1887

Journal of Medical Microbiology logo

Volume 70, Issue 3

Phenotypic and genetic characteristics of isolates from site-specific dental plaque in China Free

Abstract

is an important cariogenic microbe.

The potential characteristics of isolates from site-specific dental plaque are still not clear.

This study aimed to investigate the phenotypic and genetic characteristics of isolates from site-specific dental plaque in China.

We used isolated from children with early-childhood caries (ECC) and caries-free children to compare the phenotypic and genetic characteristics of from site-specific dental plaque samples. The ECC subjects presented two sites: a cavitated lesion and a sound surface. The caries-free subjects presented one sound surface. Growth pattern, biofilm, decrease in pH, extracellular polysaccharide, expression levels of virulence-related genes, multilocus sequence typing (MLST) and phylogenetic trees were evaluated among these three sites.

The phenotypes detected between the cavitated and sound surfaces of ECC children were similar. However, the capacity for biofilm formation, pH drop and expression levels of genes ( and ) of in the caries-free group were lower compared with those of the ECC group. We identified 44 new alleles and 77 new sequence types. More than 90 % of the children with ECC shared an identical sequence type. The distribution of sequence types among different subjects showed diversity, and child-to-child transmission was detected.

This is the first report of MLST on site-specific dental plaques in a single subject, and indicates that isolated from site-specific dental plaque of a single subject showed similar phenotypes as a result of the isolates were closely related.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): multilocus sequence typing , phenotypes , site-specific dental plaque and Streptococcus mutans
Funding
This study was supported by the:
  • the Foundation for Innovative Research Groups of Anhui province (Award 2016–40)
    • Principle Award Recipient: Jun-changGuan
  • the Department of Science and Technology of Anhui Province (Award 1804h08020290)
    • Principle Award Recipient: KaiZhang
  • the First Affiliated Hospital of Bengbu Medical College Science Fund for Outstanding Young Scholars (Award 2019byyfyyq07)
    • Principle Award Recipient: ShanshanLiu
  • the Natural Science Fund of Education Department of Anhui province (Award KJ2018A0223)
    • Principle Award Recipient: ShanshanLiu
© 2021 The Authors
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.001313
2021-01-18
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/70/3/jmm001313.html?itemId=/content/journal/jmm/10.1099/jmm.0.001313&mimeType=html&fmt=ahah

