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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 70, Issue 3

Co-infections of two carbapenemase-producing clinical strains isolated from the same diabetes individual in China No Access

Abstract

Since was first reported in China, there have been ten variants of MCR appearing nationwide so far. Multidrug-resistant bacteria carrying both NDM and MCR have become a serious threat to global public health.

The genetic structure of needs to be better understood in order to better prevent and control the transmission of drug-resistant genes.

The aim of this study was to characterize the presence of two isolates, which carries CME2 and the coexistence of and strain CMD2, which were isolated from a patient with diabetes in Sichuan, China.

The microbroth dilution method was used for antibiotic susceptibility. Conjugation experiment was used to investigate the transferability of , and . Whole-genome sequencing was performed on Illumina HiSeq platform. The ability of biofilm formation was detected by crystal-violet staining, the virulence of the bacteria was measured by killing assay.

carrier CME2 and CMD2 with and were resistant to carbapenems, β-lactam, aminoglycoside, quinolone and tetracycline, while CMD2 was also resistant to colistin. Conjugation assay and plasmid replicon typing further demonstrated that both and were respectively present on the self-transferrable IncX3 plasmid, was located on the self-transferrable IncHI2 plasmid. Through the analysis of gene context, the structure was -IS-Δ-IS, context was IS-ΔIS-IS --ΔIS structure, structure was IS --ΔIS-IS. Biofilm formation of CME2 was stronger than CMD2. There was no significant difference in virulence between the two strains.

This study reveals two multiple drug-resistant isolates from diabetes patient samples. carrying two NDM-resistant genes is already a serious threat, where MCR is an important cause of treatment failure in bacterial infections. This study is a reminder not only to prevent infection in patients with diabetes, but also to constantly monitor the epidemic and spread of the drug-resistant gene.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): diabetes , Enterobacter hormaechei , mcr-9 and Whole-genome sequencing
Funding
This study was supported by the:
  • United Funds of Luzhou and Southwest Medical University (Award 2018LZXNYD-ZK51)
    • Principle Award Recipient: ManlinDing
  • the Application Technology Research and Development of Sichuan Science and Technology (Award 20YYJC0952)
    • Principle Award Recipient: NotApplicable
© 2021 The Authors
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/content/journal/jmm/10.1099/jmm.0.001316
2021-02-02
2025-03-19
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/content/journal/jmm/10.1099/jmm.0.001316
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Co-infections of two carbapenemase-producing Enterobacter hormaechei clinical strains isolated from the same diabetes individual in China
J. Med. Microbiol. 70, 001316 (2021); https://doi.org/10.1099/jmm.0.001316
/content/journal/jmm/10.1099/jmm.0.001316
/content/journal/jmm/10.1099/jmm.0.001316
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