1887

Journal of Medical Microbiology logo

Volume 70, Issue 9

Analysis of the antimicrobial resistance gene frequency in whole-genome sequenced from Latin American countries No Access

Abstract

species are important environmental-related bacteria responsible for diverse infections in humans due to consumption of contaminated water and seafood in underdeveloped areas of the world. This study aimed to investigate the frequency of antimicrobial resistance genes in 577 sequenced and strains isolated in Latin American countries available at the NCBI Pathogen Detection database and to determine the sequence type (ST) of the strains. Almost all strains studied (99.8%) carried at least one antimicrobial resistance gene, while 54.2 % presented a multidrug-resistance profile. The strains exhibited genotypic resistance to 11 antimicrobial classes and , and , which confer resistance to antibiotic peptides, β-lactams and amphenicols, respectively, were the most detected genes. and showed a broad diversity of STs. strains isolated in Haiti after 2010’s earthquake presented the highest diversity and amount of resistance genes in the set of strains analysed and mostly belonged to ST69. In conclusion, the detection of resistance genes from 11 antimicrobial classes and the high number of multidrug-resistant species strains emphasize that Latin American public health authorities should employ more efficient control measures and that special attention should be given for the rational use of antimicrobials in human therapy and aquaculture, since the consumption of contaminated water and seafood with resistant may result in human infections difficult to be treated.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antimicrobial resistance , cholera , environmental bacteria , resistance gene content and Vibrio
Funding
This study was supported by the:
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Award 001)
    • Principle Award Recipient: FelipePinheiro Vilela
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (Award 304399/2018-3)
    • Principle Award Recipient: JulianaPfrimer Falcao
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (Award 2019/06947-6)
    • Principle Award Recipient: FelipePinheiro Vilela
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (Award 2019/19338-8)
    • Principle Award Recipient: JulianaPfrimer Falcao
© 2021 The Authors
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.001428
2021-09-29
2024-04-25
Loading full text...

Full text loading...