References

  1. Anil S, Anand PS. Early childhood caries: prevalence, risk factors, and prevention. Front Pediatr 2017; 5:157 [View Article]
     [Google Scholar]
  2. Piva F, Pereira JT, Luz PB, Hugo FN, de Araújo FB. Caries progression as a risk factor for increase in the negative impact on OHRQOL-a longitudinal study. Clin Oral Investig 2018; 22:819–828 [View Article]
     [Google Scholar]
  3. Righolt AJ, Jevdjevic M, Marcenes W, Listl S. Global-, Regional-, and country-level economic impacts of dental diseases in 2015. J Dent Res 2018; 97:501–507 [View Article][PubMed]
     [Google Scholar]
  4. Du MQ, Li Z, Jiang H, Wang X, Feng XP et al. Dental caries status and its associated factors among 3- to 5-year-old children in China: a national survey. Chin J Dent Res 2018; 21:167–179 [View Article][PubMed]
     [Google Scholar]
  5. Matsui R, Cvitkovitch D. Acid tolerance mechanisms utilized by Streptococcus mutans . Future Microbiol 2010; 5:403–417 [View Article][PubMed]
     [Google Scholar]
  6. Thurnheer T, van der Ploeg JR, Giertsen E, Guggenheim B. Effects of Streptococcus mutans gtfC deficiency on mixed oral biofilms in vitro. Caries research 2006; 40:163–171
     [Google Scholar]
  7. Zhou Y, Millhouse E, Shaw T, Lappin DF, Rajendran R et al. Evaluating Streptococcus mutans strain dependent characteristics in a polymicrobial biofilm community. Front Microbiol 2018; 9:1498 [View Article]
     [Google Scholar]
  8. Crowley PJ, Svensater G, Snoep JL, Bleiweis AS, Brady LJ. An ffh mutant of Streptococcus mutans is viable and able to physiologically adapt to low pH in continuous culture. FEMS Microbiol Lett 2004; 234:315–324 [View Article]
     [Google Scholar]
  9. Lemos José A. C., Brown TA, Lemos JA, Lemos JA, Burne RA. Effects of RelA on key virulence properties of planktonic and biofilm populations of Streptococcus mutans . Infect Immun 2004; 72:1431–1440 [View Article]
     [Google Scholar]
  10. Zhou Y, Yu L, Tao Y, Zhi Q, Lin H. Genetic polymorphism of scrA gene of Streptococcus mutans isolates is not associated with biofilm formation in severe early childhood caries. BMC Oral Health 2017; 17:114 [View Article]
     [Google Scholar]
  11. Zhao W, Li W, Lin J, Chen Z, Yu D. Effect of sucrose concentration on sucrose-dependent adhesion and glucosyltransferase expression of S. mutans in children with severe early-childhood caries (S-ECC). Nutrients 2014; 6:3572–3586 [View Article]
     [Google Scholar]
  12. Nakano K, Ooshima T. Serotype classification of Streptococcus mutans and its detection outside the oral cavity. Future Microbiol 2009; 4:891–902 [View Article]
     [Google Scholar]
  13. Momeni SS, Whiddon J, Cheon K, Moser SA, Childers NK. Assessment of clonality and serotypes of Streptococcus mutans among children by multilocus sequence typing. Eur J Oral Sci 2015; 123:416–424 [View Article]
     [Google Scholar]
  14. Nobile CG, Fortunato L, Bianco A, Pileggi C, Pavia M. Pattern and severity of early childhood caries in southern Italy: a preschool-based cross-sectional study. BMC Public Health 2014; 14:206 [View Article]
     [Google Scholar]
  15. LX Y, Tao Y, Qiu RM, Zhou Y, Zhi QH. Genetic polymorphisms of the sortase a gene and social-behavioural factors associated with caries in children: a case-control study. BMC Oral Health 2015; 15:54
     [Google Scholar]
  16. Richards VP, Alvarez AJ, Luce AR, Bedenbaugh M, Mitchell ML et al. Microbiomes of site-specific dental plaques from children with different caries status. Infect Immun 2017; 85: [View Article]
     [Google Scholar]
  17. Wan AK, Seow WK, Walsh LJ, Bird PS. Comparison of five selective media for the growth and enumeration of Streptococcus mutans . Aust Dent J 2002; 47:21–26 [View Article]
     [Google Scholar]
  18. Shibata Y, Ozaki K, Seki M, Kawato T, Tanaka H et al. Analysis of loci required for determination of serotype antigenicity in Streptococcus mutans and its clinical utilization. J Clin Microbiol 2003; 41:4107–4112 [View Article]
     [Google Scholar]
  19. Kulshrestha S, Khan S, Hasan S, Khan ME, Misba L et al. Calcium fluoride nanoparticles induced suppression of Streptococcus mutans biofilm: an in vitro and in vivo approach. Appl Microbiol Biotechnol 2016; 100:1901–1914 [View Article]
