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Volume 70, Issue 10

Quinolone-resistant uropathogenic : is there a relation between genes, gene target site mutation and biofilm formation? No Access

Abstract

The resistance to quinolone reported in uropathogenic (UPEC) is commonly caused by mutations in the target site encoding genes such as the gene. Bacterial plasmids carrying resistance genes such as genes can also transfer resistance. Biofilms produced by UPEC can further aid the development of resistant urinary tract infections (UTIs).

Biofilm production is associated with higher prevalence of quinolones resistance genetic determinants.

To detect the prevalence of genes and gene mutation among quinolone-resistant UPEC and to investigate the relation between these genetic resistance determinants and biofilm production.

Catheterized urine samples were collected from 420 patients with evidence of UTIs and processed using standard techniques. Isolated UPEC were screened for quinolone resistance using an antimicrobial susceptibility test. Biofilm production among quinolone-resistant isolates was detected using the tissue culture plate method. All quinolone-resistant isolates were screened for genes ( and ) by multiplex PCR and for gene mutation by PCR-RFLP.

Two hundred and sixty-four UPEC isolates were detected from 420 processed urine samples. Out of the identified 264 UPEC 123 (46.6 %) isolates were found to be quinolone-resistant, showing resistance to 1 or more of the tested quinolones. Of the 123 quinolone-resistant UPEC detected, 71(57.7 %) were biofilm producers. The genes were detected among 62.6 % of the quinolone-resistant UPEC, with an estimated prevalence of 22.8 32.5 and 37.4 % for and genes, respectively. Additionally, the gene mutation was identified among 53.7 % of the quinolone-resistant isolates. We reported a significant association between biofilm production and the presence of , and genes. Furthermore, the gene mutation was significantly associated with biofilm-producing isolates. The coexistence of genes, gene mutation and biofilm production was demonstrated in almost 40 % of the quinolone-resistant isolates.

A significantly higher prevalence of genes ( and ) as well as the gene mutation was found among biofilm-forming UPEC. The reported coexistence of these different resistance mechanisms could aggravate quinolone resistance. Therefore, monitoring of resistance mechanisms and a proper stewardship programme are necessary.

  • Received:
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Keyword(s): biofilm , catheterized patients , quinolone resistance and uropathogenic E. coli
© 2021 The Authors
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2021-10-19
2024-04-19
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Quinolone-resistant uropathogenic E. coli: is there a relation between qnr genes, gyrA gene target site mutation and biofilm formation?
J. Med. Microbiol. 70, 001432 (2021); https://doi.org/10.1099/jmm.0.001432
/content/journal/jmm/10.1099/jmm.0.001432
/content/journal/jmm/10.1099/jmm.0.001432
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