1887

Abstract

Onychomycosis infections currently show a significant increase, affecting about 10 % of the world population. is the main agent responsible for about 80 % of the reported infections. The clinical cure for onychomycosis is extremely difficult and effective new antifungal therapy is needed.

onychomycosis models using porcine hooves can be an excellent alternative for evaluating the efficacy of new anti-dermatophytic agents in a nail lacquer.

Evaluation of the effectiveness of a nail lacquer containing a quinoline derivative on an onychomycosis model using porcine hooves, as well as the proposal of a plausible antifungal mechanism of this derivative against dermatophytic strains.

The action mechanism of a quinoline derivative was evaluated through the sorbitol protection assay, exogenous ergosterol binding, and the determination of the dose-response curves by time-kill assay. Scanning electron microscopy evaluated the effect of the derivative in the fungal cells. The efficacy of a quinoline-derivative nail lacquer on an onychomycosis model using porcine hooves was evaluated as well.

The quinoline derivative showed a time-dependent fungicidal effect, demonstrating reduction and damage in the morphology of dermatophytic hyphae. In addition, the onychomycosis model was effective in the establishment of infection by .

Treatment with the quinoline-derivative lacquer showed a significant inhibitory effect on strain in this infection model. Finally, the compound presents high potential for application in a formulation such as nail lacquer as a possible treatment for dermatophytic onychomycosis.

Funding
This study was supported by the:
  • FAPERGS (Award 16/2551-0000517-6)
    • Principle Award Recipient: AlexandreMeneghello Fuentefria
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2021-01-27
2021-10-24
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