RT Journal Article SR Electronic(1) A1 de Araújo Longo, Luís Guilherme A1 Fontana, Herrison A1 Santos de Sousa, Viviane A1 Chilinque Zambão da Silva, Natalia A1 Souto Martins, Ianick A1 Meurer Moreira, BeatrizYR 2021 T1 Emergence of mgrB locus deletion mediating polymyxin resistance in pandemic KPC-producing Klebsiella pneumoniae ST15 lineage JF Journal of Medical Microbiology, VO 70 IS 3 OP SP 001309 DO https://doi.org/10.1099/jmm.0.001309 PB Microbiology Society, SN 1473-5644, AB Klebsiella pneumoniae causes a diversity of infections in both healthcare and community settings. This pathogen is showing an increased ability to accumulate antimicrobial resistance and virulence genes, making it a public health concern. Here we describe the whole-genome sequence characteristics of an ST15 colistin-resistant K. pneumoniae isolate obtained from a blood culture of a 79-year-old female patient admitted to a university hospital in Brazil. Kp14U04 was resistant to most clinically useful antimicrobial agents, remaining susceptible only to aminoglycosides and fosfomycin. The colistin resistance in this isolate was due to a ~1.3 kb deletion containing four genes, namely mgrB, yebO, yobH and the transcriptional regulator kdgR. The study isolate presented a variety of antimicrobial resistance genes, including the carbapenemase-encoding gene bla KPC-2, the extended-spectrum beta-lactamase (ESBL)-encoding gene bla SHV-28 and the beta-lactamase-encoding gene bla OXA-1. Additionally, Kp14U04 harboured a multiple stress resistance protein, efflux systems and regulators, heavy metal resistance and virulence genes, plasmids, prophage-related sequences and genomic islands. These features revealed the high potential of this isolate to resist antimicrobial therapy, survive in adverse environments, cause infections and overcome host defence mechanisms., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001309