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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 70, Issue 8

Emergence of a multidrug-resistant plasmid encoding , and in a clinical isolate of in Japan No Access

Abstract

(formerly genospecies 15 Tjernberg and Ursing) has been isolated from humans and animals and was proposed to be a novel species in 2015. A multidrug-resistant isolate, RYU24, was obtained in 2012 from an inpatient in Okinawa, Japan, with no record of overseas travel. The isolate was resistant to carbapenems, aminoglycosides and ciprofloxacin, with minimum inhibitory concentrations (MICs) of 32 µg ml for imipenem and meropenem; > 1024 µg ml for amikacin, arbekacin, gentamicin and tobramycin; and 8 µg ml for ciprofloxacin. The isolate was found to harbour a 68-kbp plasmid carrying , which encodes New Delhi metallo-β-lactamase-1 (NDM-1); , which encodes an OXA-58-like carbapenemase and; , which encodes ArmA 16S rRNA methylase conferring pan-aminoglycoside resistance. To our knowledge, this is the first report of a plasmid harbouring the three major drug-resistance genes, , and .

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Keyword(s): Acinetobacter variablis , ArmA , carbapenem inactivation method (CIMTris II) , carbapenemase , NDM-1 and OXA-420
Funding
This study was supported by the:
  • the JU Research Fund (Keiko Yamazaki)
    • Principle Award Recipient: KoheiUechi
  • Japan Agency for Medical Research and Development (Award 20fk0108061h0303)
    • Principle Award Recipient: KoheiUechi
  • Japan Society for the Promotion of Science (Award 19KK0203)
    • Principle Award Recipient: TeruoKirikae
  • Japan Society for the Promotion of Science (Award 19K16652)
    • Principle Award Recipient: MariTohya
  • Japan Society for the Promotion of Science (Award 18K07121)
    • Principle Award Recipient: TeruoKirikae
  • Japan Society for the Promotion of Science (Award 18K07120)
    • Principle Award Recipient: TatsuyaTada
© 2021 The Authors
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/content/journal/jmm/10.1099/jmm.0.001395
2021-08-25
2025-01-23
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Emergence of a multidrug-resistant plasmid encoding blaNDM-1, blaOXA-420 and armA in a clinical isolate of Acinetobacter variabilis in Japan
J. Med. Microbiol. 70, 001395 (2021); https://doi.org/10.1099/jmm.0.001395
/content/journal/jmm/10.1099/jmm.0.001395
/content/journal/jmm/10.1099/jmm.0.001395
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