiol 2016; 54:2251–2261 [View Article] [PubMed]
     [Google Scholar]
  6. Messacar K, Breazeale G, Robinson CC, Dominguez SR. Potential clinical impact of the film array meningitis encephalitis panel in children with suspected central nervous system infections. Diagn Microbiol Infect Dis 2016; 86:118–120 [View Article] [PubMed]
     [Google Scholar]
  7. Dien Bard J, Alby K. Point-counterpoint: Meningitis/encephalitis syndromic testing in the clinical laboratory. J Clin Microbiol 2018; 56:
     [Google Scholar]
  8. Hanson KE. The first fully automated molecular diagnostic panel for meningitis and encephalitis: How well does it perform, and when should it be used?. J Clin Microbiol 2016; 54:2222–2224 [View Article] [PubMed]
     [Google Scholar]
  9. Soucek DK, Dumkow LE, VanLangen KM, Jameson AP. Cost justification of the Biofire Filmarray Meningitis/encephalitis panel versus standard of care for diagnosing meningitis in a community hospital. J Pharm Pract 2019; 32:36–40 [View Article] [PubMed]
     [Google Scholar]
  10. Verbeeck J, Van Kerschaver E, Wollants E, Beuselinck K, Stappaerts L et al. Detection of perinatal cytomegalovirus infection and sensorineural hearing loss in belgian infants by measurement of automated auditory brainstem response. J Clin Microbiol 2008; 46:3564–3568 [View Article] [PubMed]
     [Google Scholar]
  11. Watzinger F, Suda M, Preuner S, Baumgartinger R, Ebner K et al. Real-time quantitative PCR assays for detection and monitoring of pathogenic human viruses in immunosuppressed pediatric patients. J Clin Microbiol 2004; 42:5189–5198 [View Article] [PubMed]
     [Google Scholar]
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Multicenter evaluation of the FilmArray Meningitis/Encephalitis assay in a routine setting
J. Med. Microbiol. 70, 001438 (2021); https://doi.org/10.1099/jmm.0.001438
/content/journal/jmm/10.1099/jmm.0.001438
/content/journal/jmm/10.1099/jmm.0.001438
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