     [Google Scholar]
  20. Liu S, Tao Y, Yu L, Zhuang P, Zhi Q et al. Analysis of small RNAs in Streptococcus mutans under acid Stress-A new insight for caries research. Int J Mol Sci 2016; 17:1529 [View Article]
     [Google Scholar]
  21. Liu SS, Zhu WH, Zhi QH, Liu J, Wang Y et al. Analysis of sucrose-induced small RNAs in Streptococcus mutans in the presence of different sucrose concentrations. Appl Microbiol Biotechnol 2017; 101:5739–5748 [View Article]
     [Google Scholar]
  22. Liu S, Li X, Guo Z, Liu H, Sun Y et al. A core genome multilocus sequence typing scheme for Streptococcus mutans . mSphere 2020; 5: [View Article]
     [Google Scholar]
  23. Do T, Gilbert SC, Clark D, Ali F, Fatturi Parolo CC et al. Generation of diversity in Streptococcus mutans genes demonstrated by MLST. PLoS One 2010; 5:e9073 [View Article]
     [Google Scholar]
  24. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
     [Google Scholar]
  25. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. mega X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549 [View Article]
     [Google Scholar]
  26. Marchesan JT, Morelli T, Moss K, Preisser JS, Zandona AF et al. Interdental cleaning is associated with decreased oral disease prevalence. J Dent Res 2018; 97:773–778 [View Article]
     [Google Scholar]
  27. Vega-Lopez S, Lindberg NM, Eckert GJ, Nicholson EL, Maupome G. Association of added sugar intake and caries-related experiences among individuals of Mexican origin. Community Dent Oral Epidemiol 2018; 46:376–384 [View Article]
     [Google Scholar]
  28. Henne K, Gunesch AP, Walther C, Meyer-Lueckel H, Conrads G et al. Analysis of bacterial activity in sound and cariogenic biofilm: a pilot in vivo study. Caries Res 2016; 50:480–488 [View Article]
     [Google Scholar]
  29. Chen L, Ren Z, Zhou X, Zeng J, Zou J et al. Inhibition of Streptococcus mutans biofilm formation, extracellular polysaccharide production, and virulence by an oxazole derivative. Appl Microbiol Biotechnol 2016; 100:857–867 [View Article]
     [Google Scholar]
  30. Heim KP, Sullan RM, Crowley PJ, El-Kirat-Chatel S, Beaussart A et al. Identification of a supramolecular functional architecture of Streptococcus mutans adhesin P1 on the bacterial cell surface. J Biol Chem 2015; 290:9002–9019 [View Article][PubMed]
     [Google Scholar]
  31. Momeni SS, Whiddon J, Cheon K, Moser SA, Childers NK. Assessment of two multilocus sequence typing (MLST) schemes available for Streptococcus mutans . Arch Oral Biol 2015; 60:1769–1776 [View Article]
     [Google Scholar]
  32. da Silva Bastos V de A, Freitas-Fernandes LB, Fidalgo TK da S, Martins C, Mattos CT et al. Mother-to-child transmission of Streptococcus mutans: a systematic review and meta-analysis. J Dent 2015; 43:181–191 [View Article][PubMed]
     [Google Scholar]
  33. Alves AC, Nogueira RD, Stipp RN, Pampolini F, Moraes AB et al. Prospective study of potential sources of Streptococcus mutans transmission in nursery school children. J Med Microbiol 2009; 58:476–481 [View Article]
     [Google Scholar]
  34. Momeni SS, Whiddon J, Moser SA, Childers NK. Transmission patterns of Streptococcus mutans demonstrated by a combined rep-PCR and MLST approach. Clin Oral Investig 2018; 22:2847–2858 [View Article]
     [Google Scholar]
  35. Lapirattanakul J, Nakano K, Nomura R, Hamada S, Nakagawa I et al. Demonstration of mother-to-child transmission of Streptococcus mutans using multilocus sequence typing. Caries Res 2008; 42:466–474 [View Article]
     [Google Scholar]
  36. Hajishengallis E, Parsaei Y, Klein MI, Koo H. Advances in the microbial etiology and pathogenesis of early childhood caries. Mol Oral Microbiol 2017; 32:24–34 [View Article]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.001313
Loading
Phenotypic and genetic characteristics of Streptococcus mutans isolates from site-specific dental plaque in China
J. Med. Microbiol. 70, 001313 (2021); https://doi.org/10.1099/jmm.0.001313
/content/journal/jmm/10.1099/jmm.0.001313
/content/journal/jmm/10.1099/jmm.0.001313
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error