1887

Abstract

New Delhi metallo-β-lactamase (NDM)-producing has become a serious global health concern.

Due to the high genetic diversity among NDM-positive we need further surveillance and studies to better understand the relationships between them. In addition, the coexistence of several plasmid replicon types in NDM-positive may affect the copy number of , the MIC level to antibiotics, as well as increasing the chance of horizontal gene transfer.

The aim of this study was to determine incompatible plasmid groups and copy numbers of , and to investigate the genetic relationship of 37 NDM-positive in Kerman, Iran.

The gene was detected and confirmed by PCR-sequencing. The plasmid replicon types were determined by PCR-based replicon typing (PBRT) and the copy number of was determined by quantitaive real time-PCR (qPCR). Random amplified polymorphic DNA (RAPD)-PCR typing was used to detect genetic relationships between the strains.

In this study, 10 different replicon types, including Frep [=25 (67.5 %)], FIIAs [=11 (29.7 %)], FIA [=5 (13.5 %)], FIB [=3 (8.1 %)], I1-Iγ [=2 (5.4 %)], L/M [=7 (18.9 %)], A/C [=7 (18.9 %)], Y [=3 (8.1 %)], P [=1 (2.7 %)] and FIC [=1 (2.7 %)] were reported. The copy numbers of the gene varied from 30.00 to 5.0×10 and no statistically significant correlation was observed between a rise of the MIC to imipenem and the copy numbers of (>0.05). According to RAPD typing results, 35 strains were divided into five clusters, while two strains were non-typeable.

The spread of NDM-1-producing strains that carry several plasmid replicon types increases the chance of horizontal transfer of antibiotic resistance genes in hospital settings. In this study, 10 different replicon types were identified. We could not find any relationship between the increase of MIC levels to imipenem and the copy numbers of . Therefore, due to the identification of different replicon types in this study, the type and genetic characteristics of -carrying plasmids, and other factors such as antibiotic selective pressure, probably affect the copy number of and change the MIC level to imipenem.

Funding
This study was supported by the:
  • Kerman University of Medical Sciences (Award 96000823 and 98001031)
    • Principle Award Recipient: DavoodKalantar-Neyestanaki
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/content/journal/jmm/10.1099/jmm.0.001361
2021-05-17
2024-03-29
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