1887

Abstract

subspecies (SDSE) is becoming increasingly recognized as an important human pathogen. Recurrent bacteremia with SDSE has been described previously.

The aims of the study were to establish the genetic relatedness of SDSE isolates with -type stG643 that had caused recurrent bacteraemia in three patients and to search for signs of horizontal gene transfer of the gene in a collection of SDSE stG643 genomes.

Recurring SDSE bacteremia is caused by the same clone in one patient.

Whole genome sequencing of 22 clinical SDSE stG643 isolates was performed, including three paired blood culture isolates and sixteen isolates from various sites. All assemblies were aligned to a reference assembly and SNPs were extracted. A total of 53 SDSE genomes were downloaded from GenBank. Two phylogenetic trees, including all 75 SDSE isolates, were created. One tree was based on the gene only and one tree was based on all variable positions in the genomes.

The genomes from the three pairs of SDSE isolates showed high sequence similarity (1–17 SNPs difference between the pairs), whereas the median SNP difference between the 22 isolates in our collection was 1694 (range 1–11257). The paired isolates were retrieved with 7–53 months between episodes. The 22 SDSE isolates from our collection formed a cluster in the phylogenetic tree based on the gene, while they were more scattered in the tree based on all variable positions.

Our results show that the paired isolates were of the same clonal origin, which in turn supports carriage between bacteraemia episodes. The phylogenetic analysis indicates that horizontal gene transfer of the gene between some of the SDSE isolates has occurred.

Funding
This study was supported by the:
  • MagnusRasmussen , Swedish governmental funding of clinical research within the NHS , (Award 2018-Projekt0253)
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/content/journal/jmm/10.1099/jmm.0.001330
2021-02-22
2021-03-08
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References

  1. Bramhachari PV, Kaul SY, McMillan DJ, Shaila MS, Karmarkar MG et al. Disease burden due to Streptococcus dysgalactiae subsp. equisimilis (group G and C Streptococcus) is higher than that due to Streptococcus pyogenes among Mumbai school children. J Med Microbiol 2010; 59: 220 223 [CrossRef] [PubMed]
    [Google Scholar]
  2. Rantala S, Vuopio-Varkila J, Vuento R, Huhtala H, Syrjänen J. Clinical presentations and epidemiology of beta-haemolytic streptococcal bacteraemia: a population-based study. Clin Microbiol Infect 2009; 15: 286 288 [CrossRef] [PubMed]
    [Google Scholar]
  3. Takahashi T, Ubukata K, Watanabe H. Invasive infection caused by Streptococcus dysgalactiae subsp. equisimilis: characteristics of strains and clinical features. J Infect Chemother 2011; 17: 1 10 [CrossRef] [PubMed]
    [Google Scholar]
  4. Rantala S. Streptococcus dysgalactiae subsp. equisimilis bacteremia: an emerging infection. Eur J Clin Microbiol Infect Dis 2014; 33: 1303 1310 [CrossRef] [PubMed]
    [Google Scholar]
  5. Rantala S, Tuohinen S. Two cases of cardiac device-related endocarditis due to Streptococcus dysgalactiae subsp. equisimilis (group C or G streptococci). BMC Infect Dis 2014; 14: 174 [CrossRef] [PubMed]
    [Google Scholar]
  6. Liao C-H, Liu L-C, Huang Y-T, Teng L-J, Hsueh P-R. Bacteremia caused by group G streptococci, Taiwan. Emerg Infect Dis 2008; 14: 837 840 [CrossRef] [PubMed]
    [Google Scholar]
  7. Sylvetsky N, Raveh D, Schlesinger Y, Rudensky B, Yinnon AM. Bacteremia due to beta-hemolytic Streptococcus group G: increasing incidence and clinical characteristics of patients. Am J Med 2002; 112: 622 626 [CrossRef] [PubMed]
    [Google Scholar]
  8. Rantala S, Vahakuopus S, Vuopio-Varkila J, Vuento R, Syrjanen J. Streptococcus dysgalactiae subsp. equisimilis bacteremia, Finland, 1995-2004. Emerg Infect Dis 2010; 16: 843 846 [CrossRef] [PubMed]
    [Google Scholar]
  9. Cohen-Poradosu R, Jaffe J, Lavi D, Grisariu-Greenzaid S, Nir-Paz R et al. Group G streptococcal bacteremia in Jerusalem. Emerg Infect Dis 2004; 10: 1455 1460 [CrossRef] [PubMed]
    [Google Scholar]
  10. Tee WSN, Lieu PK, Ngan CCL. Epidemiology of beta-haemolytic group G streptococcal bacteraemia in Singapore (1996 to 1998). Ann Acad Med Singap 2002; 31: 86 91 [PubMed]
    [Google Scholar]
  11. Trell K, Sendi P, Rasmussen M. Recurrent bacteremia with Streptococcus dysgalactiae: a case-control study. Diagn Microbiol Infect Dis 2016; 85: 121 124 [CrossRef] [PubMed]
    [Google Scholar]
  12. Fujita T, Yoshida H, Osaka S, Hirose Y, Goto M et al. Comparison of characteristics of Streptococcus dysgalactiae subsp. equisimilis isolates causing repetitive vs single infections. Ann Lab Med 2019; 39: 488 492 [CrossRef] [PubMed]
    [Google Scholar]
  13. Suzuki K, Nakamura A, Ishikura K, Imai H. Recurrent SDSE bacteraemia resulting in streptococcal toxic shock syndrome in a patient with Noonan syndrome. BMJ Case Rep 2016; 2016: bcr2016216092 [CrossRef]
    [Google Scholar]
  14. Trell K, Nilson B, Rasmussen M. Species and emm-type distribution of group C and G streptococci from different sites of isolation. Diagn Microbiol Infect Dis 2016; 86: 467 469 [CrossRef] [PubMed]
    [Google Scholar]
  15. McMillan DJ, Bessen DE, Pinho M, Ford C, Hall GS et al. Population genetics of Streptococcus dysgalactiae subspecies equisimilis reveals widely dispersed clones and extensive recombination. PLoS One 2010; 5: e11741 [CrossRef] [PubMed]
    [Google Scholar]
  16. Ahmad Y, Gertz RE, Li Z, Sakota V, Broyles LN et al. Genetic relationships deduced from emm and multilocus sequence typing of invasive Streptococcus dysgalactiae subsp. equisimilis and S. canis recovered from isolates collected in the United States. J Clin Microbiol 2009; 47: 2046 2054 [CrossRef] [PubMed]
    [Google Scholar]
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