References

  1. O’Neill J. Antimicrobial Resistance: Tackling a crisis for the health and wealth of nations. The Review on Antimicrobial Resistance Chaired by Jim O’Neill; 2014 https://amr-review.org/sites/default/files/AMR%20Review%20Paper%20-%20Tackling%20a%20crisis%20for%20the%20health%20and%20wealth%20of%20nations_1.pdf
  2. Baquero F, Martínez JL, Cantón R. Antibiotics and antibiotic resistance in water environments. Curr Opin Biotechnol 2008; 19:260–265 [View Article] [PubMed]
     [Google Scholar]
  3. Cabello FC, Godfrey HP, Tomova A, Ivanova L, Dölz H et al. Antimicrobial use in aquaculture re-examined: its relevance to antimicrobial resistance and to animal and human health. Environ Microbiol 2013; 15:1917–1942 [View Article] [PubMed]
     [Google Scholar]
  4. Osunla CA, Okoh AI. Vibrio pathogens: a public health concern in rural water resources in Sub-Saharan Africa. Int J Environ Res Public Health 2017; 14:10 [View Article] [PubMed]
     [Google Scholar]
  5. Baker-Austin C, Oliver JD, Alam M, Ali A, Waldor MK et al. Vibrio spp. infections. Nat Rev Dis Primers 2018; 4:8 [View Article] [PubMed]
     [Google Scholar]
  6. Janda JM, Newton AE, Bopp CA. Vibriosis. Clin Lab Med 2015; 35:273–288 [View Article] [PubMed]
     [Google Scholar]
  7. Centers for Disease Control and Prevention (CDC) Antibiotic Treatment - Recommendations for the Use of Antibiotics for the Treatment of Cholera. Cholera - Vibrio cholerae infection; 2020 https://www.cdc.gov/cholera/treatment/antibiotic-treatment.html#summary
  8. Centers for Disease Control and Prevention (CDC) Information for Health Professionals & Laboratorians. Vibrio Species Causing Vibriosis; 2020 https://www.cdc.gov/vibrio/healthcare.html
  9. Velazquez-Roman J, León-Sicairos N, de Jesus Hernández-Díaz L, Canizalez-Roman A. Pandemic Vibrio parahaemolyticus O3:K6 on the American continent. Front Cell Infect Microbiol 2014; 3:110 [View Article] [PubMed]
     [Google Scholar]
  10. Raszl SM, Froelich BA, Vieira CR, Blackwood AD, Noble RT. Vibrio parahaemolyticus and Vibrio vulnificus in South America: water, seafood and human infections. J Appl Microbiol 2016; 121:1201–1222 [View Article] [PubMed]
     [Google Scholar]
  11. Piarroux R, Faucher B. Cholera epidemics in 2010: respective roles of environment, strain changes, and human-driven dissemination. Clin Microbiol Infect 2012; 18:231–238 [View Article] [PubMed]
     [Google Scholar]
  12. Sigman M, Luchette FA. Cholera: something old, something new. Surg Infect 2012; 13:216–222 [View Article]
     [Google Scholar]
  13. Moran-Gilad J. whole genome sequencing (WGS) for food-borne pathogen surveillance and control - taking the pulse. Euro Surveill 2017; 22: [View Article] [PubMed]
     [Google Scholar]
  14. Kan B, Zhou H, Du P, Zhang W, Lu X. Transforming bacterial disease surveillance and investigation using whole-genome sequence to probe the trace. Front Med 2018; 12:23–33 [View Article] [PubMed]
     [Google Scholar]
  15. Allard MW, Bell R, Ferreira CM, Gonzalez-Escalona N, Hoffmann M. Genomics of foodborne pathogens for microbial food safety. Curr Opin Biotechnol 2018; 49:224–229 [View Article] [PubMed]
     [Google Scholar]
  16. Alcock BP, Raphenya AR, Lau TTY, Tsang KK, Bouchard M. CARD 2020: antibiotic resistome surveillance with the comprehensive antibiotic resistance database. Nucleic Acids Res 2020; 48:D517–D525 [View Article] [PubMed]
     [Google Scholar]
  17. Larsen MV, Cosentino S, Rasmussen S, Friis C, Hasman H et al. Multilocus sequence typing of total-genome-sequenced bacteria. J Clin Microbiol 2012; 50:1355–1361 [View Article] [PubMed]
     [Google Scholar]
  18. Dauros P, Bello H, Domínguez M, Hormazábal JC, González G. Characterization of Vibrio parahaemolyticus strains isolated in Chile in 2005 and in 2007. J Infect Dev Ctries 2011; 5:502–510 [View Article] [PubMed]
     [Google Scholar]
  19. Rebouças RH, de Sousa OV, Lima AS, Vasconcelos FR, de Carvalho PB. Antimicrobial resistance profile of Vibrio species isolated from marine shrimp farming environments (Litopenaeus vannamei) at Ceará, Brazil. Environ Res 2011; 111:21–24 [View Article] [PubMed]
     [Google Scholar]
  20. Sjölund-Karlsson M, Reimer A, Folster JP, Walker M, Dahourou GA. Drug-resistance mechanisms in Vibrio cholerae O1 outbreak strain, Haiti, 2010. Emerg Infect Dis 2011; 17:2151–2154 [View Article] [PubMed]
     [Google Scholar]
  21. Talkington D, Bopp C, Tarr C, Parsons MB, Dahourou G. Characterization of toxigenic Vibrio cholerae from Haiti, 2010-2011. Emerg Infect Dis 2011; 17:2122–2129 [View Article] [PubMed]
     [Google Scholar]
  22. Aedo S, Ivanova L, Tomova A, Cabello FC. Plasmid-related quinolone resistance determinants in epidemic Vibrio parahaemolyticus, uropathogenic Escherichia coli, and marine bacteria from an aquaculture area in Chile. Microb Ecol 2014; 68:324–328 [View Article] [PubMed]
     [Google Scholar]
  23. Rocha RoS, Sousa OV, Vieira RH. Multidrug-resistant Vibrio associated with an estuary affected by shrimp farming in Northeastern Brazil. Mar Pollut Bull 2016; 105:337–340 [View Article] [PubMed]
     [Google Scholar]
  24. Silva IP, Carneiro CdS, Saraiva MAF, de Oliveira TAS, de Sousa OV. Antimicrobial resistance and potential virulence of Vibrio parahaemolyticus isolated from water and bivalve mollusks from Bahia, Brazil. Mar Pollut Bull 2018; 131:757–762 [View Article] [PubMed]
     [Google Scholar]
  25. Sulca MA, Orozco R, Alvarado DE. Antimicrobial resistance not related to 1,2,3 integrons and Superintegron in Vibrio spp. isolated from seawater sample of Lima (Peru. Mar Pollut Bull 2018; 131:370–377 [View Article] [PubMed]
     [Google Scholar]
  26. Arteaga M, Velasco J, Rodriguez S, Vidal M, Arellano C. Genomic characterization of the non-O1/non-O139 Vibrio cholerae strain that caused a gastroenteritis outbreak in Santiago, Chile, 2018. Microb Genom 2020; 6:e000340 [View Article] [PubMed]
     [Google Scholar]
  27. Tendencia EA, de la Peña LD. Antibiotic resistance of bacteria from shrimp ponds. Aquaculture 2001; 195:193–204 [View Article]
     [Google Scholar]
  28. Martínez JL. Antibiotics and antibiotic resistance genes in natural environments. Science 2008; 321:365–367 [View Article] [PubMed]
     [Google Scholar]
  29. Coutinho FH, Silveira CB, Pinto LH, Salloto GR, Cardoso AM et al. Antibiotic resistance is widespread in urban aquatic environments of Rio de Janeiro. Brazil Microb Ecol 2014; 68:441–452 [View Article]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.001428
Loading
Analysis of the antimicrobial resistance gene frequency in whole-genome sequenced Vibrio from Latin American countries
J. Med. Microbiol. 70, 001428 (2021); https://doi.org/10.1099/jmm.0.001428
/content/journal/jmm/10.1099/jmm.0.001428
/content/journal/jmm/10.1099/jmm.0.001428
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error